Topoisomerase-II-a
Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a / mutants
... top2a mutants exhibit defects in cell cycle progression Topoisomerase II alpha genes are known to be critical for cell cycle progression. Therefore, we quantified, by flow cytometry, the distribution of blm ...
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Polyomavirus minichromosomes: associated DNA topoisomerase II and DNA ligase activities.
... When DNA topoisomerase II activity arbitrary units based on the appearance of completely unknotted bacteriophage P4 DNA molecules was computed relative to the total number of polyomaviru[r] ...
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Association between the p170 form of human topoisomerase II and progeny viral DNA in cells infected with herpes simplex virus type 1.
... In the present study, we show that topoisomerase II activity persists in HSV-1-infected cells and that both forms are present throughout the infection; however, only p170 topoisomerase I[r] ...
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The significance of Epstein Barr Virus (EBV) & DNA Topoisomerase II alpha (DNA-Topo II alpha) immunoreactivity in normal oral mucosa, Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC)
... DNA-Topo II∝ was expressed in all examined ...DNA-Topo II∝ expression was significantly higher in tumors of low differentiation versus tumors of moderate and high differentiation (P < ...DNA-Topo ...
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The role of NF Y in the transcriptional regulation of human topoisomerase II¯ : a thesis presented to Massey University in partial fulfilment of the requirement for the degree of Master of Science in Biochemistry
... Resistance to various topoisomerase targeting drugs has been observed with factors as candidates for the production of resistant cancer cells with respect to topoisomerase II targeted dr[r] ...
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Topoisomerase II and other DNA-delay and DNA-arrest mutations impair bacteriophage T4 DNA packaging in vivo and in vitro.
... Temperature shift and in vitro packaging experiments with the am39 mutant indicated that packaging of normal DNA occurs in the absence of topoisomerase II function, yet in vivo and in vi[r] ...
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A role for topoisomerase II alpha in chromosome damage in human cell lines
... topo II action involves DSB induction, the DSBs are protein-associated and the DSB is usually religated soon after, so no overall damage will have ...topo II might mis-join the ends of the ...topo II ...
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The role of NF Y in the transcriptional regulation of human topoisomerase II¯ : a thesis presented to Massey University in partial fulfilment of the requirement for the degree of Master of Science in Biochemistry
... Resistance to various topoisomerase targeting drugs has been observed with factors as candidates for the production of resistant cancer cells with respect to topoisomerase II targeted dr[r] ...
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In silico identification and evaluation of hits for the inhibition of topoisomerase-ii enzyme via structure based pharmacophore mapping and virtual screening
... The ligand based pharmacophore model of the 10 training sets of previously reported topoisomerase II inhibitors was generated by using MOE 2009.10 pharmacophore constructing tool. Binding interactions ...
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Assessment of drug induced genotoxicity in mammalian cells and the contribution of topoisomerase II inhibition
... or topoisomerase II ...research, topoisomerase II inhibition is a particularly interesting genotoxic mechanism, as the enzyme is used as a target for both oncology and anti-infective ...some ...
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PODOPHYLLOTOXIN AND THEIR GLYCOSIDIC DERIVATIVES
... between topoisomerase II and DNA that is referred to as the cleavable ...that topoisomerase II was the most likely cellular target in the double stranded breaking activity of ...
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cGAS/STING axis mediates a topoisomerase II inhibitor–induced tumor immunogenicity
... (Figure 2A and Supplemental Figure 2, A and B). Moreover, it also induced tumor cell death, which was detected by flow cytometry, lactate dehydrogenase (LDH) release (Figure 2B and Supplemen- tal Figure 2, C and D), and ...
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Regulation of DNA Damage Signaling and Cell Death Responses by Epstein-Barr Virus Latent Membrane Protein 1 (LMP1) and LMP2A in Nasopharyngeal Carcinoma Cells
... ble-strand breaks (DSBs), but at low doses it also can induce single-strand breaks (SSBs) by incomplete inhibition of topo- isomerase II homodimers (48). In order to investigate DNA dam- age mechanisms that may ...
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The ATP dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning
... Deletion of ULS1 does not result in a dramatic growth de- fect or in sensitivity to a variety of DNA damaging drugs (Supplementary Figure S1A). This apparent absence of phenotype initially hindered our attempts to ...
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Genetic analysis of the gyrase A-like domain of DNA topoisomerase II of Saccharomyces cerevisiae.
... used tetrad analysis to group the extragenic suppres- sor mutations. Tetrad analysis showed that seven of the 16 tos mutations fell into one linkage group, which we refer to[r] ...
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SIZ1/SIZ2 Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II
... The results showing that the simultaneous loss of Siz1p and Siz2p activity is epistatic to the top2 SNM al- leles and yields an unmodified Top2p suggest that the role of SUMO E3 in chromosome segregation could be limited ...
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Topoisomerase II Inhibitors Induce DNA Damage Dependent Interferon Responses Circumventing Ebola Virus Immune Evasion
... Doxorubicin and daunorubicin induce IFN by an ATM-dependent mechanism. Doxorubicin has pleiotropic effects on cells. Among its activities, it is a topoisomerase II poison that intercalates into DNA, ...
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Hypersensitive Inhibition of the Proliferation of Cells with Mutated DNA Repair Related Genes by the Catalytic Topoisomerase II Inhibitor 20 O IngenolEZ
... exhibit topoisomerase inhibitory ...both topoisomerase II (topo II) activity and cell proliferative ...activity. Topoisomerase II inhibitors can be divided into the poison and ...
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Prognostic Values of Filamin-A Status for Topoisomerase II Poison Chemotherapy
... Topo II poisons than filamin-A proficient ...Topo II poisons. Filamin-A proficient cells incurs more Topo II poison-induced DNA damages compared to filamin-A deficient cells, due to elevated ...
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Selective induction of apoptosis in Hep 3B cells by topoisomerase I inhibitors: evidence for a protease dependent pathway that does not activate cysteine protease P32
... whether topoisomerase-directed drugs would in- duce apoptosis in a human HCC cell line (Hep 3B) and, if so, to investigate the ...The topoisomerase I poi- son camptothecin (CPT) induced apoptosis of Hep 3B ...
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