Universal primers
PCR primers Universal primers for PCR am-
... the universal primers 1 and 2-T7-pro- moter and in mixtures at various 52:RB ratios, with a constant concentration of 52 and a series of decreasing RB concentrations, as indicated in the ...
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Universal Primers Used for Detection of Bacterial Meningitis
... ribosomal RNA and enzymatic digestion to produce specific fragments for each PCR products. In current study, each PCR products for bacterial targets produces a significant fragment that causes the detection process to be ...
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DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
... the universal PCR ...of universal primers is used for the amplification of the conserved region ofthe 16S rRNA gene, in common bacterial ...the universal PCR method, these bacteria are ...
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Comparing the Accuracy Rate of Two Different Universal Primers in Enteric Pathogen Diagnosis From Blood
... pairs universal primers (28). The 16sr RNA universal primer designed by Lu et ...blood. Primers designed by Klauseger et ...both universal primers showed higher accuracy rate for ...
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Universal Primers for Detection and Sequencing of Hepatitis B Virus Genomes across Genotypes A to G
... inside. Primers 251f-1190r (set 1a) and 595f-1797r (set 1b), 2300f-215r (set 2a) and 2819f-617r (set 2b), 1584f-2331r (set 3), and 1877f-2835r (set 4) are ...
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Use of PCR with Universal Primers and Restriction Endonuclease Digestions for Detection and Identification of Common Bacterial Pathogens in Cerebrospinal Fluid
... PCR primers capable of amplifying all eubacteria based on the conservative nature of the 16S rRNA genes (3, ...such primers and found that this universal PCR can amplify all bacteria that we have ...
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Designing universal primers for the isolation of DNA sequences encoding Proanthocyanidins biosynthetic enzymes in Crataegus aronia
... the primers were BLAST searched against the nucleotide databases of the Genome Database for Rosaceae ...designed primers and the DNA sequences of the targeted PAs genes in the Rosa- ceae family was very ...
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Universal primers that amplify RNA from all three flavivirus subgroups
... degenerate primers is that the con- centration of some permutations in the mixture is so small, due to their great multiplicity, that amplification is effectively ...
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Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers
... of primers for nearly full length HBV genome amplification (3181 bp, 40 bp shorter than full length) and four sets of walking primers for fragment amplification were finally ...such primers makes it ...
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A Vavraia like microsporidium as the cause of deadly infection in threatened and endangered Eurycea salamanders in the United States
... using universal primers previously used to detect micro- sporidia in wild quail ...these primers with the target ...specific primers for more sensitive detection that confirmed microsporidian ...
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Development of RAPD, DAMD and ISSR markers for authentication of medicinal plant Cassia auriculata and its adulterant Cassia surattensis
... oligonucleotide primers are used to amplify multi locus DNA markers which have distributed within the genome ...as primers. These primers target multiple loci within the genome to amplify Inter ...
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Molecular Diversity of Bacterial Isolated from Cozy and Cold Spring Water in Mosul / Iraq
... Figure 6: Agarose gel electrophoresis of 16srRNA gene 1500 bp bands using universal primers 27F & 1492R Lane 1: DNA ladder 100bp, lane 2: Serratia nematodiphila , lane[r] ...
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Isolation and molecular identification of Mycoplasma gallisepticum from chicken flocks
... Primers used to confirm the purity of MG isolates were previously described by Lauerman el ...These universal primers were used for detection of nine avian mycoplasmas by amplification of the ...
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A simple PCR based strategy for estimating species specific contributions in chimeras and xenografts
... To analyze tissues from chimeras or xenografts quantitatively, we developed a strategy involving species-specific primers that amplify RPL19. Protein sequence of RPL19 from amino acid #35-75 is 100% conserved ...
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Unbiased Characterization of Anopheles Mosquito Blood Meals by Targeted High-Throughput Sequencing
... Here, we describe a novel approach using next-generation sequencing technology to analyze the blood meal composition of individual mosquitoes in an unbiased manner. We first amplify DNA extracted from a single female ...
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Ehrlichia chaffeensis, a new species associated with human ehrlichiosis
... Using universal primers that are conserved among eubacterial 16S rRNA genes, we were able to amplify, clone, and sequence 16S rDNA from all five currently recognized species of ehrlichia[r] ...
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Molecular microbial ecology of contaminated marine sediment near Casey Station, Antarctica
... In summary, five conclusions can be drawn from this work: i DGGE with universal primers has a limited usefulness in monitoring sediment microbial communities ii multivariate statistical [r] ...
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PCR pathotyping of native Bacillus thuringiensis from Mizoram, India
... Bacillus thuringiensis is a ubiquitous, gram-positive and spore-forming bacterium. During sporula- tion, it produces intracellular crystal (cry) proteins, which are toxic to insects. The genetic diversity of B. ...
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Application of PCR for the Detection of Universal Fungi in Infant Food Products Sold in Sri Lanka
... Abstract- Infant food products were collected from supermarkets in Sri Lanka and investigated for contamination by fungi. The objective of this study was to develop a novel DNA extraction procedure and a PCR assay for ...
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Multiplex PCR Using Internal Transcribed Spacer 1 and 2 Regions for Rapid Detection and Identification of Yeast Strains
... Multiplex PCR amplification followed by either agarose gel electrophoresis (PCR-AGE) or microchip electrophoresis (PCR-ME) was used to test a total of 120 fungal strains. The internal transcribed spacer 1 (ITS1) and ITS2 ...
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