β-defensin 2
Fecal human β-defensin-2 (hBD-2) levels and gut microbiota patterns in preterm neonates with different feeding patterns
... Human β-defensin 2 levels. This study showed that hBD-2 levels in the formula milk predominant group were significantly higher compared with the breast milk only ...Human β-defensins ...
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A study on β-defensin-2 and histatin-5 as a diagnostic marker of early childhood caries progression
... and β-defensin-2 and the number of cariogenic species of bacteria, the occurrence of specific bacterial profiles depending on the levels of the determined pro- teins was ...Table 2 Salivary ...
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Human β-defensin-2 production from S. cerevisiae using the repressible MET17 promoter
... Here we describe the use of the repressible MET17 pro- moter for the secretion of human β-defensin-2 in shake flask cultures (SFC) using a highly productive S. cerevi- siae strain. This yeast strain ...
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Isoleucine, an Essential Amino Acid, Induces the Expression of Human β Defensin 2 through the Activation of the G Protein Coupled Receptor ERK Pathway in the Intestinal Epithelia
... Human β-defensin (HBD) is one of the pivotal anti-microbial peptides that are expressed in the colonic ...inducible β-defensin HBD2 by an essential amino acid, isoleucine (Ile) in colonic ...
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Keratinocyte Expression of Human β Defensin 2 following Bacterial Infection: Role in Cutaneous Host Defense
... Models such as ours that utilize cultured keratinocytes strat- ified to only a few cell layers and not fully differentiated (5) have limitations when conclusions about the role of CAPs in vivo are drawn. The ...
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Human β defensin 2 plays a regulatory role in innate antiviral immunity and is capable of potentiating the induction of antigen specific immunity
... HBD 2 at the C terminus of the RBD (residues 291–725) of the S1 domain were performed as described previously with minor modifications ...HBD 2 gene at its 3′ terminus was amplified by polymerase chain ...
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Respiratory epithelial cells require Toll-like receptor 4 for induction of Human β-defensin 2 by Lipopolysaccharide
... seeded in 8 well chamber slides (Nunc). Slides were fixed in methanol (AnalaR) for 5 minutes and labelled with goat anti-HBD-2 antibody (a gift from Dr Paul McCray, University of Iowa, Iowa City, IA, USA) 1:100 ...
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Antimicrobial peptide β-defensin-1 expression is upregulated in Alzheimer’s brain
... Mammalian β-defensins are an evolutionarily conserved group of cationic, non-glycosylated antimicrobial and immunomodulatory peptides that are expressed in all mammals, including humans ...human ...
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Vitamin D nutritional status and vitamin D regulated antimicrobial peptides in serum and pleural fluid of patients with infectious and noninfectious pleural effusions
... Methods: Serum and pleural fluid samples from 152 patients with pleural effusion were collected, corresponding to 45 transudates and 107 exudates, 51 infectious effusions (14 complicated and 37 non-complicated), 44 ...
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Antimicrobial activity of innate immune molecules against Streptococcus pneumoniae, Moraxella catarrhalis and nontypeable Haemophilus influenzae
... to β defensin- 2 or ...µg/ml β defensin-2 for 30 minute, and 1 mg/ml of human milk lysozyme for 30 minute, ...of β defensin-2 or lysozyme, NTHi show blebbing ...
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Induction of beta defensin 2 by NTHi requires TLR2 mediated MyD88 and IRAK TRAF6 p38MAPK signaling pathway in human middle ear epithelial cells
... secreted β-defensin functions as an chemoattractant for CCR6 positive immature dendritic and memory T cells, facilitating the transition from innate immunity to adap- tive immunity by producing inflammatory ...
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Host Defense Peptides in Wound Healing
... receptor 2 and subsequent acti- vation of the NF-κB cascade induces human β-defensin 2 ...receptor 2 medi- ates induction of the antimicrobial pep- tide hBD-2 in response to ...
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Synergistic effect of interleukin 1 alpha on nontypeable Haemophilus influenzae induced up regulation of human beta defensin 2 in middle ear epithelial cells
... of β-defensin 2 promoter ...of β -defensin 2 (DEFB4) (AF_071216) was isolated by PCR amplification with the following primers: 5'-GAG GTA CCT CCA TCC TTT ACT GTG ATG ATG CC-3' ...
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Vaginal innate immune mediators are modulated by a water extract of Houttuynia cordata Thunb
... human β -defensin 2 (hBD2) and secretory leukocyte protease inhibitor (SLPI), as well as cytokines and chemokines that play vital roles in mucosal innate immunity of the female reproductive ...
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THE RELATIONSHIP BETWEEN INTESTINAL PROTECTIVE MARKERS AND SERUM ENDOTHELIN 1 CONCENTRATIONS IN LOW BIRTH WEIGHT INFANTS WITH HYPOXIC ISCHEMIC ENCEPHALOPATHY
... The results of previous investigations have shown that different inflammatory factors may contribute to the pathogenesis of intestinal barrier injury, and most studies have focused on the pathological roles of cytokines ...
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Canine β defensin 1 (CBD1) gene as a possible marker for Leishmania infantum infection in dogs
... For L. infantum detection, the primers LEISH-1 (5'-AAC TTT TCT GGT CCT CCG GGT AG-3'), LEISH-2 (5'- ACC CCC AGT TTC CCG CC-3') and the TaqMan® probe FAM-5'-AAA AAT GGG TGC AGA AAT-3'-non- fluorescent quencher-MGB ...
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A dual targeted ?? defensin and exome sequencing approach to identify, validate and functionally characterise genes associated with bull fertility
... known β-defensin genes plus 1000 bp upstream of the transcription start site (a total of 387 kb) were used for Roche Nimblegen SeqCap EZ Developer bait design, per standard ...
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Regulation of bovine beta defensin expression
... As β-defensins are highly pleiotropic peptides which may have both pro- inflammatory and anti-inflammatory properties depending on the concentration, regulation of the level of expression may be the difference ...
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Mastitis Increases Mammary mRNA Abundance of β-Defensin 5, Toll-Like-Receptor 2 (TLR2), and TLR4 but Not TLR9 in Cattle
... (80°C, 2 min) to enhance the sensitivity of the hybridizations, cold shocked on ice, and subse- quently maintained on a heating plate at ...formamide, 2⫻ SSC [1⫻ SSC is ...in 2⫻ ...
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Genetic variation in bulls divergent for fertility
... targeted β-defensin sequencing GATK best practice pipeline were filtered to remove variants of low quality and which fall outside certain parameters, to reduce the number of false ...
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