vacuolar type H+-ATPase
A vacuolar type H+ ATPase and a Na+/H+ exchanger contribute to intracellular pH regulation in cockroach salivary ducts
... enhanced H + transport across the apical membrane into the gland lumen (Dames et ...V- ATPase binds directly to the glycolytic enzyme aldolase and that its activity is stimulated by ...
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Novel and potential physiological roles of vacuolar type H+ ATPase in marine organisms
... cell type, the lack of cell cultures and their compromised viability upon exposure to stressful conditions (Breton and Brown, 1998; Gong et ...mediates H + reabsorption and energizes apical ...
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Characterization of Acaricide Resistance, Plant-Mediated RNAi Against Two-Spotted Spider Mites (Tetranychus urticae Koch), and Assessing Off- and Non-Target Effects
... querying Vacuolar-type H + -ATPase subunit A transgene sequence against the available genome database for cabbage ...wild type, the potential non-target RNAi silencing of spider mite ...
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RNAi Targeting of Manduca sexta Using Chloroplast-Encoded Long dsRNA
... a vacuolar-type H + -ATPase (V-ATPase) transcript, was sufficient for inducing a lethal response that differed between closely related species within the genus ...
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The vesicle-associated function of NOD2 as a link between Crohn’s disease and mycobacterial infection
... the ATPase do- main of the proton-pumping F1-ATPase, which in turn is highly similar to that of Vacuolar-type H + -ATPase (V-ATPase) ...
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Composition and assembly of the yeast vacuolar H(+) ATPase complex
... (subunit a) is a polytopic membrane protein encoded by two genes in yeast, designated VPH1 and STV1 (Manolson et al., 1992, 1994). The Vph1p is a subunit of the V-ATPase complex found on the vacuolar ...
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Vacuolar type proton pumps in insect epithelia
... proton pump are largely unknown as the first examples of phosphorylation of some subunits are now being identified. But phosphorylation is just one of many mechanisms for post- translational modification of proteins. ...
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The Midgut as a Model System for Insect K+ Transporting Epithelia: Immunocytochemical Localization of a Vacuolar Type H+ Pump
... A Purified vacuolar-type ATPase about 3.5 fig protein per lane from goblet cell apical membrane, amido black staining of total protein; B-D immunostaining of vacuolar-type ATPase by prot[r] ...
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Changes in H+ translocating vacuolar type ATPase in the anterior silk gland cell of Bombyx mori during metamorphosis
... Anterior silk glands from feeding larvae and spinning larvae were collected and fixed in Bouin’s solution (water-saturated picric acid:formalin:acetic acid, 15:5:1 by volume) for 5–6 h at room temperature (20–25 ...
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Modulation of the Actin Cytoskeleton via Gelsolin Regulates Vacuolar H+ ATPase Recycling
... The actin cytoskeleton is a dynamic structure that is in- volved in a variety of cellular processes, including cell motility (1– 6), division (7), adhesion (8), and vesicle trafficking (9 –20). In eukaryotic cells, a ...
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Distribution and serotonin induced activation of vacuolar type H+ ATPase in the salivary glands of the blowfly Calliphora vicina
... V- ATPase on the apical domain and that changes in V-ATPase activity and assembly during our experiments can be largely attributed to pump molecules within the apical domain of the secretory ...Third, ...
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A novel subunit of vacuolar H(+) ATPase related to the b subunit of F ATPases
... The F- and V-ATPases are protein complexes related both in their subunit structure and in their evolutionary origin (Nelson, 1992a,b,c). Moreover, it is assumed that their mechanism of action is similar. Both enzymes ...
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Flexibility within the Rotor and Stators of the Vacuolar H + -ATPase
... The 3D cryoEM map of intact Manduca V-type ATPase [12] was used to obtain a coarse-grained (CG) representation of the ATPase complex. Using a topology-preserving algorithm [49] as implemented in the ...
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Assembly and regulation of the yeast vacuolar H(+) ATPase
... Changes in carbon source generate a wide variety of transcriptional and post-translational changes in yeast cells generally directed towards efficient utilization of the available nutrients (for reviews, see Johnston and ...
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Regulation of Vacuolar H+-ATPase Activity by the Cdc42 Effector Ste20 in Saccharomyces cerevisiae
... indicated plasmid combinations were spotted either onto medium lacking histidine and leucine to select for the plasmids or onto medium lacking histidine and leucine and supplemented with 5-FOA to monitor protein ...
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The vacuolar H+ ATPase is a novel therapeutic target for glioblastoma
... We next evaluated whether V-ATPase G1 was significantly enriched in GBM and if was correlated to GBM patients’ clinical features or prognosis. Therefore we analyzed V-ATPase G1 immunoreactivity in a larger ...
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Structure of the vacuolar H+-ATPase rotary motor reveals new mechanistic insights
... M. sexta V-ATPase was extracted and purified and its activity tested as pre- viously described (Huss et al., 2002). Sample quality was checked by nega- tive stain microscopy, revealing a monodisperse sample. ...
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Effects of efrapeptin and destruxin, metabolites of entomogenous fungi, on the hydrolytic activity of vacuolar type ATPase identified on the brush border membrane vesicles of Galleria mellonella midgut and on plant membrane bound hydrolytic enzymes
... of ATPase activity ^ the destruxins inhibit V-type ATPases from Saccha- romyces cerevisiae [22] and the efrapeptins inhibit photophosphorylation in plants and bacteria [23] and oxidative phosphorylation in ...
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Biogenesis of the yeast vacuolar H(+) ATPase
... The assembly of the yeast vacuolar H + -ATPase requires the attachment of several cytoplasmically oriented, peripheral subunits the Vi sector to a complex of integral membrane subunits t[r] ...
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Regulation of sodium dependent phosphate transport in osteoclasts
... homogenized using a Dounce homogenizer, and then the homoge- nate was centrifuged at 10,000 rpm for 10 min in a rotor (Sorvall SS- 34; DuPont Co., Wilmington, DE). The supernatant was collected and ultracentrifuged at ...
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