Tsetse Fly
Expression and extracellular release of a functional anti-trypanosome Nanobody® in Sodalis glossinidius, a bacterial symbiont of the tsetse fly
... To date, genes involved in insect immunity have attracted most attention as potential antitrypanocidal effectors. In Glossina morsitans morsitans, the host defense genes attacin and defensin are up-regulated in response ...
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Expression of chemosensory proteins in the tsetse fly Glossina morsitans morsitans is related to female host-seeking behaviour
... Genomic DNA was isolated to make standard curves for qPCR. The body tissues of a tsetse fly without the head were homog- enized in a 1.5-mL Eppendorf tube with 250 ml DNA extraction buffer (100 mM Tris-HCl ...
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Delivery of a functional anti-trypanosome Nanobody in different tsetse fly tissues via a bacterial symbiont, Sodalis glossinidius
... Measuring in vivo Sodalis and Wigglesworthia density At various time points post-injection flies were sacrificed for genomic DNA extraction using the QIAGEN DNeasy extraction kit (QIAGEN). We used a quantitative PCR ...
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The relative contributions of developmental plasticity and adult acclimation to physiological variation in the tsetse fly, Glossina pallidipes (Diptera, Glossinidae)
... In this study we therefore examine the effects of altering treatment temperatures during pupal development (developmental plasticity), during the adult stage (adult acclimation) and during both stages (combined ...
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Variations in the Peritrophic Matrix Composition of Heparan Sulphate from the Tsetse Fly, Glossina morsitans morsitans
... Abstract: Tsetse flies are the principal insect vectors of African trypanosomes—sleeping sickness in humans and Nagana in ...the tsetse fly species, Glossina morsitans morsitans, is host to the ...
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Tsetse fly (Glossina pallidipes) midgut responses to Trypanosoma brucei challenge
... infected tsetse fly (Diptera: ...of tsetse and exclusive haematophagy of both ...fective tsetse bites in game parks [5], there is ongoing risk of re-emergence of rhodesiense disease due to the ...
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Temporal genetic differentiation in Glossina pallidipes tsetse fly populations in Kenya
... populations across longer time scales than previously re- ported. We focus on two regions of southwestern Kenya, Nguruman Escarpment (Nguruman) and Ruma National Park (Ruma), that share similarities in ecology and ...
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CONCEALED TRANSEPITHELIAL POTENTIALS AND CURRENT RECTIFICATION IN TSETSE FLY MALPIGHIAN TUBULES
... tsetse fly Malpighian tubules. Some preliminary data have already been reported in a review of ion transport mechanisms in insect tubules (Nicolson, 1993). Using the microperfusion technique developed for ...
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Characterisations of odorant-binding proteins in the tsetse fly Glossina morsitans morsitans
... of tsetse fly are obligate ...of tsetse flies form ‘following swarms’ in which non- feeding males gather around moving objects (normally host animals) in order to intercept females coming to feed ...
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Sticky small target: an effective sampling tool for tsetse fly Glossina fuscipes fuscipes Newstead, 1910
... condition of the flies in subsequent processing such as counting and dissections [13]. As opposed to the sticky substances coated on the screens of the earlier sticky traps, the transparent sticky film used in this study ...
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Prevalence of Bovine Trypanosomosis and Apparent Density of Tsetse Fly in Botor Tolay District, Jimma Zone, Ethiopia
... The mean PCV values recorded were 18.34% in parasitaemic and 24.97% in aparasitaemic animals. And low Pcv animals also found to be found significant compare with other risk factors age and sex which statistically ...
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Tsetse Fly (G f fuscipes) distribution in the Lake Victoria Basin of Uganda
... the tsetse fly. The tsetse vector is distributed across sub-Saharan ...of tsetse flies assists in predicting trypanosomiasis distributions and developing rational strategies for disease and ...
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A new asymmetric division contributes to the continuous production of infective trypanosomes in the tsetse fly
... a tsetse fly is a slow and complex ...of tsetse flies (1 to 9 months) (Aksoy et ...to tsetse salivary glands ...the tsetse flies into efficient and long-lasting vectors for African ...
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Synthesis of tsetse fly attractants from a cashew nut shell extract by isomerising metathesis
... Cashew nut shell liquid (CNSL) is a by-product of cashew nut processing, and is available on a scale of 450 000 tonnes per year. 1 It is an excellent representative of an unused, non- eatable natural resource for which ...
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Electrical transients in the cell volume response to cyclic AMP of the tsetse fly Malpighian tubule
... been observed in the proximal tubule of the mammalian kidney (Laprade, 1994), following both iso-osmotic and hypo-osmotic volume perturbation (luminal addition of glucose and alanine, or hypo-osmotic shock, ...
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Study of distribution and abundance of tsetse fly (glossina sp ) in gashaka gumti national park, Nigeria
... to tsetse fly infestation exists in most national parks/wildlife park and is a major health risk to tourists coming to tropical Africa (Sabbah et ...
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Comparative and functional analysis of tsetse fly Aquaporins
... Homologs of Drip (named GfmAQP1, GffAQP1 and GlAQP1) from three tsetse fly species (G. f. fuscipes, G. f. martini and G. longipennis) were amplified and cloned into pGEM-T Easy cloning vector and sequenced. ...
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The life cycle of Trypanosoma (Nannomonas) congolense in the tsetse fly
... the tsetse fly from the midgut to the mouthparts via the proventriculus and foregut, but the two strains used here evidently differ in their ability to complete the developmental cycle in ...
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Diuresis in the tsetse fly Glossina austeni
... This investigation has shown that during the first hour of diuresis the tsetse fly excretes much of the excess water and salts of the blood meal and, though this must require a rapid flo[r] ...
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Fluorescent proteins reveal what trypanosomes get up to inside the tsetse fly
... the tsetse fly and the small numbers of trypanosomes available for ...the fly [8, 9], and as these are difficult to culture in vitro , experi- ments on genetic exchange involve co-transmission of the ...
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