Vacuolar h
Biogenesis of the yeast vacuolar H(+) ATPase
... The assembly of the yeast vacuolar H + -ATPase requires the attachment of several cytoplasmically oriented, peripheral subunits the Vi sector to a complex of integral membrane subunits t[r] ...
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vph6 mutants of Saccharomyces cerevisiae require calcineurin for growth and are defective in vacuolar H(+)-ATPase assembly.
... V-ATPase subunits (Figure 3, lanes 5 and 6). These results suggest that the VPH6 protein plays a role in vacuolar H'-ATPase assembly. Yeast strains with defects in va[r] ...
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Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+ ATPase Recycling
... The role of the actin cytoskeleton in regulating mem- brane protein trafficking is complex and depends on the cell type and protein being examined. Using the epidid- ymis as a model system in which luminal acidification ...
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Structure of the vacuolar H+-ATPase rotary motor reveals new mechanistic insights
... Vacuolar H + -ATPases are multisubunit complexes that operate with rotary mechanics and are essential for membrane proton transport throughout eukary- ...
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Vacuolar H+-ATPase Works in Parallel with the HOG Pathway To Adapt Saccharomyces cerevisiae Cells to Osmotic Stress
... isolated vacuolar vesicles from wild-type cells exposed to high salt conditions (500 mM NaCl) for 30 min just before lysis and compared vacuolar vesicles purified from salt-shocked cells to those from cells ...
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Circadian rhythm of acidification in insect vas deferens regulated by rhythmic expression of vacuolar H+ ATPase
... Recent studies have demonstrated that the peripheral tissues of vertebrates and invertebrates contain circadian clocks; however, little is known about their functions and the rhythmic outputs that they generate. To ...
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Regulation of Vacuolar H+-ATPase Activity by the Cdc42 Effector Ste20 in Saccharomyces cerevisiae
... Vacuolar H ⫹ -ATPases (V-ATPases) are ATP-driven proton pumps that acidify organelles, such as the lysosome, the Golgi apparatus, and endosomes, in all eukaryotic cells (12, ...the vacuolar subunit ...
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Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of plasma membrane H+-ATPase, vacuolar H+-PPase, alkaline and acid phosphatases
... Gomes, E., Gordon-Weeks, R., Simon-Plas, F., Pugin, A., Milat, M-L., Leigh, R. A. and Blein, J-P. 1996. Cercospora beticola toxins. Part XVII. The role of beticolin/Mg2+ complexes in their biological activity. Study of ...
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Biochemical and functional characterization of the actin binding activity of the B subunit of yeast vacuolar H+ ATPase
... Vacuolar H + -ATPases (V-ATPases) are multi-subunit enzymes that play vital housekeeping roles in eukaryotic cells (Kane, 2006; Inoue et al., 2003; Harvey, 1992; Nelson and Harvey, 1999). These roles ...
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Assembly and regulation of the yeast vacuolar H(+) ATPase
... One final factor that appears to be critical for regulation of V-ATPase assembly state in response to glucose is the activity of the V-ATPase itself. Disassembly of the yeast V-ATPase in response to glucose deprivation ...
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Requirement for Vacuolar H+-ATPase Activity and Ca2+ Gradient during Entry of Rotavirus into MA104 Cells
... virus alone, inhibition of protein synthesis by ␣-sarcin reached its maximum (80%) after 60 min of addition of the label. Therefore, there is a lag between the removal of ␣-sarcin from the extracellular medium and the ...
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Cloning and molecular characterisation of the trout (Oncorhynchus mykiss) vacuolar H(+) ATPase B subunit
... 12 h:12 h light:dark photoperiod and were fed ad libitum on alternate days using a commercial pelleted fish diet but were not fed for 48 h prior to ...
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Prognostic significance and function of the vacuolar H -ATPase subunit V1E1 in esophageal squamous cell carcinoma
... V-ATPase is a ubiquitously expressed ATP- dependent proton pump, and plays a role in maintaining a relatively neutral intracellular pH, an acidic luminal pH, and an acidic extracellular pH [12]. V-ATPase-dependent ...
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Saccharomyces cerevisiae Vacuolar H+-ATPase Regulation by Disassembly and Reassembly: One Structure and Multiple Signals
... yeast vacuolar membrane, but func- tional interactions of phosphoglycerate kinase or pyruvate kinase (glycolytic enzymes that produce ATP) with V-ATPase have yet to be ...
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The osteoclast proton pump differs in its pharmacology and catalytic subunits from other vacuolar H(+) ATPases
... Analysis of proton transport by inside-out vesicles derived from highly purified chicken osteoclast membranes has revealed the presence of a novel type of multisubunit vacuolar-like H + [r] ...
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Molecular genetics of the yeast vacuolar H(+) ATPase
... Nine VMA genes that are indispensable for expression of vacuolar ATPase activity have been identified in the yeast Saccharomyces cerevisiae.. VMAJ, VMA2, VMA3, VMA5 and VMA6 were cloned [r] ...
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Composition and assembly of the yeast vacuolar H(+) ATPase complex
... (subunit a) is a polytopic membrane protein encoded by two genes in yeast, designated VPH1 and STV1 (Manolson et al., 1992, 1994). The Vph1p is a subunit of the V-ATPase complex found on the vacuolar membrane, and ...
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Flexibility within the Rotor and Stators of the Vacuolar H + -ATPase
... crystal structures give only a snapshot of a static, low energy state and are unable to show to what level the stator can resist flexing. However, crystal lattice constraints are not a factor in EM studies of single ...
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The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
... SW1088, T98G or LN229 glioma cell lines were purchased from ATCC (Manassas, VA, USA) and cultured as suggested by the manufacturer. Protein extract from E18 primary rat hippocampal neurons (E18 RHN) were a generous gift ...
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The vacuolar H(+) ATPase one of the most fundamental ion pumps in nature
... Recently, we observed that removal of the catalytic sector of eukaryotic V-ATPases from the membrane failed to generate increased proton conduction across the depleted membranes Beltran [r] ...
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