Universal 16S rRNA PCR
Contamination and Sensitivity Issues with a Real Time Universal 16S rRNA PCR
... the PCR mixture with exogenous DNA a ...a universal 16S rRNA PCR can be hindered by problems with contami- nation of reagents which may be derived from a bacterial source, such as Taq ...
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Use of Universal 16S rRNA Gene PCR as a Diagnostic Tool for Venous Access Port Related Bloodstream Infections
... the universal 16S rRNA gene using PCR has improved the diagnostic yield of microbiological ...of 16S rRNA PCR for the prediction of VAP-related bloodstream infection ...
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Diagnosis of Bacteremia in Whole Blood Samples by Use of a Commercial Universal 16S rRNA Gene Based PCR and Sequence Analysis
... DNA isolation. As advised by the manufacturer, DNA was extracted and isolated from 1-ml duplicates of whole-blood samples (in children, single 1-ml samples only were drawn) using the SepsiTest kit. The kit includes a ...
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Comparison of Different DNA Fingerprinting Techniques for Molecular Typing of Bartonella henselaeIsolates
... of 16S rRNA genes. Universal prokaryotic prim- ers TPU1 and RTU8 (Table 2) were used to amplify a 1,500-bp fragment of the 16S rRNA gene from the purified genomic DNA of the cultured ...
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SSCP Analysis of V3 Region of 16S rRNA for the Characterization of Rumen Microbiome of Goat
... (SSU) rRNA sequences can potentially provide a complete description of the rumen ...the PCR- SSCP analysis of V3 region of 16S rRNA as a potential target to characterize the rumen microbiome ...
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DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
... the 16S rRNA gene of bacteria which can be amplified by the universal PCR ...of universal primers is used for the amplification of the conserved region ofthe 16S rRNA ...
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Comparison of PCR Electrospray Ionization Mass Spectrometry with 16S rRNA PCR and Amplicon Sequencing for Detection of Bacteria in Excised Heart Valves
... universal rRNA gene PCR assay of extracted bacterial DNA was per- formed in a GeneAmp PCR system 9700 (Thermo Fisher Scientific, Wal- than, MA, ...UMD Universal kit provides paired ...
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Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae).
... The universal 16S rRNA primer set for polymerase chain reaction (PCR) amplification was as follows: 27f (5'-GAGTTTGATYMTGGCTCAG-3' (Ludwig et ...1986). PCR amplification was performed ...
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DETECTION OF SALMONELLA TYPHI AND HELICOBACTER GENUS IN GALLSTONE PATIENTS
... for universal 16SrRNA gene was subjected to PCR for amplifying bacterial Helicobactergenus16S rRNA gene which detect ...specimens. PCR products for the Helicobacter genus 16S rRNAbased ...
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Role of Universal 16S rRNA Gene PCR and Sequencing in Diagnosis of Prosthetic Joint Infection
... The etiological diagnosis of prosthetic joint infection (PJI) requires the isolation of microorganisms from periprosthetic sam- ples. Microbiological cultures often yield false-positive and false-negative results. ...
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The development of a 16S rRNA gene based PCR for the identification of Streptococcus pneumoniaeand comparison with four other species specific PCR assays
... Extensive data-mining using commercial software (Inte- grated Database Network System IDNS™, SmartGene, Zug, Switzerland), which allows rapid screening of vali- dated published sequences of species of interest against ...
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Comparison of 16S rRNA Gene PCR and BACTEC 9240 for Detection of Neonatal Bacteremia
... a PCR-based assay for ruling out bacterial sepsis in the uninfected term neonate could potentially save significant health care dollars and reduce the emotional impact for ...a PCR-based assay for detecting ...
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Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen
... Aim: Whole genome sequencing and functional an- notation of Bacillus nealsonii strain AAU1, an amylo- lytic anaerobic spore forming isolate from ruminal contents of buffalo. Methods and Results: Morpho- logically, the ...
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Detection of Balamuthia Mitochondrial 16S rRNA Gene DNA in Clinical Specimens by PCR
... PCR is a useful diagnostic tool for identifying suspected cases of balamuthiasis. The technique is sufficiently sensitive to detect mitochondrial DNA extracted from less than a single ameba (lane 7, Fig. 4). It ...
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Ehrlichia chaffeensis, a new species associated with human ehrlichiosis
... Using universal primers that are conserved among eubacterial 16S rRNA genes, we were able to amplify, clone, and sequence 16S rDNA from all five currently recognized species of ehrlichia[r] ...
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Study and exploitation of diverse soil environments for novel natural product discovery using metagenomic approaches
... is PCR screening for genes of interest, often performed in tandem with phenotypic and activity screening methods, where host expression system incompatibility occurs as evidenced by Gabor et ...a PCR ...
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Rapid Identification of Yersinia enterocolitica in Blood by the 5′ Nuclease PCR Assay
... Man PCR was performed by using 100 ng to 1 fg of chromo- somal DNA as a template and the primers and probes described ...the 16S rRNA gene per cell, this would translate to 1 template, or 10 ...
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Two Step Scheme for Rapid Identification and Differentiation of Legionella pneumophila and Non Legionella pneumophila Species
... The differentiation of L. pneumophila and non-L. pneumo- phila was achieved by the enzymatic digestion analysis of the 226-bp fragment of the 16S rRNA gene from the PCR assay. This method was based ...
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Metagenomic Approach for Identification of the Pathogens Associated with Diarrhea in Stool Specimens
... addition, 16S rRNA gene sequencing is capable of identifying ...the 16S FIG 2 Microbial community profile of the diarrhea samples revealed by ...
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Touchdown Enzyme Time Release PCR for Detection and Identification of Chlamydia trachomatis, C pneumoniae, and C psittaci Using the 16S and 16S 23S Spacer rRNA Genes
... the 16S rRNA and 16S-23S spacer rRNA genes (11, 15, 33) for ...the PCR products predicted with these primers and their corresponding Chlamydia species were 315 bp with primer set CTR ...
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