type 1 protein phosphatase
Vimentin dephosphorylation at ser-56 is regulated by type 1 protein phosphatase in smooth muscle
... this report, we also observe that treatment with okadaic acid increases vimentin phosphorylation at Ser-56 in smooth muscle. Because the inhibitors are not specific, we also used lentivirus-mediated shRNA against PP1to ...
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Genetic Interactions Between REG1/HEX2 and GLC7, the Gene Encoding the Protein Phosphatase Type 1 Catalytic Subunit in Saccharomyces cerevisiae
... Mutations in GLC7, the gene encoding the type 1 protein phosphatase catalytic subunit, cause a variety of abberrant phenotypes in yeast, such as impaired glycogen synthes[r] ...
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Density-enhanced protein tyrosine phosphatase 1 (DEP-1) regulation of epithelial cell adhesions
... cell type differences may be critical, especially given that SV40Tag- transformed cells elevate expression of PTP1B ...regulatory protein such as ...
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Inducible expression of catalytically active type 1 serine/threonine protein phosphatase in a human carcinoma cell line
... cent protein coding sequence under control of a bi-direc- tional tetracycline response ...at 1 ug/ml final ...rtTA-nls protein and transient transfection with ...at 1 ug/ml for ...
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Serine/Threonine Protein Phosphatase Mediated Control of the Peptidoglycan Cross Linking l,d Transpeptidase Pathway in Enterococcus faecium
... reduced phosphatase activity, but not in the parental strain D344S, which harbors a wild-type copy of stpA ...impaired phosphatase activity were both required for ampicillin ...the phosphatase ...
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Protein Phosphatase 2A Enhances Activation of Human Immunodeficiency Virus Type 1 by Phorbol Myristate Acetate
... the phosphatase is preferable, but this approach has the potential to be complicated by the trans- lational and posttranslational autoregulatory mechanisms, seen in various cell lines, that serve to maintain ...
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Protein Phosphatase Type 1-Interacting Protein Ysw1 Is Involved in Proper Septin Organization and Prospore Membrane Formation during Sporulation
... two protein complexes are associated with the prospore membrane as it ...edge protein complex, which exists at the lip of the prospore membranes and consists of three com- ponents: Ssp1, Ady3, and Don1 (27, ...
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Protein Phosphatase Type 1 Regulates Ion Homeostasis in Saccharomyces cerevisiae
... Protein phosphatase type 1 (PP1) is encoded by the essential gene GLC7 in Saccharomyces cerevisiae. glc7- 109 (K259A, R260A) has a dominant, hyperglycogen defect and a recessive, ion and drug ...
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Plasmodium falciparumencodes a conserved active inhibitor-2 for Protein Phosphatase type 1: perspectives for novel anti-plasmodial therapy
... a protein as a PP1c regulator ...I2 protein identified so far (144 amino acids for PfI2 versus 164–205 for other I2), the absence of one binding site (KGILK) and the fundamental difference in the RVxF motif ...
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Evidence that homozygous PTPRD gene microdeletion causes trigonocephaly, hearing loss, and intellectual disability
... Receptor type tyrosine protein phosphatase delta; ID: intellectual disability; MRI: Brain magnetic resonance imaging; CMA: Chromosomal microarray analysis; ChAS: Affymetrix Chromosome Analysis Suite ...
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Direct interaction between the catalytic subunit of Protein Phosphatase 1 and pRb
... We next sought to determine whether the interaction between PP1 and pRb involved a PP1-trageting subunit [1]. For this purpose we used a full-length GST-Rb affinity column to capture pRb-directed PP1 complexes ...
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PTEN dephosphorylates AKT to prevent the expression of GLUT1 on plasmamembrane and to limit glucose consumption in cancer cells
... wild type exon 5 (represented at very low level), thus confirming that OVCAR-3 cells express the PTEN allele with the missense Y155C mutation (Figure ...the phosphatase domain (Figure 4B), and it has been ...
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Ectopic Expression of Inhibitors of Protein Phosphatase Type 1 (PP1) Can Be Used to Analyze Roles of PP1 in Drosophila Development
... Wings from vg-GAL4 UAS tkv (vg⬎tkv) flies were essen- tially wild type (Figure 3D). When we tested UAS-tkv in combination with vg⬎punt, we found that extra tkv dominantly enhanced the effect of punt and phenotypi- ...
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Deficiency in phosphorylase phosphatase activity despite elevated protein phosphatase type 1 catalytic subunit in skeletal muscle from insulin resistant subjects
... by protein phosphatase type-1 ...total phosphatase activity. Spontaneous and total PP-1 activities were reduced in insulin resistant subjects (P less than ...trypsin-labile ...
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Anti-CD45RO Suppresses Human Immunodeficiency Virus Type 1 Replication in Microglia: Role of Hck Tyrosine Kinase and Implications for AIDS Dementia
... were prepared from day 7 HIV-1-infected microglial cell cultures treated with ␣ CD45RO or control IgG2a and immunoprecipitated with total ␣ -Hck antibody. Western blot analysis was then performed with ␣ -pSrc ...
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A Toxoplasma gondii Leucine-Rich Repeat Protein Binds Phosphatase Type 1 Protein and Negatively Regulates Its Activity
... with 1% SDS, 50 mM sodium acetate (pH ...of 1 l of Clontech Taq mixture DNA polymerase (Clontech) in 50-l reaction mixture ...for 1 min and 30 s (2); elongation for 1 min and 30 seconds at ...
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Catalytic Subunit 1 of Protein Phosphatase 2A Is a Subunit of the STRIPAK Complex and Governs Fungal Sexual Development
... striatin-interacting phosphatase and kinase (STRIPAK) complex, which has been described in organisms ranging from yeasts to ...heterotrimeric protein phosphatase PP2A is a subunit of ...subunit ...
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Alanine-Scanning Mutagenesis of Protein Phosphatase Type 1 in the Yeast Saccharomyces cerevisiae
... If most of the defects caused by the alanine-scanning alleles result from im- paired interactions between the mutant products and Glc7p binding proteins, then the fact[r] ...
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Characterization of Farnesylated Protein Tyrosine Phosphatase TcPRL-1 from Trypanosoma cruzi
... major protein band, with an apparent molecular mass of ⬃ 47 kDa, was detected in the transfected parasite population with anti-TcPRL-1 ...(GFP–TcPRL- 1 ⌬ CAVM or GFP–TcPRL-1C107S ⌬ CAVM) is in ...
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2C Proteins of Enteroviruses Suppress IKKβ Phosphorylation by Recruiting Protein Phosphatase 1
... viral protein in human enteroviruses, we inquired whether the 2C proteins of other enteroviruses also regulate the NF- B signaling pathway via inhibition of IKK  phosphoryla- ...Through protein sequence ...
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