ubiquitin-specific protease 15
Ubiquitin-specific protease 11 functions as a tumor suppressor by modulating Mgl-1 protein to regulate cancer cell growth
... RanBPM elongates the half-life of Mgl-1 through the inhibition of ubiquitination for Mgl-1 and inhibition of RanBPM expression leads to decreased level of Mgl-1 [15]. In addition, it interacts with USP11 which is ...
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Ubiquitin-specific protease 7 is a drug-able target that promotes hepatocellular carcinoma and chemoresistance
... Place the cells into a 6-well plate at the appropriate den- sity. After the cells adhere to the cells within 24 h, trans- fection is performed. Lipofectamine 2000 was diluted in DMEM medium at various concentrations to ...
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Ubiquitin-specific protease 3 promotes cell migration and invasion by interacting with and deubiquitinating SUZ12 in gastric cancer
... SUZ12 (Suppressor of zeste 12 protein homolog) is a core component of the Polycomb PRC2-HMTase complex, which has been shown to be involved in stem cell mainten- ance and development [11, 12]. SUZ12 is upregulated in ...
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<p>Ubiquitin-specific protease 4 promotes glioblastoma multiforme via activating ERK pathway</p>
... Protein of tissues and GBM cell lines was extracted using RIPA lysis buffer (Solarbio, Beijing, People’s Republic of China) containing protease inhibitors. Protein concentration was measured using bicinchoninic ...
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A Gammaherpesvirus Ubiquitin-Specific Protease Is Involved in the Establishment of Murine Gammaherpesvirus 68 Infection
... Cell preparation and surface marker staining. Single-cell suspensions were prepared from spleens by mechanical disruption in ice-cold RPMI 1640 medium (Invitrogen Life Technologies, Carlsbad, CA) containing 10% fetal ...
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Targeting ubiquitin-specific protease 22 suppresses growth and metastasis of anaplastic thyroid carcinoma
... Immunolohistochemistry and staining evaluation All the tissue specimens were fixed with PBS- buffered 10% formalin overnight, embedded with paraffin, non-serially sectioned (4 µm), and mounted on the polylysine-covered ...
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Mutagenesis of the Active-Site Cysteine in the Ubiquitin-Specific Protease Contained in Large Tegument Protein pUL36 of Pseudorabies Virus Impairs Viral Replication In Vitro and Neuroinvasion In Vivo
... Herpesviruses possess a large double-stranded DNA ge- nome, comprising more than 70 open reading frames (ORFs) encoding viral proteins, of which at least 30 are incorporated into the mature virus particle. The most ...
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Negative regulation of NF-κB signaling in T lymphocytes by the ubiquitin-specific protease USP34
... In addition to NF-κB, TCR ligation kindles various signal- ing pathways including Nuclear factor of activated T-cells (NFAT) and the Mitogen-activated protein kinase (MAPK) Extracellular signal-regulated kinases (ERK) ...
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Prevalence of Plasmodium falciparum delayed clearance associated polymorphisms in adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1) genes in Ghanaian isolates
... For the pfubp1 gene, 81 quality sequence data were analysed comprising 37% (30/81), 35.8% (29/81) and 27.2% (22/81) from Cape Coast, Begoro and Navrongo, respectively. About 11.1% (9/81) of the isolates had no mutations ...
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Autocatalytic Activity of the Ubiquitin-Specific Protease Domain of Herpes Simplex Virus 1 VP1-2
... An N-terminal variant whose abundance is dependent upon functional USP activity. We next examined a series of N-ter- minal variants (Fig. 1a) which included progressively more of the protein, in particular extending ...
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Assess the expression of ubiquitin specific protease USP2a for bladder cancer diagnosis
... All primary tumor samples from patients who under- went TUR or radical cystectomy were histologically veri- fied as urothelial carcinoma at Chungbuk National University in South Korea [18, 19]. Non-cancerous sur- ...
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Ubiquitin specific protease 19 involved in transcriptional repression of retinoic acid receptor by stabilizing CORO2A
... that ubiquitin specific protease 19 (USP19) as a DUB is gradually decreased during adipogenesis and it regulates coronin 2A (CORO2A) as one of the components for the nuclear receptor co-repressor ...
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Prognostic and clinicopathological significance of ubiquitin-specific protease 22 overexpression in cancers: evidence from a meta-analysis
... Seven of the studies were of patients with digestive system cancers (colorectal cancer, hepatocellular cancer, esophageal cancer, gastric cancer, and pancreatic cancer), 16,18,23,28–31[r] ...
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Ubiquitin-specific protease 14 regulates c-Jun N-terminal kinase signaling at the neuromuscular junction
... Background: Ubiquitin-specific protease 14 (USP14) is one of three proteasome-associated deubiquitinating enzymes that remove ubiquitin from proteasomal substrates prior to their ...
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The Ubiquitin-Specific Protease USP7 Modulates the Replication of Kaposi's Sarcoma-Associated Herpesvirus Latent Episomal DNA
... Kaposi’s sarcoma herpesvirus (KSHV) belongs to the gamma-2 Herpesviridae and is associated with three neoplastic disorders: Kaposi’s sarcoma (KS), primary effusion lymphoma (PEL), and multicentric Castleman’s disease ...
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Reciprocal Activities between Herpes Simplex Virus Type 1 Regulatory Protein ICP0, a Ubiquitin E3 Ligase, and Ubiquitin-Specific Protease USP7
... An issue that must be considered arises from reports of a second E3 ubiquitin ligase activity of ICP0, distinct from that of the RING finger, conferred by the region of ICP0 that includes the USP7 binding domain ...
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Ubiquitin-specific protease 7 expression is a prognostic factor in epithelial ovarian cancer and correlates with lymph node metastasis
... Total cell extracts were prepared with NP-40 Buffer (50 mM Tris–HCl pH 7.5, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate) and protease inhibitor cocktails (1:1,000). Approximately 40 μ g of each protein sample ...
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Overview of the organization of protease genes in the genome of Leishmania spp
... of protease genes in four species, a method- ology we applied to identify species-specific features that may account for phenotypic or virulence differences among the studied ...of protease genes ...
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Ubiquitin-specific protease 12 interacting partners Uaf-1 and WDR20 are potential therapeutic targets in prostate cancer
... Recently, ML323 and its derivatives were identified as more potent and specific Usp1/Uaf-1 inhibitors. Authors suggested that ML323 specificity was achieved by targeting the DUB complex as opposed to the Usp1 ...
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The Yeast Ubiquitin Protease, Ubp3p, Promotes Protein Stability
... Stu1p is a microtubule-associated protein required for spindle assembly. In this article we show that the temperature-sensitive stu1-5 allele is synthetically lethal in combination with ubp3, gim1-gim5, and kem1 ...
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