Vacuolar H+-ATPase
vph6 mutants of Saccharomyces cerevisiae require calcineurin for growth and are defective in vacuolar H(+)-ATPase assembly.
... V-ATPase subunits (Figure 3, lanes 5 and 6). These results suggest that the VPH6 protein plays a role in vacuolar H'-ATPase assembly. Yeast strains with defects in va[r] ...
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Biogenesis of the yeast vacuolar H(+) ATPase
... The assembly of the yeast vacuolar H + -ATPase requires the attachment of several cytoplasmically oriented, peripheral subunits the Vi sector to a complex of integral membrane subunits t[r] ...
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Assembly and regulation of the yeast vacuolar H(+) ATPase
... Most of the experiments described in the previous sections are directed towards understanding what happens when the assembly state of the V-ATPase changes, but one of the most fundamental questions is how the cell ...
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Biochemical and functional characterization of the actin binding activity of the B subunit of yeast vacuolar H+ ATPase
... actin-binding domain of the B subunit was altered by mutations that reduced or eliminated the actin-binding activity. Mutants assembled properly with endogenous yeast subunits when expressed in B subunit-null yeast and ...
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Requirement for Vacuolar H+-ATPase Activity and Ca2+ Gradient during Entry of Rotavirus into MA104 Cells
... The acidification component is required for the entry of numerous enveloped and nonenveloped viruses (17, 20, 24, 26). A low pH is required to elicit a fusion-inducing or perme- abilizing conformational change of one or ...
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Regulation of Vacuolar H+-ATPase Activity by the Cdc42 Effector Ste20 in Saccharomyces cerevisiae
... V-ATPase activity and filamentous growth. Since STE20 and VMA13 interact genetically and at the protein level, we examined whether the V-ATPase contributes to processes that are regulated by Ste20. vma13 ⌬ ...
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Circadian rhythm of acidification in insect vas deferens regulated by rhythmic expression of vacuolar H+ ATPase
... the unicellular organism Gonyaulax (Roenneberg and Merrow, 1999) and in the goldfish and rabbit retinas (Dmitriev and Mangel, 2000, 2001). However, the molecular and cellular bases of these rhythms have not been ...
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Structure of the vacuolar H+-ATPase rotary motor reveals new mechanistic insights
... M. sexta V-ATPase was extracted and purified and its activity tested as pre- viously described (Huss et al., 2002). Sample quality was checked by nega- tive stain microscopy, revealing a monodisperse sample. ...
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A novel subunit of vacuolar H(+) ATPase related to the b subunit of F ATPases
... mutant with a phenotype identical to those of all the other null mutations in V-ATPase subunits (Nelson and Nelson, 1990; Umemoto et al. 1990; Noumi et al. 1991). This phenotype could be complemented by ...
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Saccharomyces cerevisiae Vacuolar H+-ATPase Regulation by Disassembly and Reassembly: One Structure and Multiple Signals
... sembly, which reversibly inactivates them. Reversible disassembly is intertwined with glycolysis, the RAS/cyclic AMP (cAMP)/ protein kinase A (PKA) pathway, and phosphoinositides, but the mechanisms involved are elusive. ...
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Immunolocalization of the 17 kDa vacuolar H(+) ATPase subunit c in Heliothis virescens midgut and malpighian tubules with an anti peptide antibody
... In the intermolt midgut, the anti-17 kDa protein affinity- purified antibody did not consistently label areas of cell–cell contact. If the 17 kDa protein is a constituent of gap junctions in the midgut, it is possible ...
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Vacuolar H+-ATPase Works in Parallel with the HOG Pathway To Adapt Saccharomyces cerevisiae Cells to Osmotic Stress
... CHI interactions between the V-ATPase and members of the HOG pathway. We exploited the phenotype of the VMA13/ vma13⌬ hemizygote to screen for other genes that have CHI in- teractions with vma13 ⌬ . To do this, we ...
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Prognostic significance and function of the vacuolar H -ATPase subunit V1E1 in esophageal squamous cell carcinoma
... We found that V-ATPase V1E1 depletion induced a metabolic switch that led to decreased phosphorylation of PKM2 Tyr 105. PKM2 is a key molecule that induces glucose uptake and expression of glycolytic enzymes [30]. ...
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The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
... For immunofluorescence experiments, glioma monolayer cultures were grown on cover-glasses whereas neurospheres were cyto-spinned on charged slides (Thermo Scientific, Waltham, MA, USA). Glioma cultures were stained with ...
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Cloning and molecular characterisation of the trout (Oncorhynchus mykiss) vacuolar H(+) ATPase B subunit
... 12 h:12 h light:dark photoperiod and were fed ad libitum on alternate days using a commercial pelleted fish diet but were not fed for 48 h prior to ...
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Flexibility within the Rotor and Stators of the Vacuolar H + -ATPase
... rotary ATPase, in particular for the membrane bound subunit-a, prevents the application of standard atomistic modelling and molecular simulation ...V- ATPase show two low frequency modes (Figure 5) – ...
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Composition and assembly of the yeast vacuolar H(+) ATPase complex
... our efforts has been towards identifying which of the V- ATPase subunits interact with the assembly factors. Additional crosslinking analysis revealed a direct interaction between newly synthesized 100 kDa subunit ...
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Molecular genetics of the yeast vacuolar H(+) ATPase
... Nine VMA genes that are indispensable for expression of vacuolar ATPase activity have been identified in the yeast Saccharomyces cerevisiae.. VMAJ, VMA2, VMA3, VMA5 and VMA6 were cloned [r] ...
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Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+ ATPase Recycling
... V- ATPase-containing vesicles partially co-localized with HRP- containing endosomes (yellow), indicating that a significant amount of V-ATPase was located in the endocytic compart- ...V- ATPase, were ...
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The vacuolar H(+) ATPase one of the most fundamental ion pumps in nature
... Recently, we observed that removal of the catalytic sector of eukaryotic V-ATPases from the membrane failed to generate increased proton conduction across the depleted membranes Beltran [r] ...
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