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β-Gal

MDM2 turnover and expression of ATRX determine the choice between quiescence and senescence in response to CDK4 inhibition

MDM2 turnover and expression of ATRX determine the choice between quiescence and senescence in response to CDK4 inhibition

... We then performed another test of p53- independence. PD0332991 had already been shown to induce senescence in a collection of glioma cell lines [5] and one of these, SNB19, expresses a transcriptionally inactive mutant ...

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Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro

Effects of Cigarette Smoke Extracts on the Growth and Senescence of Skin Fibroblasts In Vitro

... SA β-gal in some conditions, such as serum deprivation [31] and confluence [32], SA β-gal is still widely used as a biomarker for cellular senescence ...SA β-gal activity was ...

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Patched controls the Hedgehog gradient by endocytosis in a dynamin dependent manner, but this internalization does not play a major role in signal transduction

Patched controls the Hedgehog gradient by endocytosis in a dynamin dependent manner, but this internalization does not play a major role in signal transduction

... wild-type Hh protein. (A) Scheme of the predicted HhGFP fusion protein processing. HhGFP-F, full-length protein; HhGFP-U, the unprocessed protein without the signal peptide; HhC, the C-terminal region of the processed ...

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Proof of principle: quality control of therapeutic cell preparations using senescence associated DNA methylation changes

Proof of principle: quality control of therapeutic cell preparations using senescence associated DNA methylation changes

... for replicative senescence in vitro [9]. We observed that it is particularly detected in very late, senescent passages [10], although other authors indicated that it is also up-regulated earlier in culture [11,12]. ...

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Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

... Current evidence implicates intervertebral disc degeneration as a major cause of low back pain, although its pathogenesis is poorly understood. Numerous characteristic features of disc degeneration mimic those seen ...

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MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

MiR-23a-depressed autophagy is a participant in PUVA- and UVB-induced premature senescence

... After ultraviolet irradiation, the protein expression of AMBRA1 was significantly increased in the Ad- AMBRA1-transfected group compared with the Ad- CNT group (Figure 8a and Figure S8). Experiments consistently ...

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Is senescence-associated β-galactosidase a marker of neuronal senescence?

Is senescence-associated β-galactosidase a marker of neuronal senescence?

... One of the features of cellular senescence is the activity of senescence- associated- β-galactosidase (SA-β-gal). The main purpose of this study was to evaluate this marker of senescence in aging ...

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Intrahepatic cholangiocarcinoma can arise from Notch mediated conversion of hepatocytes

Intrahepatic cholangiocarcinoma can arise from Notch mediated conversion of hepatocytes

... into the nucleus and removes the loxP-flanked stop cassette from the R26R allele, leading to permanent heritable expression of the β-gal or YFP gene. (B) Representative images of liver from mice that drank ...

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Original Article Decreased Survival and Hepato-Renal Pathology in Mice with C-Terminally Truncated GP73 (GOLPH2)

Original Article Decreased Survival and Hepato-Renal Pathology in Mice with C-Terminally Truncated GP73 (GOLPH2)

... Figure 3 The GP73/β-geo fusion protein is predominantly expressed in epithelial cells. Cryosections were stained for β-gal expression (light blue, arrowheads), and counterstained with Eosin Y and ...

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A chromatin insulator mediates transgene homing and very long range enhancer promoter communication

A chromatin insulator mediates transgene homing and very long range enhancer promoter communication

... Fig. 4. The eve-TER94 insulator is required for, and the eve promoter contributes to, long-range E-P communication. Staged embryos were subjected to anti-β-gal staining, except Q and R, which show anti-Eve ...

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Enzyme adsorption-induced activity changes: a quantitative study on TiO2 model agglomerates

Enzyme adsorption-induced activity changes: a quantitative study on TiO2 model agglomerates

... have proven to be an appropriate model system for the study of hydration-induced microstructural and elec- tronic property changes [16–20]. Only recently, we have employed such particle powders as model systems for ...

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Lentiviral-Mediated Transgene Expression Can Potentiate Intestinal Mesenchymal-Epithelial Signaling

Lentiviral-Mediated Transgene Expression Can Potentiate Intestinal Mesenchymal-Epithelial Signaling

... Immunohistochemical Analyses Liver, spleen, lung, and the intestine were processed for frozen sectioning and antibody staining as previ- ously described (21). GFP-expressing cells were identified in 5-μm- thick sections ...

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Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

Accelerated cellular senescence in degenerate intervertebral discs: a possible role in the pathogenesis of intervertebral disc degeneration

... Current evidence implicates intervertebral disc degeneration as a major cause of low back pain, although its pathogenesis is poorly understood. Numerous characteristic features of disc degeneration mimic those seen ...

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Retroviral transfer of the p16INK4a cDNA inhibits C6 glioma formation in Wistar rats

Retroviral transfer of the p16INK4a cDNA inhibits C6 glioma formation in Wistar rats

... We have observed a morphological alteration of the C6 cells upon infection with the pCLp16 virus. The control cells surviving G418 selection in the colony formation as- says (as in Figure 1) were not altered ...

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Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency

Neuronal Subtype Determines Herpes Simplex Virus 1 Latency-Associated-Transcript Promoter Activity during Latency

... LAT Promoter Activity in Neuronal Subtypes Journal of Virology A β-Gal β3-Tub 20μm NefH % of neurons β-Gal+ B Downloaded from http://jvi.asm.org/ on November 6, 2019 by guest CGRP-GFP Me[r] ...

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Utilizing the adjuvant properties of CD1d dependent NK T cells in T cell–mediated immunotherapy

Utilizing the adjuvant properties of CD1d dependent NK T cells in T cell–mediated immunotherapy

... than β-gal protein, with responses to the latter being detected only with α-GalCer as an adjuvant, followed by boosting with vaccinia encoding β-gal (Figure ...

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The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D Galactose induced aged rat model

The positive effects of Ginsenoside Rg1 upon the hematopoietic microenvironment in a D Galactose induced aged rat model

... senescence-associated β -galactosidase (SA- β -gal) staining, flow cytometric cell cycle phase distribution assay, CCK-8 cell proliferation assay, oxidative stress (reactive oxygen species [ROS], ...

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Restoration of tubular epithelial 
cells during repair of the postischemic 
kidney occurs independently of bone 
marrow derived stem cells

Restoration of tubular epithelial cells during repair of the postischemic kidney occurs independently of bone marrow derived stem cells

... bacterial β-gal or harboring the male Y chromosome exclusively in bone marrow-derived ...In β-gal chimeras, some tubular cells also appeared to express β-gal as assessed by ...

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Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis

Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis

... by β-gal staining and tdTomato ...of β-gal+/tdTomato + cells were ob- served in the cranial mesenchyme and the forming first pharyngeal arch (trigeminal neural crest-tg) of NKO mu- tants ...

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Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche

Induction of bone marrow-derived cells myogenic identity by their interactions with the satellite cell niche

... of β-galactosidase. Con- trol CXCR4 + BMCs(β-gal + ) participated in the formation of new myofibers with very low ...expressed β-galactosidase ...expressed β-galactosidase, and at day ...

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