Transfected cells
Aquaporin-4 antibody testing: direct comparison of M1-AQP4-DNA-transfected cells with leaky scanning versus M23-AQP4-DNA-transfected cells as antigenic substrate
... formalin-fixed cells were used. A previous study suggested that use of live cells might fur- ther improve AQP4-IgG detection rates ...because cells have to be newly transfected before each run ...
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Upregulation of cellular glutathione levels in human ABCB5- and murine Abcb5-transfected cells
... fected cells showed resistance to anthracyclines and tax- anes ...ABCB5-transfected cells to vari- ous anticancer agents and cytotoxic compounds, and found that the ABCB5-transfected ...
10
Aquareovirus protein VP6 colocalizes with NS80 protein in infected and transfected cells
... in transfected cells appeared differ- ent from its distribution in viral infected ...in transfected cells, all the cell lysates were subjected to SDS-PAGE, followed by immunoblotting with ...
9
Replication of poliovirus RNA and subgenomic RNA transcripts in transfected cells.
... By preparing mutant RNAs with in-frame deletions in the P1 region of the genome, Kaplan and Racaniello 14 showed that most of the sequence that encodes the capsid proteins was not requir[r] ...
9
Splicing of Rhesus Rhadinovirus R15 and ORF74 Bicistronic Transcripts during Lytic Infection and Analysis of Effects on Production of vCD200 and vGPCR
... siently transfected CHO cells were stained for surface-ex- pressed vCD200 with anti-FLAG antibody and subjected to FACS ...vector-transfected cells, cells ex- pressing vCD200 from the ...
5
The level of RECQL1 expression is a prognostic factor for epithelial ovarian cancer
... was transfected into OVCAR-3 ...siRNA transfected-cells significantly re- duced cell growth and increased apoptosis compared to those of control ...cancer cells transfected with RECQL1 ...
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Herpes Simplex Virus 2 Expresses a Novel Form of ICP34.5, a Major Viral Neurovirulence Factor, through Regulated Alternative Splicing
... the cells were pyknotic and the cytoplasm was considerably smaller than at 5 ...Vero cells is consistent in loca- FIG 3 ...in transfected cells. Vero cells were transfected with ...
11
Replication of flock house virus RNAs from primary transcripts made in cells by RNA polymerase II.
... in cells transfected with either of the FHV1 plasmids alone ...in cells cotransfected with both plasmids ...in cells that had been transfected with FHV2 plasmids alone ...in ...
5
Apoptosis of Liver-Derived Cells Induced by Parvovirus B19 Nonstructural Protein
... liver cells, we generated a fusion protein of NS1 with enhanced green fluorescent protein (eGFP) in a system allowing for inducible gene ...HepG2 cells with the eGFP/NS1 vector led to apoptosis of 35% of ...
8
miRNAs associated with chemo-sensitivity in cell lines and in advanced bladder cancer
... cancer cell lines to avoid any single cell line-dependent phenomenon and to enable drawing of more general con- clusions. miRNA down regulation was verified by qRT- PCR following knockdown using two LNA-anti-miRs ...
11
CHL1 suppresses tumor growth and metastasis in nasopharyngeal carcinoma by repressing PI3K/AKT signaling pathway via interaction with Integrin β1 and Merlin
... NPC cells. (A) Ectopic expression of CHL1 was detected in CHL1-transfected cells by qRT-PCR and western ...non-transfected cells were compared by MTT ...NP69 cells by MTT ...of ...
14
Feline Calicivirus: Recovery of Wild-Type and Recombinant Viruses after Transfection of cRNA or cDNA Constructs
... fluorescent cells per ...positive cells had a round shape that was most likely due to CPE (data not ...which transfected cells were superinfected with ...the cells showed MVA-T7- ...
10
Original Article MicroRNA-1284 enhances radio-sensitivity in hepatocellular carcinoma cells by regulating SP1
... resistant cells were ...four cells was monitored by quantitative PCR ...was transfected into cells, and transfected cells viability and radio-sensitivity were measured by Cell ...
8
Complex interaction between dengue virus replication and expression of miRNA 133a
... in transfected cells ...dendritic cells and macrophages, but not in non-hematopoietic cell types ...Huh7 cells was found to decrease the infect- ivity of DENV ...
12
RNA transfected dendritic cells in cancer immunotherapy
... RNA transfected into cells and leads to over a 1,000-fold increase in reporter gene expression in transfected cells ...be transfected in vitro using naked RNA ...DCs transfected ...
6
Construction and Characterization of a Human T-Cell Lymphotropic Virus Type 3 Infectious Molecular Clone
... the cells were washed with phos- phate-buffered saline and fixed for ultrastructural analyses as previously described ...-transfected cells by electron microscopy (Fig. 4A) but not in the ...
6
The Exercise Attitudes, Perceptions, and Perceived Outcomes of Older Minority Women Participating in a Fall Prevention Program
... stably transfected cells despite the clear apoptotic effects in ...Vero cells transfected with P150-GFP were almost confluent and thus normally the majority of cells in the culture ...
86
BAP1 suppresses lung cancer progression and is inhibited by miR-31
... A549 cells (Figure 3B and ...was transfected into A549 cells along with the miR-31 mimic, miR-31 inhibitor or scrambled negative control ...with cells treated with the control mimic, whereas ...
12
High-Efficiency Utilization of the Bovine Integrin αvβ3 as a Receptor for Foot-and-Mouth Disease Virus Is Dependent on the Bovine β3Subunit
... analyzed by RIP and SDS-PAGE as described in Materials and Methods. In all of these assays, equal numbers of TCA-pre- cipitable cpm and equal amounts of protein were immunopre- cipitated within each experiment. This ...
9
The Reflected-Shifted-Truncated-Gamma Distribution for Negatively Skewed Survival Data with Application to Pediatric Nephrotic Syndrome
... cancerous cells invading other organs, then its rapid detection would be very useful in the clinical setting for early screening and ...CT26-FL3 cells, a highly metastatic cell line, the gene encoding the ...
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