Type X collagen
Type X collagen levels are elevated in serum from human osteoarthritis patients and associated with biomarkers of cartilage degradation and inflammation
... Further characterization of ColX monoclonal antibody, NB509-11G8, was conducted by western blotting with cell lysates of human osteosarcoma cell line, U2-OS ex- pressing type X collagen. The cell ...
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A nonsense mutation in the carboxyl terminal domain of type X collagen causes haploinsufficiency in schmid metaphyseal chondrodysplasia
... for type X collagen, the only approach likely to resolve the issue is to undertake direct analysis of type X collagen expression in the growth plate cartilage of SMCD pa- ...
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Effect of nitrogen-rich cell culture surfaces on type X collagen expression by bovine growth plate chondrocytes
... express type X collagen) on PPE:N, Primaria ® , or regular polystyrene (PS) culture dishes was investigated using reverse transcriptase (RT) and polymerase chain reaction ...of type X ...
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Differential expression of type X collagen in a mechanically active 3-D chondrocyte culture system: a quantitative study
... of type X collagen synthesis, similar to its activation in the hypertrophic ...Col X gene expression is the same between growth plate chondrocytes and the osteoarthritic ...
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Complete degradation of type X collagen requires the combined action of interstitial collagenase and osteoclast derived cathepsin B
... 59-kD type X molecule at 37 degrees C, but only the 32-kD collagenase-generated ...32-kD type X collagen fragment by osteoclast lysates exhibited the following properties: (a) cleavage ...
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Hypertrophy is not a prerequisite for type X collagen expression or mineralization of chondrocytes derived from cultured chick mandibular ectomesenchyme
... Int / De, BinI JX 6RJ 69 j(199 j) 6R3 Original Arlie/I' Hypertrophy is not a prerequisite for type X collagen expression or mineralization of chondrocytes derived from cultured chick mandibular ectome[.] ...
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Endochondral bone formation in toothless (osteopetrotic) rats: failures of chondrocyte patterning and type X collagen expression
... Col X expression also fails in this region of the mutant growth ...Col X and mineralization by these ...Col X expression and mineral- ization of cartilage by hypertrophied chondrocytes in the lateral ...
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Human osteochondritis dissecans fragment derived chondrocyte characteristics ex vivo, after monolayer expansion induced de differentiation, and after re differentiation in alginate bead culture
... and type II collagen mRNA expression levels and decreases in the type I collagen mRNA expression and the type I to II collagen expression ratio, the resulting mRNA expression ...
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Differential effects of ERK and p38 signaling in BMP-2 stimulated hypertrophy of cultured chick sternal chondrocytes
... of type II and IX collagens, and the emergence of type X collagen (Col X), which is a specific marker of hypertrophy ...in type X colla- gen expression appeared to ...
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Disrupting the Indian hedgehog signaling pathway in vivo attenuates surgically induced osteoarthritis progression in Col2a1 CreERT2; Ihhfl/fl mice
... Gli2, type X collagen (the typical hypertrophic chondrocyte marker) and other cartilage-degrading enzymes, such as MMPs and ca- thepsins are effectively downregulated by Ihh deletion (Figures 3, 5 ...
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Establishment of rat ankle post traumatic osteoarthritis model induced by malleolus fracture
... II collagen, type X collagen, and MMP-13 were ana- lyzed by immunohistochemistry using an UltraSensitive™ SP IHC Kit (Maixin Biotech, Fuzhou, ...rat type II collagen (Boster, ...
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Differential effects of ERK and p38 signaling in BMP-2 stimulated hypertrophy of cultured chick sternal chondrocytes
... of type II and IX collagens, and the emergence of type X collagen (Col X), which is a specific marker of hypertrophy ...in type X colla- gen expression appeared to ...
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Effects of 5 Aza 2’ Deoxycytidine on Proliferation and Differentiation of Embryonic Chick Caudal Region Chondrocytes in Culture
... type X collagen is accompanied by a relative decrease in other collagens [7] suggesting the change in the extracellular ...specific collagen (Type II) as the main biosynthetic product ...
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GPR120 is an important inflammatory regulator in the development of osteoarthritis
... Col X: Type X collagen; Cox2: Cyclooxygenase 2; DHA: Docosahexaenoic acid; ELISA: Enzyme- linked immunosorbent assay; FA: Fatty acid; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; GPR120: ...
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Biomarkers of collagen synthesis predict progression in the PROFILE idiopathic pulmonary fibrosis cohort
... Alongside collagen type-3, our data suggest that changes in collagen type-6 are also informative of dis- ease behaviour in IPF and may be an important driver of ...fibrosis. Collagen ...
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A program of cell death and extracellular matrix degradation is activated in the amnion before the onset of labor
... amnion type I collagen matrix is degraded with the accumulation of three-quarter length type I collagen fragments in extraembryonic fluid, characteristic of the cleavage of fibrillar ...
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Identification of type IV collagen exposure as a molecular imaging target for early detection of thoracic aortic dissection
... Col-IV: type IV collagen; Col-I: type I collagen; Col-III: type III collagen; CDR: Col-IV-DOTA-Gd-rhodamine B; MRI: magnetic resonance imaging; NSE: normalized signal ...
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Preparation and Characterisation of Collagen from Freshwater Fish Scales
... muscle type I collagen, porcine dermis type I collagen and other seawater fish collagens, freshwater carp fish scales collagen contained relative high half-cystine (Cys-s), but lower ...
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Collagen types I and II distribution: a relevant indicator for the functional properties of articular cartilage in immobilised and remobilised rabbit knee joints
... in collagen type I and type II distribution in the articular cartilage of immobilised and remobilised rabbit knee ...3. Collagen type I and type II in the articular cartilage of ...
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CK2.1, a bone morphogenetic protein receptor type Ia mimetic peptide, repairs cartilage in mice with destabilized medial meniscus
... of collagen type II and type IX in ...gen type IX and XI are necessary to form covalent cross fibrillation to provide the tensile strength [27, ...of collagen type IX ...
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