Trabecular meshwork cells
Original Article miR-192-5p, TGFβ2 and the expression of extracellular matrix moieties in of human trabecular meshwork cells
... in trabecular meshwork (TM) especially juxtacanalicular tissue (JCT), is a major fac- tor that contributes to primary open angle glaucoma ...the trabecular meshwork so the obstruction of the ...
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Gene transfer to human trabecular meshwork cells in vitro and ex vivo using HIV-based lentivirus
... Human Trabecular Meshwork Cells The culture of HTM cells was briefly described as follows: Trabecular specimens were obtained less than 24h ...dissected trabecular tissue were ...
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Glucocorticoids with different chemical structures but similar glucocorticoid receptor potency regulate subsets of common and unique genes in human trabecular meshwork cells
... in trabecular meshwork ...TM cells requires further study, however, gene expression analysis revealed that 1,968 and 1,150 genes were regulated by DEX, FA, and TA in TM 86 and TM 93 cells, ...
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Original Article Effects of transforming growth factor-β2 on myocilin expression and secretion in human primary cultured trabecular meshwork cells
... Trabecular meshwork cells (third passage) were identified by transmission electron microscopy and immunocytochemical ...the cells were fixed with a mixture of ...the cells were fixed in ...
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A Role for Herpesvirus Entry Mediator as the Receptor for Herpes Simplex Virus 1 Entry into Primary Human Trabecular Meshwork Cells
... TM cells (indicated as tests) were used as ...TM cells. (B) Cell surface expression of HVEM in TM cells detected by fluorescence-activated cell sorter (FACS) ...TM cells were incubated at 4°C ...
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Minocycline is cytoprotective in human trabecular meshwork cells and optic nerve head astrocytes by increasing expression of XIAP, survivin, and Bcl-2
... unviable cells appear red because of staining by the membrane-impermeable dye, propidium iodide (Sigma-Aldrich, Steinheim, Germany), while the nuclei of all cells were stained with the membrane- permeable ...
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Original Article MiR-184 prevents chronic oxidative stress induced human trabecular meshwork cells apoptosis and cytotoxicity in vitro by targeting hypoxia-inducible factor 1α
... The neurodegenerative protection roles of HIF- 1α in retinal ganglion cells and in glaucoma had been indicated in previous studies [40-43]. The elevated HIF-1α level had been detected in diabetic retina [42], ...
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Experimental research on the relationship between the stiffness and the expressions of fibronectin proteins and adaptor proteins of rat trabecular meshwork cells
... The total protein was extracted with the RIPA (Apply- gen, Beijing, China) from TM cells subjected to different experimental conditions. The concentration of total proteins was monitored with BCA Protein Assay Kit ...
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Original Article αB crystalline upregulates the expression of matrix metalloproteinases in trabecular meshwork cells through TLR1/2
... TM cells were purchased from ScienCell (6590), cultured in DMEM (Gibco 11965-092, USA) supplemented with 10% fetal bovine serum (Gibco 10099141, Australia), 100 U/ml penicillin and 100 ug/ml streptomycin ...
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The intrinsic stiffness of human trabecular meshwork cells increases with senescence
... While the mechanisms underlying senescence- associated stiffening remain incompletely defined, it is likely cytoskeletal reorganization plays a key role. In numerous other studies, senescent cells or those from ...
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Regulation of Phosphatase and Tensin Homolog Expression and Activity by Transforming Growth-Factor Beta in the Trabecular Meshwork Cells: Implications for Primary Open Angle Glaucoma
... incubator. Cells were used at passage ...Once cells reached 90% confluency, the medium was replaced with DMEM supplemented with ...hours cells were pretreated with specific small molecule inhibitors, ...
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Impact of Pigment Dispersion on Trabecular Meshwork Cells
... morphological changes in TM cells, which gradually gained an elongated and polygonal appearance within 1 week (Figure 1E, F, G, J). H&E staining revealed that most of the phagocytosed pigment particles were ...
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Original Article Effects of fasudil on the cytoskeleton of trabecular meshwork cells and outflow facility in bovine eyes
... TM cells were inoculated in 24-well plates and treated with fasudil at different con- centrations (1 μM, 10 μM, 100 μM and 1 mM), respectively, TM cells incubated with PBS were set as ...TM cells ...
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PubMedCentral-PMC4991020.pdf
... For the first time, using the comprehensive NEIGHBORHOOD data set, we have identified a significant association of rs76481776 in MIR182 gene with POAG, especially with the HTG subset. We have confirmed the expression of ...
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Zika Virus Infects Trabecular Meshwork and Causes Trabeculitis and Glaucomatous Pathology in Mouse Eyes
... ganglion cells (RGCs) and cells in the inner nuclear layer, resulting in focal retinitis and optic nerve head infection, and caused thinning of the outer plexiform layer ...
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Microhook ab interno trabeculotomy, a novel minimally invasive glaucoma surgery
... meshwork over 3 clock hours. Using the same procedure, LOT is performed in the opposite angle using a microhook that is inserted through another corneal port. After the vis- coelastic material is aspirated, the ...
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Intraoperative optical coherence tomography and ab interno trabecular meshwork surgery with the Trabectome
... After a temporal 1.7 mm clear cornea incision, the Trabec- tome single-use handpiece was inserted into the anterior chamber and the ACA was visualized using the modified Swan-Jacob goniolens (Ocular Instruments, ...
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A Pilot Study on Human CYP1B1 Gene Mutations in Three Cases with Primary Congenital Glaucoma in Calabar: Benefits for Disease Management
... the major candidate gene participating in trabecular meshwork development leading to ocular abnormality [6] if there is alteration or mutations of the gene [7,18-22]. We observed g.291G>C missense ...
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Microstructure visualization of conventional outflow pathway and finite element modeling analysis of trabecular meshwork
... to inspect ocular tissues. As one of the frequently-used clinical diagnostic equipment, optical coherence tomography (OCT) has a resolution of 15–25 μm [8], while the light scattering properties of sclera causes imaging ...
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Primary extranodal marginal zone B-cell lymphoma with diffuse uveal involvement and focal infiltration of the trabecular meshwork: a case report and review of literature
... and trabecular meshwork infiltrated with malignant cells (Figure ...malignant cells to the retrobulbar tissue was also noted (Figure ...positive cells), whereas CD10, ...
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