type I and P pilus
Attenuation of the Type IV Pilus Retraction Motor Influences Neisseria gonorrhoeae Social and Infection Behavior
... communication. One possible explanation is that it is indirectly involved in interbacterial mechanical sensing and signaling. The Pseudomonas aeruginosa Tfp biogenesis protein PilY1 is a mecha- nosensor that regulates ...
11
Ordered and ushered; the assembly and translocation of the adhesive Type I and P Pili
... Upon translocation to the external milieu, the pilus must fulfil its adhesive function. The Fim and Pap pili are examples of monoadhesive pili, with adhesion being mediated entirely by the terminal adhesin ...
21
Identification of Enterotoxigenic Escherichia coli Harboring Longus Type IV Pilus Gene by DNA Amplification
... longus type IV pilus produced by human enterotoxigenic Escherichia coli (ETEC) was achieved by the use of specific oligonucleotide primers designed from the nucle- otide sequence of ...a type IV ...
5
The Enterococcus faecalis EbpA Pilus Protein: Attenuation of Expression, Biofilm Formation, and Adherence to Fibrinogen Start with the Rare Initiation Codon ATT
... -Galactosidase production is reduced by the presence of ATT as the start codon of an ebpA-lacZ fusion. To explore the direct contribution of the EbpA initiation codon to translational efficiency, two translational ...
9
The remarkable biomechanical properties of the Type 1 Chaperone Usher Pilus: a structural and molecular perspective
... that type 1 pilus rods require slightly higher forces to induce rod unwinding than do P pilus rods, prompting the suggestion that type 1 pili are better adapted to withstand the more ...
11
Structural and molecular biology of a protein polymerizing nanomachine for pilus biogenesis
... (iii) type IV pili, (iv) type V pili, and (v) type IV secretion pili ...elaborated pilus biogenesis mechanisms: the machineries responsible for pro- ducing type IV pili and type ...
14
Antibodies against the Majority Subunit (PilA) of the Type IV Pilus of Nontypeable Haemophilus influenzae Disperse Moraxella catarrhalis from a Dual Species Biofilm
... There is a growing body of evidence that bacteria newly released from a biofilm are transcriptionally and phenotypically distinct from either biofilm-resident or planktoni- cally grown bacteria (36–39). Several reports ...
18
Suppression of type 1 pilus assembly in uropathogenic Escherichia coli by chemical inhibition of subunit polymerization
... for type 1 pilus DSE inhibitors we employed an SDS– PAGE- based assay that monitored the formation of an SDS-stable complex between FimH and the FimG Nte (Figure 2a; see the Mate- rials and methods ...
11
Type IV secretion in Gram negative and Gram positive bacteria
... the I-layer (Rivera-Calzada et al., 2013). The I-layer in the arrangement is composed of 14 VirB9 N-terminal domains and covers the outer ...and I-layer ...blocked pilus biogenesis, but ...
50
Extracellular DNA and Type IV Pilus Expression Regulate the Structure and Kinetics of Biofilm Formation by Nontypeable Haemophilus influenzae
... ⬇ 1. In addition, the simulations under the above condition produced smoother biofilm surfaces (Fig. 6A) with smaller coefficients of variation than with the biofilms formed in silico by the wild-type NTHI. We ...
19
Genome characterization of bile-isolated Shewanella algae ACCC
... factor database. Comparative analysis of ACCC, clinical isolated MARS 14, and environmental isolated C6G2 revealed strain specific genes mshB which is associated with MSHA type IV pilus biogenesis. S. algae ...
6
Acquisition and Exclusion of Pathogenicity Island 1 (PAPI-1) in Pseudomonas Aeruginosa
... to type IV secretion systems (T4SSs) ...conjugative pilus were found in plasmid R64, except PilD peptidase, responsible for the cleavage of the PilS2 leader peptide, located in the chromosome ...
162
Haloferax Volcanii Strategies to Regulate Type IV Pilus Dependent Adhesion and Microcolony Formation
... use type IV pili to attach to abiotic surfaces and form microcolonies (O'Toole & Kolter, 1998, Ghosh & Albers, ...and type IV pili and the respective sets of genes that encode components of the ...
199
The Type IVa Pilus Machinery Is Recruited to Sites of Future Cell Division
... Cowles et al. (29) reported that both the flagellum and T4aP were mislocalized in P. aeruginosa mutants lacking the polar organelle coordinating (Poc) proteins, but the mechanism remains enigmatic. The T4aP ...
18
Type IV Pilus Secretins Have Extracellular C Termini
... catalyst is immobilized on glass tubes, and the reactivity of the short-lived iodous (I ⫹ ) intermediate is limited by diffusion. Four residues (Y215, Y315, Y360, and Y503) were iodinated ⱖ 5% of the time and were ...
13
Structure of a Chaperone Usher Pilus reveals the molecular basis of rod uncoiling
... PapA pilus rods (wild-type and AzF-incorporated mutants) were labeled with click-it Alexa Fluor 647 DIBO alkyne (Life Technologies) to assess the accessibility of each chosen site of AzF incorporation in ...
12
Adhesive threads of extraintestinal pathogenic Escherichia coli
... the type 1 fimbriae was iden- tified, characterized and purified in 1988 and this protein was found to be antigenically conserved among strains with different pilin serotypes, and located at the pilus tip ...
12
The Cryoelectron Microscopy structure of the Type 1 Chaperone Usher Pilus Rod
... the type 1 pilus rod allows us to fully appreciate the similarities and differences of the two archetypal chaperone-usher pili of ...the pilus rod, also shares several similarities. Both the ...
16
Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry
... new pilus sequences and preventing mutations from ablating the ability of a pathogenic bacterium utilizing the chaperone-usher system to export these essential virulence ...
25
Large thermoelectric power factors and impact of texturing on the thermal conductivity in polycrystalline SnSe
... which is one of the highest zT values observed for polycrystalline samples due to an optimised p- type carrier concentration. 21 Because of the anisotropic crystal structure, texturing of polycrystalline ...
22