Tumor Necrosis Factor Gene Expression
Regulation of tumor necrosis factor gene expression by ionizing radiation in human myeloid leukemia cells and peripheral blood monocytes
... Transcriptional run-on analyses have demonstrated that ionizing radiation stimulates the rate of TNF gene transcription. Furthermore, induction of TNF mRNA was increased in the absence of protein synthesis. In ...
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Transcriptional and posttranscriptional regulation of tumor necrosis factor gene expression in human monocytes
... exposure. The protein synthesis inhibitor, cycloheximide (CHX), had no detectable effect on levels of TNF transcripts in resting monocytes, while this agent superinduced the level of TNF mRNA by 50-fold in TPA-activated ...
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Transdominant mutants of I kappa B alpha block Tat-tumor necrosis factor synergistic activation of human immunodeficiency virus type 1 gene expression and virus multiplication.
... Our results are complementary to those of a number of recent experiments which have addressed the role of NF- k B transcription factors in HIV-1-regulated gene expression. Wes- tendorp et al. demonstrated ...
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Transcriptional and posttranscriptional regulation of macrophage specific colony stimulating factor gene expression by tumor necrosis factor Involvement of arachidonic acid metabolites
... monocytic differentiation. CSF-1 transcripts were undetectable in uninduced HL-60 cells, reached maximal levels by 3 h of exposure to TNF, and returned to that of control cells by 24 h. Transcriptional run-on analysis ...
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Expression of CD74 is increased in neurofibrillary tangles in Alzheimer's disease
... and tumor necrosis factor α, are increased in AD, along with gene expression of major histocompatibility complex (MHC) class II molecules and macrophage migration inhibitory ...
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Intravenous somatic gene transfer with antisense tissue factor restores blood flow by reducing tumor necrosis factor induced tissue factor expression and fibrin deposition in mouse meth A sarcoma
... tissue factor in endothelium. Although induction of tissue factor in cultured endothelial cells has been demon- strated by many groups, investigation of human atheroscle- rotic plaques and the vasculature ...
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Tumor necrosis factor alpha and epidermal growth factor act additively to inhibit matrix gene expression by chondrocyte
... The MAPK/ERK pathway plays an important role in direct- ing alterations of the cytoskeleton. For example, constitu- tively active MAPK induces morphological changes in fibroblasts, coinciding with disruption of stress ...
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Downregulation of protein disulfide isomerase in sepsis and its role in tumor necrosis factor alpha release
... α gene expression, ...α gene expression, the PCR was conducted at 30 cycles, each cycle consisting of 30 seconds at 94°C, 30 seconds at 60°C, and 1 minute at ...
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Tumor necrosis factor alpha gene and protein expression in adult feline myocardium after endotoxin administration
... TNF alpha mRNA and protein biosynthesis were examined in the adult feline heart after stimulation with endotoxin. When freshly isolated hearts were stimulated with endotoxin in vitro, de novo TNF alpha mRNA ...
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Tumor necrosis factor alpha negatively regulates hepatitis B virus gene expression in transgenic mice.
... These classes of inflammatory cells produce an assortment of cytokines, such as tumor necrosis factor alpha TNF-a, alpha interferon IFN-ot, gamma interferon IFN-y, interleukin-1 alpha IL[r] ...
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Early gestational mesenchymal stem cell secretome attenuates experimental bronchopulmonary dysplasia in part via exosome-associated factor TSG-6
... of tumor necrosis factor- α -stimulated gene-6 (TSG-6) in human BPD tracheal aspirate and lung tissue of the BPD mouse model and its detection in EXO fraction (A) Relative TSG-6 ...
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Indexing TNF-αgene expression using a gene-targeted reporter cell line
... Cell-based gene reporter assay systems were developed as an alternative system amenable to HTS over 10 years ago, and have been widely used to study transcription and gene ...defined gene promoters ...
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Targeting Mycobacterium tuberculosis Tumor Necrosis Factor Alpha Downregulating Genes for the Development of Antituberculous Vaccines
... the expression of the cyto- kine in infected macrophage ...the gene encoding fatty-acyl-CoA synthetase ...the gene encoding fatty acyl-CoA syn- thetase (rv3089) in in vitro systems support the ...
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Hippocampal gene expression dysregulation of Klotho, nuclear factor kappa B and tumor necrosis factor in temporal lobe epilepsy patients
... cytokines tumor necrosis factor ligand superfamily member 12 (TNFSF12, also known as TWEAK) and TNF downregulate KL in renal tubular cells through an NFkB-dependent mechanism mediated by histone ...
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Gene Expression of Tumor Necrosis Factor Alpha in Etanercept Treated Rheumatoid Arthritis Patients
... Distributing RA patients by their DAS-28 revealed that patients with a mod- erate activity (3.2 - 5.1) showed an increased expression compared to patients with low (<3.2) or high (>5.1) disease activity. No ...
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Regulation of murine type 1 plasminogen activator inhibitor gene expression in vivo Tissue specificity and induction by lipopolysaccharide, tumor necrosis factor alpha, and transforming growth factor beta
... (PAI-1) gene expression was studied in vivo employing a murine model ...growth factor-beta (TGF-beta) increased the steady-state levels of PAI-1 mRNA within 3 h in all tissues ...
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Human granulocyte macrophage colony stimulating factor induces expression of the tumor necrosis factor gene by the U937 cell line and by normal human monocytes
... Human granulocyte-macrophage colony-stimulating factor (GM-CSF) exerts profound effects on the proliferation, differentiation, and effector function of myeloid lineage cells. In contrast to its growth-promoting ...
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Tumor necrosis factor alpha inhibits albumin gene expression in a murine model of cachexia
... expression throughout the hepatic lobule. The mRNA levels of several other genes were unchanged. Hepatic nuclei from TNF alpha mice before the onset of weight loss were markedly less active in transcribing the ...
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The immediate early genes of human cytomegalovirus upregulate tumor necrosis factor alpha gene expression
... (IE) gene region are potent trans- activators, we used the monocyte cell line THP-1 and a transient transfection assay to determine if these viral proteins upregulate expression of the TNF ...TNF ...
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Cachectin/tumor necrosis factor regulates hepatic acute phase gene expression
... increased factor B gene expression, but had no effect on C2 gene expression, in murine fibroblasts transfected with cosmid DNA bearing the human C2 and factor B ...protein ...
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