Tobacco etch virus
Thermodynamic Analysis of Binding and Enzymatic Properties of Pokeweed Antiviral Protein (PAP) toward Tobacco Etch Virus (TEV) RNA
... Pokeweed antiviral protein (PAP) from P. americana is a ribosome inactivating protein (RIP) and is an N-glycosidase that removes specific purine residues from the sarcin/ricin (S/R) loop of the large rRNA, arresting ...
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Identification and Characterization of the Functional Elements within the Tobacco Etch Virus 5′ Leader Required for Cap-Independent Translation
... encephalomyocarditis virus, and those that use plants as their host, e.g., tobacco etch virus ...by virus-encoded proteases into capsid and noncapsid proteins that are required to carry ...
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Cap-Independent Translation Conferred by the 5′ Leader of Tobacco Etch Virus Is Eukaryotic Initiation Factor 4G Dependent
... The 5 ⴕ leader of tobacco etch virus (TEV) genomic RNA directs efficient translation from the naturally uncapped viral mRNA. Two distinct regions within the TEV 143-nucleotide leader confer ...
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Tobacco etch virus Protein P1 Traffics to the Nucleolus and Associates with the Host 60S Ribosomal Subunits during Infection
... with Tobacco etch virus (TEV; genus Potyvirus) clones in which P1 was tagged with a fluorescent protein to track its expression and subcellular lo- calization or with an affinity tag to identify host ...
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Fitness Declines in Tobacco Etch Virus upon Serial Bottleneck Transfers
... It has been well established that populations of RNA viruses transmitted throughout serial bottlenecks suffer from significant fitness declines as a consequence of the accumulation of deleterious mutations by the onset ...
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Distribution of Fitness and Virulence Effects Caused by Single-Nucleotide Substitutions in Tobacco Etch Virus
... Dealing with the existence of lethal mutations. Lethal mu- tations and failed inoculation experiments produce the same apparent result: an absence of viral replication in the plant. We failed to infect plants after three ...
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Secondary Structures in the Capsid Protein Coding Sequence and 3′ Nontranslated Region Involved in Amplification of the Tobacco Etch Virus Genome
... The cis-active sequence within the CP-coding region (region A) was functionally resolved into a series of three sequences or structures. First, a perfect 9-bp stem formed exclusively by CP-coding sequence within region A ...
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Changes in the gene expression profile of Arabidopsis thaliana after infection with Tobacco etch virus
... the virus in its own ...that tobacco plants expressing mutant TMV coat proteins triggered the over-expression of HSP as a consequence of the presence of large amounts of denatur- ized proteins in the ...for ...
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Functions of the tobacco etch virus RNA polymerase (NIb): subcellular transport and protein-protein interaction with VPg/proteinase (NIa).
... The NIb protein of tobacco etch potyvirus (TEV) possesses several functions, including RNA-dependent RNA polymerase and nuclear translocation activities. Using a reporter protein fusion strategy, NIb was ...
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Roles of the sequence encoding tobacco etch virus capsid protein in genome amplification: requirements for the translation process and a cis-active element.
... cis-active RNA sequence between CP codons 211 and 246. The mutational analysis presented here revealed an essential function of the CP coding sequence near the 3 9 end of the open reading frame (Fig. 7). The D N210 ...
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Mutational analysis of tobacco etch virus polyprotein processing: cis and trans proteolytic activities of polyproteins containing the 49-kilodalton proteinase.
... The locations of the 49-kDa' proteinase-mediated cleavage sites flanking the 71-kDa cytoplasmic pinwheel inclusion protein, 6-kDa protein, 49-kDa proteinase, and 58-kDa putative polymera[r] ...
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Self assembly of hexahistidine tagged tobacco etch virus capsid protein into microfilaments that induce IgG2 specific response against a soluble porcine reproductive and respiratory syndrome virus chimeric protein
... cess of a PRRSV vaccine relies on its ability to induce both neutralizing epitopes and cellular response [23]. To our knowledge there is no report in the use of TEV CP-based particles, some of them longer than 1 μm, for ...
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Suppression of long-distance movement of tobacco etch virus in a nonsusceptible host.
... element staining may have been due to the actual presence of virus-encoded GUS in the elements or to leakage of the X-gluc product from companion cells. Because of the possibility that the X-gluc product could ...
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Molecular Characterization of the Potyviral First Protein (P1 Protein)
... mosaic virus (TuMV) P1 as ...in virus-infected cells. Besides, the infection ability of Tobacco etch virus (TEV) mutations indicated that P1 may be involved in other non-proteolytic ...
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A translation strategy and gene map for pea seedborne mosaic virus
... Translation of potyvirus RNA in a rabbit reticulocyte lysate: identification of nuclear inclusion proteins of tobacco etch virus RNA translation and cylindrical inclusion protein as a pr[r] ...
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Dissecting the Herpesvirus Architecture by Targeted Proteolysis
... 3. Tobacco etch virus (TEV) protease recognition sites and the GFP coding sequence were inserted into the pBecker3 derivative pGS4284, which encodes a pUL25/mCherry (red fluorescent capsid) fusion, ...
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Crystal Structure of a Novel Dimeric Form of NS5A Domain I Protein from Hepatitis C Virus
... and tobacco etch virus protease cleavage site upstream of the hepatitis C virus NS5A sequence has resulted in a more straightforward purification method and improved yields of purified NS5A ...
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A Flexible Genome-Scale SARS-CoV-2 Clone Resource
... Although our collection facilitates tagging of SARS-CoV-2 proteins for various functional studies, certain applications require removal of tags at some stage, for example, after protein purification. Fusion proteins can ...
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Relocation of the NIb Gene in the Tobacco Etch Potyvirus Genome
... of Tobacco etch virus (TEV) to all possible intercistronic positions in the genome and assayed the infectivity of the resulting recombinant viruses in tobacco (Nico- tiana tabacum ...of ...
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Host-Specific Involvement of the HC Protein in the Long-Distance Movement of Potyviruses
... Viruses. Virus progeny derived from pGPPV (strain D) (53) was used for most of the ...tabacum. Tobacco etch virus (TEV) and the Fny strain of cucumber mosaic virus (CMV) were provided ...
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