Transfection using cationic liposomes
Preparation, characterization, and efficient transfection of cationic liposomes and nanomagnetic cationic liposomes
... nonviral transfection systems for gene ...However, using CLs to deliver therapeutic nucleic acids and drugs to target organs has some problems, includ- ing low transfection efficiency in ...Magnetic ...
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Evaluation of Small Interfering RNA Delivery into Cells by Reverse Transfection in Suspension with Cationic Liposomes
... siRNA transfection method using cationic liposomes, we compared the transfection efficiency of siRNA between Fw-transfection and Rev-transfection methods with various ...
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Type and composition of surfactants mediating gene transfection of polyethylenimine-coated liposomes
... vectors, cationic liposomes 1–3 and polycations 4,5 have been widely ...investigated. Cationic liposomes form a complex with anionic DNA molecules and are thought to deliver DNA through the ...
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Higly fusogenic cationic liposomes transiently permeabilize the plasma membrane of HeLa cells
... Abstract: Cationic liposomes can efficiently carry nucleic acids into mammalian ...diC14-amidine liposomes displayed a much higher susceptibility to lipid mixing with the target ...detected ...
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Cationic vesicles for efficient shRNA transfection in the MCF-7 breast cancer cell line
... many cationic liposomes studied, a few formula- tions have the chance for clinical ...some cationic lipids is an important problem in the application of ...ferent cationic liposomes, 36 ...
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Targeting of small molecule anticancer drugs to the tumour and its vasculature using cationic liposomes: lessons from gene therapy
... for transfection of cul- tured cells and gene transfer in animals, the realisation that they had limitations, prompted a re-evaluation of their ...vivo, transfection results are only slightly better than ...
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Arginine-based cationic liposomes for efficient in vitro plasmid DNA delivery with low cytotoxicity
... the cationic assemblies is influenced by the length 28 and saturation level 29 of the hydrophobic alkyl chain, type of amino acid in the cationic head group, 30 and the characteristics of the ...the ...
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Original Article Anti-EGF antibody cationic polymeric liposomes for delivery of the p53 gene for ovarian carcinoma therapy
... and cationic liposomes, we have developed an efficient targeted gene delivery ...gene transfection efficiency in SKOV3 ...of transfection effi- cacy, decreased cytotoxicity, and efficient tr- ...
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Cationic liposomes induce cytotoxicity in HepG2 via regulation of lipid metabolism based on whole-transcriptome sequencing analysis
... presented a systems biology approach based on next-generation whole transcriptome sequencing coupled with computational methods, including GO en- richment, pathway and co-expression analysis to reveal key roles involved ...
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Complexes containing cationic and anionic pH-sensitive liposomes: comparative study of factors influencing plasmid DNA gene delivery to tumors
... Keywords: cationic liposomes, pH-sensitive liposomes, pDNA, transfection, PEGylated Introduction Gene delivery is a potent therapeutic strategy in tumor ...of cationic liposomes ...
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LIPOSOMES: AN OVERVIEW
... 2. Liposomes in bioengineering Modern genetic engineering and gene recombinant technology is based on the delivery of genetic material, ...others, liposomes were tried as transfection ...into ...
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Transferrin-PEG-PE modified dexamethasone conjugated cationic lipid carrier mediated gene delivery system for tumor-targeted transfection
... circulating liposomes exert strong inhibitory effects on tumor growth in a low dose and low frequency schedule after intravenous ...by using steroid ...
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Synthesis of cationic porphyrins as potential gene transfection carriers
... Porphyrins are found having strong but reversible interaction with nucleic acid and thus can be used as complementary to other non-viral vectors in gene transfection delivery. Amphiphilic porphyrins with ...
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Enhanced localization of anticancer drug in tumor tissue using polyethylenimine conjugated cationic liposomes
... DSPE-PEI cationic liposomes was found to result in significantly increased in vitro intracellular uptake compared with control ...drug-loaded liposomes at the tumor site through electrostatic ...
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Synthesis and bioassay studies of cationic porphyrins for gene transfection delivery
... of cationic porphyrins with nucleic acid and their fluorescent properties for location identification in cellular domain have promoted their uses as potential gene vectors for gene ...basic cationic ...
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Comparative study of the in vitro and in vivo characteristics of cationic and neutral liposomes
... of cationic lipids in liposomes also induces aggregation through electrostatic interactions between the liposomes and the anionic species in the circulation, which results in a reduced EPR ...of ...
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Transfection of KB Cells by Liposomes Containing Adenovirus Type 2 DNA
... Biological properties of poliovirus encapsulated in lipid vesicles: antibody resistance and infectivity in virus- resistant cells. The introduction of poliovirus RNA into cells via lipid[r] ...
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In vitro transfection of bone marrow-derived dendritic cells with TATp-liposomes
... DC transfection are due to the low penetration of DNA into the cytoplasm due to rapid degradation in endosomes or by specific mechanisms protecting cells against foreign ...
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Cationic liposomes (Lipofectin) mediate retroviral infection in the absence of specific receptors.
... Ecotropic murine leukemia virus and packaged retroviral vectors were shown to infect mink cells, and amphotropic packaged retroviral vectors were shown to infect hamster cells in the pre[r] ...
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Mechanisms of Enhanced Antigen Delivery to Murine Dendritic Cells by the Cationic Liposomes
... DOTAP/DC-chol liposomes enable den- dritic cells to internalize antigenic ...the cationic liposomes could be a possible mechanism behind the induc- tion of antigen-specific immune responses by ...
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