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Type 2 Secretion System

Structural Basis of Type 2 Secretion System Engagement between the Inner and Outer Bacterial Membranes

Structural Basis of Type 2 Secretion System Engagement between the Inner and Outer Bacterial Membranes

... protein secretion. In Gram-negative bacteria, the type 2 secretion system (T2SS) is composed of a pseudopilus assembly platform in the inner membrane and a secretin complex in the outer ...

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Clinical impact of the type VI secretion system on virulence of Campylobacter species during infection

Clinical impact of the type VI secretion system on virulence of Campylobacter species during infection

... The clinical characteristics of infection with Campylobac- ter spp. in the study population are similar to those previ- ously described by various authors [1, 6]. Infection with C. jejuni and C. coli were clinically ...

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Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system

Legionella DotM structure reveals a role in effector recruiting to the Type 4B secretion system

... IcmSW-independent secretion mechanism 27 ...(Table 2) was chosen: the sequence of OSM is a single-residue substitution (L to E at the very C terminus) of the OSS peptide described in Lifshitz et ...

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Escherichia coli type III secretion system 2: a new kind of T3SS?

Escherichia coli type III secretion system 2: a new kind of T3SS?

... III secretion systems (T3SSs) are employed by Gram-negative bacteria to deliver effector proteins into the cytoplasm of infected host ...second type III secretion system, the E. coli ...

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The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli

The CpxR regulates type VI secretion system 2 expression and facilitates the interbacterial competition activity and virulence of avian pathogenic Escherichia coli

... Deletion of cpxR attenuates APEC virulence in ducks The Cpx TCS has varying effects on the pathogenesis of different bacteria [26–35, 41, 44, 52, 53]. In APEC, the Cpx system has been implicated in the regulation ...

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The Ess/Type VII secretion system of Staphylococcus aureus shows unexpected genetic diversity

The Ess/Type VII secretion system of Staphylococcus aureus shows unexpected genetic diversity

... Background: Type VII protein secretion (T7SS) is a specialised system for excreting extracellular proteins across bacterial cell membranes and has been associated with virulence in Staphylococcus ...

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Selective Purification of Recombinant Neuroactive Peptides Using the Flagellar Type III Secretion System

Selective Purification of Recombinant Neuroactive Peptides Using the Flagellar Type III Secretion System

... flagellar secretion apparatus (indicated by an orange asterisk in the figure) from early to late substrate ...in secretion of FlgM and substrates needed during the final phase of flagellum ...and ...

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In Situ Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System

In Situ Molecular Architecture of the Helicobacter pylori Cag Type IV Secretion System

... Cag secretion machines (400 ⫻ 400 ⫻ 400 voxels) were visually identified and then extracted from 1,924 cryo-tomographic reconstruc- ...Cag secretion machine were estimated based on the orientation of each ...

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Structure of a bacterial type III secretion system
in contact with a host membrane in situ

Structure of a bacterial type III secretion system in contact with a host membrane in situ

... Figure 2 | In situ structure of host-contact Chlamydia ...a type III secretion system (a) and within a vacuole of a U2OS cell ...with type III secretion systems (white ...

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X ray crystal structures of the Type IVb Secretion System DotB ATPases

X ray crystal structures of the Type IVb Secretion System DotB ATPases

... Proteins were diluted to the desired concentration in the gel filtration buffer supplemented with 2 mM MgCl2 before the assay. Assays were conducted using the ATPase/GTPase Assay Kit (Sigma Aldrich) according to ...

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A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins

A Novel Mechanism for Protein Delivery by the Type 3 Secretion System for Extracellularly Secreted Proteins

... 1, 2, or 3 h (to allow the injection of translocator and effector proteins into or onto the host cells) and then these bacteria are eliminated with tetracycline followed by incubation of these epithelial cells ...

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Characterization of the mode of action of Aurodox, a type III secretion system inhibitor from streptomyces goldiniensis

Characterization of the mode of action of Aurodox, a type III secretion system inhibitor from streptomyces goldiniensis

... 500 ␮l of fresh MEM-HEPES (with or without 5 ␮g/ml of Aurodox) was added, in addition to 15 ␮l of bacterial culture. The plate was then centrifuged at 250 ⫻ g for 3 min and was left to incubate initially for 1 h. Serial ...

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Interactions of the Transmembrane Polymeric Rings of the Salmonella enterica Serovar Typhimurium Type III Secretion System

Interactions of the Transmembrane Polymeric Rings of the Salmonella enterica Serovar Typhimurium Type III Secretion System

... The Salmonella enterica serovar Typhimurium and Shigella flexneri NCs have been purified and examined at approximately 17-Å resolution by cryoelectron microscopy (cryo-EM) with three-dimensional reconstructions, ...

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The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly in Acinetobacter baumannii

The Tip of the VgrG Spike Is Essential to Functional Type VI Secretion System Assembly in Acinetobacter baumannii

... Taken together, our results show that VgrGi can inhibit the T6SS of its native strain, as well as that of Ab17978 mutant strains with limited VgrGs. Given that AbCAN2 and Ab17978 possess a different arsenal of effectors, ...

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Optimization of a Type III Secretion System-Based Pseudomonas aeruginosa Live Vector for Antigen Delivery

Optimization of a Type III Secretion System-Based Pseudomonas aeruginosa Live Vector for Antigen Delivery

... QS system inactivation, previous experiments showed that it conferred a reduced virulence in various mod- els, in accordance with the role of QS signaling of many viru- lence factors of ...S54-Ova secretion ...

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Analysis of Vibrio cholerae genomes identifies new type VI secretion system gene clusters

Analysis of Vibrio cholerae genomes identifies new type VI secretion system gene clusters

... To determine whether TleV1 is toxic to cells in a manner consistent with a lipase, we examined killing induced by TleV1 using confocal microscopy (Nikon A1plus). Propidium iodide, which stains the DNA of dead cells with ...

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Translocation through the Conjugative Type IV Secretion System requires unfolding of its protein substrate

Translocation through the Conjugative Type IV Secretion System requires unfolding of its protein substrate

... placing the flask into a 42°C shaking water bath for 15 min. The flask was then cooled in the ice-water slurry, and the electrocompetent cells were prepared by washing the cells twice with 40 ml of ice-cold distilled water ...

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Critical role of a sheath phosphorylation site on the assembly and
function of an atypical type VI secretion system

Critical role of a sheath phosphorylation site on the assembly and function of an atypical type VI secretion system

... (Fsp_TX077308) exhibited only 48% amino acid identity with the 31 other IglB proteins whereas they both share 85% identity. Excluding these 2 outliers, among 506 sites, 458 were without polymorphism (91%), with ...

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“Candidatus Paraporphyromonas polyenzymogenes” encodes multi-modular cellulases linked to the type IX secretion system

“Candidatus Paraporphyromonas polyenzymogenes” encodes multi-modular cellulases linked to the type IX secretion system

... Fig. 2 Selected metabolic features of “ Candidatus Paraporphyromonas polyenzymogenes ” (AGa) as inferred from genome and proteome ...file 2: Table ...file 2: Table S1, Additional file 7: Table ...

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Piericidin A1 Blocks Yersinia Ysc Type III Secretion System Needle Assembly

Piericidin A1 Blocks Yersinia Ysc Type III Secretion System Needle Assembly

... of type III secretion is reversible, and this reversibility is independent of new protein ...Yop secretion is ...for 2 h under T3SS-inducing conditions, washed the bacteria to remove the ...

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