Tumor spheroid
Drug delivery to solid tumors: the predictive value of the multicellular tumor spheroid model for nanomedicine screening
... multicellular tumor spheroid; MMP, matrix metalloproteinase; MT1, membrane type-1 metalloproteinase; mTHPC, meta-tetra(hydroxyphenyl)chlorine; Mv, membrane vesicle; NA, not applicable; NP, nanoparticle; ...
15
Applications of a new In vivo tumor spheroid based shell less chorioallantoic membrane 3 D model in bioengineering research
... Nanoparticles for drug delivery and treatment pur- poses could be introduced into the CAM vascular net- work for analysis and validation of its effectiveness to reach the tumor spheroid region penetrate the ...
7
Advances in establishment and analysis of three-dimensional tumor spheroid-based functional assays for target validation and drug evaluation
... influence tumor cell responses to microenvironmental ...breast tumor cells reverted their morphological and functional features to a normal epithelial phenotype, but only in three dimen- sions ...vivo ...
21
Inhibition of hexokinase-2 with targeted liposomal 3-bromopyruvate in an ovarian tumor spheroid model of aerobic glycolysis
... Lastly, in order to investigate whether 3D arrangement of cells physically prevents drug penetration and whether upregulation of glycolytic activity in spheroids leads to fur- ther resistance to 3-BPA in terms of ...
19
GSK-3β regulates the endothelial-to-mesenchymal transition via reciprocal crosstalk between NSCLC cells and HUVECs in multicellular tumor spheroid models
... Lung cancer continues to be the most common cause of cancer-related mortality worldwide. Between 52 and 58% of lung cancer patients present with advanced-stage dis- ease, and a vast majority of these patients do not ...
14
Microfluidic device for primary tumor spheroid isolation
... the spheroid isolation units ...the spheroid isolation units contained trapped ...500 spheroid isolation units. Spheroid isolation efficiency of the chip depends on many facts, such as the ...
7
Three-Dimensional Quantification of Spheroid Degradation-Dependent Invasion and Invadopodia Formation
... available tumor spheroid model that allows long-term high-resolution imaging of the tumor, precise quantification, and visualization of matrix degradation and multichannel ...the tumor ...
11
Analysis of Oxygen Concentration Equation in Multi Cell Tumour Spheroid
... The Oxygen concentration at the centre is dependent on the outer radius of the sphere. The behaviour of the equation can be seen in the graph (Figure 7). In the diagrammatic representation of three layer structure of ...
7
MicroRNA profiling of a CD133+spheroid-forming subpopulation of the OVCAR3 human ovarian cancer cell line
... and tumor spheroid formation was noted in OVCAR3, TOV112D, and SKOV3 (Figure ...make tumor spheroids in the non-adherent culture ...to tumor sphere formation. Tumor spheres tend to grow ...
9
FAK activity protects nucleostemin in facilitating breast cancer spheroid and tumor growth
... anchorage-independent spheroid) and as orthotopic ...promoted tumor spheroid growth, stabilized NS levels, and promoted pS65 4E-BP1 phosphorylation in the presence of inhibited ...
17
3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture
... Background: Three dimensional (3D) cell cultures have been an area of increasing interest and relevance across several research fields including drug discovery, developmental biology and stem cell-based therapies. ...
10
Forward and Backward Waves in High-Frequency Diffraction by an Elongated Spheroid
... The asymptotics of [10] is derived under the assumptions that the body is strongly elongated, that is the parameter χ introduced in the introduction is of order one, that the body has rotational symmetry and the incident ...
23
A cellular automaton model for spheroid response to radiation and hyperthermia treatments.
... the spheroid, whereas the analytical model assumes a perfect ...for spheroid production and used spheroids 4 to 17 days after formation for their analysis and parameter ...of spheroid sizes obtained ...
12
Computational and Experimental Investigation of Flow Around a 3 1 Prolate Spheroid
... The spheroid, sting, foil support and upper limb of the tunnel was modelled using a hybrid mesh with a predominance of hexahedral ...The spheroid, sting and foil support where surrounded with an offset ...
7
Solutions of the Sitnikov's circular restricted three body problems when primaries are oblate spheroid
... are spheroid in shape. But if the primaries are oblate spheroid in shape then due to their oblateness the primaries will not be equidistant from their center of ...
8
Stability of the Sitnikov's circular restricted three body problem when the primaries are oblate spheroid
... are spheroid in shape. But if the primaries are oblate spheroid in shape then due to their oblateness the primaries will not be equidistant from their center of ...
8
Elucidating the Secretome of Lung Spheroid Cells using LC/MS/MS.
... In this study, proteomic analysis of conditioned media from cultured LSC, isolated from three healthy human donor lung tissues, was analyzed using mass spectrometry to iden[r] ...
106
Preclinical Study of Lung Spheroid Cell Therapy in Rodent Models of Pulmonary Fibrosis.
... equal volume of Iscove’s modified Dulbecco’s medium (IMDM; Life Technologies) containing 20% fetal bovine serum (FBS; Corning Life Sciences, Acton, MA, http://www.corning.com/). Subsequently, the tissue samples were ...
248
High throughput spheroid screens using volume, resazurin reduction and acid phosphatase activity
... in-house, the assay is thousands of times cheaper than the ATP-based kits. The resazurin assay does not kill or lyse the spheroids and the latter can be harvested for dissociation and cell counts, flow cytometry or ...
23
Therapeutic Potential of Lung Spheroid Cell-Secreted Factors in Rodent Models of Pulmonary Fibrosis.
... As an important in vitro potency indicator of cell therapy, we performed an endothelial tube formation assay to demonstrate the therapeutic potential of WL-LSCs and TB-LSCs in comparison to MSCs (Fig. 4). We showed that ...
134