vacuolar ATPase
The Vacuolar ATPase of Neurospora crassa Is Indispensable: Inactivation of the vma-1 Gene by Repeat-Induced Point Mutation
... Progeny from strains with an inactive endogenous - vma-1 gene were inviable unless a functional copy of the gene cosegregated, indicating that - the vacuolar ATPase is ess[r] ...
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Vacuolar ATPase of Neurospora crassa: electron microscopy, gene characterization and gene inactivation/mutation
... 2 Genes encoding three of the major subunits of the vacuolar ATPase and the homologous subunits of the mitochondrial FATPase have been isolated.. The exon/intron structures of the genes [r] ...
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Antiviral efficacy of nanoparticulate vacuolar ATPase inhibitors against influenza virus infection
... Notes: (A) Free diphyllin or diphyllin NP was added to MH-S cells and incubated for 72 hours. An alamarBlue assay was performed and cell viability was normalized to the value of untreated medium controls (100%). (B) Free ...
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Mutations in the yeast vacuolar ATPase result in the mislocalization of vacuolar proteins
... Vacuolar membrane proteins are less sensitive than soluble vacuolar proteins to changes in compartment acidification All three of the vat mutations also cause an accumulation of the prec[r] ...
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Role of the Vacuolar-ATPase in Sindbis Virus Infection.
... Following infection, the cells were incubated with BAF at 37˚C for (x) min post- infection, after which BAF was removed by washing the cells once. The percent of cells expr[r] ...
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The vacuolar ATPase is required for physiological as well as pathological activation of the Notch receptor
... furrow in mosaic discs (C). Mutant tissue is outlined in B⬘,C⬘. The regions of the tissue that accumulate Notch the most appear to express m-lacZ the least (compare B with B⬘). In C, m-lacZ is expressed at high level ...
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Role of the Vacuolar-ATPase in Sindbis Virus Infection
... (10, 11, 36, 37). The V-ATPase is responsible for maintaining pH homeostasis of the cell and for endosome acidification during endocytosis. Bafilomycin A 1 (BAF) is a commonly used inhibitor of the ...
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Molecular genetics of the yeast vacuolar H(+) ATPase
... Nine VMA genes that are indispensable for expression of vacuolar ATPase activity have been identified in the yeast Saccharomyces cerevisiae.. VMAJ, VMA2, VMA3, VMA5 and VMA6 were cloned [r] ...
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Genes Expressed in the Ring Gland, the Major Endocrine Organ of Drosophila melanogaster
... of vacuolar ATPase (V-ATPase) in the medial cells of the ring gland, which produce the juvenile hormone that controls progression through developmental ...
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Studies of Ebola Virus Glycoprotein-Mediated Entry and Fusion by Using Pseudotyped Human Immunodeficiency Virus Type 1 Virions: Involvement of Cytoskeletal Proteins and Enhancement by Tumor Necrosis Factor Alpha
... To test whether acidification is required for detection of entry and fusion with Ebola virus GP-pseudotyped virions, target cells were pretreated with the vacuolar ATPase inhibitor bafil[r] ...
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Characterization of Synthetic-Lethal Mutants Reveals a Role for the Saccharomyces cerevisiae Guanine-Nucleotide Exchange Factor Cdc24p in Vacuole Function and Na+ Tolerance
... The isolation of two different mutants, one affecting vacuole morphology and the other in a vacuolar ATPase gene, that both exhibit synthetic-le- thality with cdc24-4h [r] ...
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Requirement for vacuolar proton-ATPase activity during entry of influenza virus into cells.
... Apart from the contribution that low pH makes to modifi- cation of viral glycoprotein conformation, it is conceivable that the pH gradient created by the v-[H 1 ]ATPase pumps is also required for virus entry, as ...
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Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+ ATPase Recycling
... V- ATPase, which utilizes unique clathrin- and caveolin-inde- pendent mechanisms (51, 64), has not been demonstrated ...V- ATPase-containing microvilli ...
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Regulation of sodium dependent phosphate transport in osteoclasts
... Osteoclasts are the primary cells responsible for bone re- sorption. They are exposed to high ambient concentrations of inorganic phosphate (Pi) during the process of bone re- sorption and they possess specific ...
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Dysregulation of autophagy in chronic lymphocytic leukemia with the small molecule Sirtuin inhibitor Tenovin 6
... To distinguish between these two possibilities, lysosomal degradation was blocked by treating cultures with 10 nm bafilomycin-A1 that targets vacuolar-type H1-ATPase, such that stimulati[r] ...
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The Vacuolar Ca2+ ATPase Pump Pmc1p Is Required for Candida albicans Pathogenesis
... While most Pmc1p homologs are not essential for fungal viability in vitro, their function appears to be essential for the survival of C. albicans, C. neoformans and A. fumigatus in vivo, i.e., within mammalian tissue ...
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Preconditioning rabbit cardiomyocytes: role of pH, vacuolar proton ATPase, and apoptosis
... the vacuolar proton ATPase (VPATPase) prevented the effect of preconditioning to reduce metabolic inhibition-induced acidosis, loss of viability, and DNA ...
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RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA
... a vacuolar-type H + -ATPase (V-ATPase) transcript, was sufficient for inducing a lethal response that differed between closely related species within the genus ...
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A V0 ATPase dependent apical trafficking pathway maintains the polarity of the intestinal absorptive membrane
... Fig. 6. V0-ATPase silencing induces microvillus atrophy in C. elegans. (A) Visualization of microvilli by super- resolution (upper panels, 96 h RNAi, ERM-1::GFP) and TEM (lower panels, 72 h RNAi) analysis. Control ...
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Regulation of Vacuolar H+-ATPase Activity by the Cdc42 Effector Ste20 in Saccharomyces cerevisiae
... sustaining vacuolar pH gra- dients during nutrient mobilization by vacuolar antiporters and allowing more efficient mobilization of intracellular reserves re- quired for cell ...and vacuolar ...
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