Tissue-resident memory T cell
A committed tissue-resident memory T cell precursor within the circulating CD8+ effector T cell pool
... OT-I T cells) by FACS sorting on a FACSAria Fusion (BD Biosciences), cells derived from the three mice were mixed 1:1:1 and taken up in PBS supplemented with ...Single Cell 39 ...
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Cross-Presentation of Skin-Targeted Recombinant Adeno-associated Virus 2/1 Transgene Induces Potent Resident Memory CD8+ T Cell Responses
... target tissue. Studies address- ing the ability of rAAV to induce protective T cell responses, however, are ...a tissue-resident memory T cell response has never ...
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Targeting OX40 Promotes Lung-Resident Memory CD8 T Cell Populations That Protect against Respiratory Poxvirus Infection
... central memory that resides primarily in the lymphoid organs or whether it can equally or preferentially promote peripheral tissue memory (so-called effector memory) has not been ...
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Mucosal BCG vaccination induces protective lung-resident memory T cell populations against tuberculosis
... lung-infiltrating T cells were located in the lung parenchyma or the lung airways, we first removed the bronchoalveolar lavage fluid (BALF) and performed flow cytom- etry of the lung parenchyma ...⫹ T cells ...
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Influenza specific lung resident memory T cells are proliferative and polyfunctional and maintain diverse TCR profiles
... the tissue is a major factor shaping the size and repertoire of the Trm cell pool, it is plausible that the HLA type of individuals could affect their capacity to develop Trm ...skin-resident ...
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Resident memory T cells, critical components in tumor immunology
... on T cells residing in tissue microenvironments, where TGF-β is abundant, such as mucosal CD8 + T lymphocytes and, mainly, IEL [53], but it is also expressed on CD4 + and CD8 + regula- tory T ...
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ABC transporters and NR4A1 identify a quiescent subset of tissue resident memory T cells
... of tissue-resident long-lived stem cells is characterized by the ability to efflux Hoechst dyes and is referred to as side population (SP) ...SP T cells (Tsp cells) that exhibit a quiescent (G 0 ) ...
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Vaccine-generated lung tissue–resident memory T cells provide heterosubtypic protection to influenza infection
... Tissue-resident memory T cells (TRM) are a recently defined, noncirculating subset with the potential for rapid in situ protective responses, although their generation and role in vaccine- ...
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Functional heterogeneity of human tissue-resident memory T cells based on dye efflux capacities
... tissues. Tissue samples were maintained in cold saline and brought to the laboratory within 2–4 hours of organ ...spleen tissue using the Bullet Blender Tissue Homogenizer (Next ...Advance). ...
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Interlesional diversity of T cell receptors in melanoma with immune checkpoints enriched in tissue-resident memory T cells
... cells. Tissue and blood samples were collected from mel- anoma patients (clinical characteristics in Table 1) by the Tissue Resource Core of the Yale SPORE in Skin Cancer, with informed written consent ...
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Resident memory CD8+ T cells within cancer islands mediate survival in breast cancer patients
... noncancerous tissue specimens were collected by surgical resection and collected in tubes containing cold RPMI medium (Life Technologies, Thermo Fisher Scientific) and transported on ice to the laboratory for ...
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Mucosal BCG Vaccination Induces Protective Lung Resident Memory T Cell Populations against Tuberculosis
... lung-infiltrating T cells were located in the lung parenchyma or the lung airways, we first removed the bronchoalveolar lavage fluid (BALF) and performed flow cytom- etry of the lung parenchyma ...⫹ T cells ...
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A systematic review: the role of resident memory T cells in infectious diseases and their relevance for vaccine development
... Herpes simplex virus causes infections that present with a varying range of symptoms. The primary clinical manifesta- tions of HSV infection are intraepithelial vesicles. There are two antigenically distinct HSV ...
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Tissue-resident stem cell activity: a view from the adult Drosophila gastrointestinal tract
... intestinal homeostasis. Thus, we next discuss current knowledge of the regionalization of the adult Drosophila intestine. Recently, the adult Drosophila gastrointestinal tract was shown to function as a workhorse for ...
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CD4+ and CD8+ T cell–dependent antiviral immunity requires STIM1 and STIM2
... as LCMV stocks were measured by viral plaque assays using Vero 76 cells as previously described (82). Briefly, confluent monolayers of Vero 76 cells were incubated with dilutions of samples for 1 hour. The sample ...
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Vaccination with live attenuated simian immunodeficiency virus causes dynamic changes in intestinal CD4+CCR5+ T cells
... CD4+CCR5+ T cells ...intestinal T cells is reported to be >60% ...intestinal T cells of SIV natural hosts is reported to be considerably lower those we have ...CD4+ T cells may contribute ...
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Pinilla, Severin (2012): Einfluss mesenchymaler Stammzellen aus dem Fettgewebe auf das Invasionsverhalten muriner und humaner Brustkrebszellen. Dissertation, LMU München: Medizinische Fakultät
... It would be interesting now to see whether MDA MB 231 cells also secrete factors that attract and mobilize adipose tissue derived stem cells. This seems to be very likely according to the findings of the group of ...
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Original Article Migration path of stem cells involved in the repair of damaged pancreatic tissue caused by pancreatitis
... acinar tissue at this time ...islet tissue. Ishiwata et al. [17] also uti- lized nestin as a stem cell-specific marker protein in pancreatic stem cell research and found that nestin-positive ...
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KLRG1 restricts memory T cell antitumor immunity
... For cytokine detection, cells were stimulated with phorbol myristate acetate (50 ng/mL; Sigma-Aldrich), ionomycin (1 µM; Sigma-Aldrich) for 4 hours with protein transport inhibitor (GolgiPlug 1 µl/ml, GolgiStop 2/3 ...
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Compromised Influenza Virus-Specific CD8+-T-Cell Memory in CD4+-T-Cell-Deficient Mice
... was not reflected in any obvious (Fig. 2; Table 1) defect in the level of CTL effector function (in the BAL specimens) or in the capacity to produce IFN-␥ following short-term, in vitro stim- ulation with peptide (in the ...
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