Toxins A and B
Ultrasensitive Detection of Clostridioides difficile Toxins A and B by Use of Automated Single Molecule Counting Technology
... Clinical evaluation. The study was approved by the institutional review board at Stanford University (Palo Alto, CA, USA) (protocol IRB-43749). Frozen stool samples from 311 patients with suspected CDI, collected at the ...
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Harnessing the Glucosyltransferase Activities of Clostridium difficile for Functional Studies of Toxins A and B
... the toxins were analyzed using the ProtParam program (20) to assess their physicochemical ...Toxin B has more charged residues (597 of a total of 2,366 residues), and 66% and 34% are negatively and posi- ...
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Toxins A and B from Clostridium difficile differ with respect to enzymatic potencies, cellular substrate specificities, and surface binding to cultured cells
... these toxins, at least in Don and T84 cells (see ...the toxins directly into the cytosol, the binding and internalization processes were ...7, B and ...
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Enzyme linked immunosorbent assays for Staphylococcus aureus exfoliative toxins A and B and some applications
... Our report describes two simple, specific, and sensitive 3 ng/ml ELISA procedures which require affinity-purified toxin antibodies and which can be used to determine the ET concentration[r] ...
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Enzyme immunoassay for detection of antibody to toxins A and B of Clostridium difficile
... Relationship between ELISA value corrected absorbance and titer measured by the cytotoxicity neutralization assay for anti-B antibody in 27 sera from immunized hamsters and 28 sera from [r] ...
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Profiling Humoral Immune Responses to Clostridium difficile-Specific Antigens by Protein Microarray Analysis
... In the CDI group, antibody responses to whole Figure 4A and B and recombinant toxins A and B Figure 5 did not differ compared with the healthy control group, but infected patients did ex[r] ...
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The Eukaryotic Host Factor 14 3 3 Inactivates Adenylate Cyclase Toxins of Bordetella bronchiseptica and B parapertussis, but Not B pertussis
... the expression of IL-6 and TNF- ␣ in L2 and NR8383 (rat alveolar macrophage) cells using real-time quantitative reverse transcription-PCR but observed no significant differences between B. pertussis CyaA and ...
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Toxins and drug discovery
... exactly toxins and from sources that are not exactly ...translating toxins to products in recent years have been the developments from the microbial botulinum ...botulinum toxins A and B have ...
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Association of Clostridium difficile with Antibiotic Associated Diarrhea among Hospitalized Children in Diyala Iraq
... toxin B, and both A and B of ...toxin B was not detected in both patients and ...difficile toxins A and B directly in fecal specimens or in toxigenic cultures were widely acceptable ...
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Stx2 Subtyping of Shiga Toxin Producing Escherichia coli Isolated from Cattle in France: Detection of a New Stx2 Subtype and Correlation with Additional Virulence Factors
... Mouse and human intestinal mucus is able to activate Stx2vh-a and Stx2vh-b toxins but not Stx2-EDL933 or Stx2c (12). E. coli B2F1, producing both Stx2vh-a and Stx2vh-b, is highly virulent in an ...
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Toxins as biological weapons for terror—characteristics, challenges and medical countermeasures: a mini-review
... Biological warfare is defined as the intentional use of liv- ing organisms such as bacteria, viruses and fungi with the intent to cause disease, death, or environmental damage [1, 2]. Pathogens were classified by the ...
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Meeting abstracts from International Conference on Prevention & Infection Control (ICPIC 2017)
... Methods: Stool samples were screened for CD toxins A/B by immuno- assay (Vidas® C. difficile A&B, bioMérieux) and notified to the Hospital Hygiene Service (HHS). Since the beginning [r] ...
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Clostridium difficile toxin A stimulates intracellular calcium release and chemotactic response in human granulocytes
... toxin B, a ...difficile toxins on activation of human ...toxin B, elicited a significant chemotactic and chemokinetic response by granulocytes that was comparable with that induced by the chemotactic ...
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An Investigation on T-2 Toxin Contamination in Wheat Crops from 11 Cities, Iran
... Fusarium toxins the criteria for toxin selection have been: Of the toxins most commonly found in analytical surveys of cereals in the evaluation of Fusarium toxins the criteria for toxin selection ...
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Antimicrobial Peptides, Polymorphic Toxins, and Self Nonself Recognition Systems in Archaea: an Untapped Armory for Intermicrobial Conflicts
... Most of the respective genomic neighborhoods include a gene for a predicted transcriptional regulator of the cI/cro helix-turn-helix (cHTH) family (arCOG04915), which is a common component of toxin-antitoxin systems (45, ...
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A Study of Clinical Profile of Oleander Seed Poisoning in Adults
... Table 1: Toxins in oleander10,19,20 Nerium oleander Thevetia peruviana Oleandrin Thevetin A Folineriin Thevetin B Adynerin Thevetoxin Digitoxigenin Nerifoliin Peruvoside Ruvoside Flowers[r] ...
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Sensitivity of single-molecule array assays to detect Clostridium difficile toxins in comparison to conventional laboratory testing algorithms.
... amphotericin B (Thermo Fisher Scientific, Waltham, MA) at 1/6 dilution and centrifuged at 3,000 ⫻ g for 10 ...antitoxin B Ab (Meridian Life Science, Memphis, TN) was used to verify that the cytotoxic ...
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Sensitivity of Single Molecule Array Assays for Detection of Clostridium difficile Toxins in Comparison to Conventional Laboratory Testing Algorithms
... toxin B assays according to Quanterix methods, using raw materials similar to the ones described by Song et ...toxin B assays have LOD of ...native toxins, which are used as calibrators, and the ...
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Caspase Activation as a Versatile Assay Platform for Detection of Cytotoxic Bacterial Toxins
... Bacterial toxins are a major class of virulence factors and hence are attractive therapeutic targets for vaccine ...bacterial toxins. We have exploited the ability of these toxins to cause cell death ...
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Coral venom toxins
... 1. Toxins have been characterized from four of the Anthozoa order and examples of these toxins are given in Table 1 to highlight the structural diversity, range of bioactivities and potential ...into ...
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