Ubiquitin specific protease 4 (USP4)
Tricho rhino phalangeal syndrome 1 protein functions as a scaffold required for ubiquitin specific protease 4 directed histone deacetylase 2 de ubiquitination and tumor growth
... recruits USP4 to stabilize HDAC2 repressing the expres- sion of AES, Casp7, PERP , and ZW10 to confer tumor ...recruits USP4 to stabilize HDAC2 and represses expression of AES, Casp7, PERP , and ZW10 to ...
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<p>Ubiquitin-specific protease 4 promotes glioblastoma multiforme via activating ERK pathway</p>
... of USP4. Of note, USP4 overexpression can only overcome partial apoptosis induced by ...by USP4 or p-ERK1/2, we performed time-course experiment with/without U0126 ...plus USP4 ectopic ...
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Structure and catalytic regulatory function of ubiquitin specific protease 11 N terminal and ubiquitin like domains
... for ubiquitin speci fi c proteases to be able to tightly regulate their catalytic activity as they occupy key positions in important signaling ...(Figures 4 and 5), and solved the crystal structures of the ...
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Targeting ubiquitin-specific protease 22 suppresses growth and metastasis of anaplastic thyroid carcinoma
... Immunolohistochemistry and staining evaluation All the tissue specimens were fixed with PBS- buffered 10% formalin overnight, embedded with paraffin, non-serially sectioned (4 µm), and mounted on the ...
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The Ubiquitin-Specific Protease USP7 Modulates the Replication of Kaposi's Sarcoma-Associated Herpesvirus Latent Episomal DNA
... FIG 4 Deletion of aa 971 to 986 from the C-terminal domain of LANA abolishes or reduces USP7 binding. (A) LANA constructs harboring a GST tag were expressed in bacterial cells, bound to glutathione-Sepharose 4B ...
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Ubiquitin-specific protease 22 is critical to in vivo angiogenesis, growth and metastasis of non-small cell lung cancer
... About 4 weeks later, the cancer-bearing mice were euthanized, and both liver and lung metastasis were evaluated by counting visible tumor nodules per mouse and the total weight of excised lung and ...
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Ubiquitin specific protease 7 sustains DNA damage response and promotes cervical carcinogenesis
... a ubiquitin mutant with all lysine residues replaced by arginine except K48 (K48-only) or K63 (K63-only) to differentiate ubiquitin species opposed by USP7 on poly- ubiquitinated ...Figure 4, ...
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Prognostic and clinicopathological significance of ubiquitin-specific protease 22 overexpression in cancers: evidence from a meta-analysis
... As shown in Figure 4 and Table 2, increased USP22 was significantly associated with histological grade (RR = 0.72, 95% CI: 0.66–0.78, P = 0.002), advanced TNM stage (RR = 0.55, 95% CI: 0.49–0.61, P = 0.000), ...
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Ubiquitin-specific protease 14 regulates c-Jun N-terminal kinase signaling at the neuromuscular junction
... in 4-week-old mice, we found a greater than 100-fold increase in transcripts of the fetal AChR-γ subunit in TgUsp14CA gastrocnemius mus- cles compared to wild type muscles (Figure ...
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Prevalence of Plasmodium falciparum delayed clearance associated polymorphisms in adaptor protein complex 2 mu subunit (pfap2mu) and ubiquitin specific protease 1 (pfubp1) genes in Ghanaian isolates
... Results: In all, 11.1% (9/81) of the isolates carried the wildtype genotypes for both genes. A total of 164 pfap2mu mutations were detected in 67 isolates whilst 271 pfubp1 mutations were observed in 72 isolates. The ...
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Ubiquitin specific protease 19 involved in transcriptional repression of retinoic acid receptor by stabilizing CORO2A
... (Figure 4) when we hypothesized that the expression of USP19 also affects the transcription level of ...siRNA specific for USP19 resulted in a substantial increase in RARE luciferase activity (Figure 6C and ...
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Negative regulation of NF-κB signaling in T lymphocytes by the ubiquitin-specific protease USP34
... In addition to NF-κB, TCR ligation kindles various signal- ing pathways including Nuclear factor of activated T-cells (NFAT) and the Mitogen-activated protein kinase (MAPK) Extracellular signal-regulated kinases (ERK) ...
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Ubiquitin-specific protease 7 is a drug-able target that promotes hepatocellular carcinoma and chemoresistance
... The paraffin-embedded tissues sections from 100 matched pairs of liver adenocarcinoma and nonmalig- nant liver tissue of the same patients. The tissues were heated at 60 °C for 45 min and deparaffinized using a graded ...
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Reciprocal Activities between Herpes Simplex Virus Type 1 Regulatory Protein ICP0, a Ubiquitin E3 Ligase, and Ubiquitin-Specific Protease USP7
... USP7 degradation, HFFF-2 cells were infected with wild-type HSV-1 at multiplicities of infection of 1, 3, and 10 PFU per cell, and then samples were harvested at increasing times after infection and analyzed for USP7 ...
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Ubiquitin-specific protease 7 expression is a prognostic factor in epithelial ovarian cancer and correlates with lymph node metastasis
... the Matrigel invasion chambers and cultured for 12 hours. Then, cells were fixed with 4% paraformaldehyde for 30 minutes and stained with 0.05% gentian violet for 1 hour. Cells on the upper surface were carefully ...
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Autocatalytic Activity of the Ubiquitin-Specific Protease Domain of Herpes Simplex Virus 1 VP1-2
... its ubiquitin cleavage ac- tivity in vitro, and while the corresponding mutation in the context of a recombinant virus had a modest though detectable effect on replication (4-fold lower yields and up to 50% ...
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Original Article Ubiquitin-specific protease 22 as a prognostic biomarker in patients with various cancers: a meta-analysis
... exclusion criteria was as follows: (1) review arti- cles, letters, conference abstract, or laboratory articles; (2) non-human studies; (3) non-English articles; (4) overlapping studies; (5) lacking key information ...
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Mutagenesis of the Active-Site Cysteine in the Ubiquitin-Specific Protease Contained in Large Tegument Protein pUL36 of Pseudorabies Virus Impairs Viral Replication In Vitro and Neuroinvasion In Vivo
... After infection with PrV-UL36(C 26 S), a considerably pro- longed mean time to death of 111 h was observed. However, time to death of individual mice varied rather widely between 96 and 120 h p.i.. First clinical signs ...
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Ubiquitin-specific protease 3 promotes cell migration and invasion by interacting with and deubiquitinating SUZ12 in gastric cancer
... The ubiquitin-proteasome pathway is an important intracellular protein degradation regulatory system ...The ubiquitin-specific proteases (USPs) are the largest subclass of DUBs ...moving ...
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Assess the expression of ubiquitin specific protease USP2a for bladder cancer diagnosis
... RNA was isolated from tissue using 1 ml of TRIzol (Invitrogen, Carlsbad, CA) with homogenization in a 5- ml glass tube. The homogenate was transferred to a 1.5-ml tube and then mixed with 200 ml of chloroform. After ...
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