viral cryo electron microscopy
Cryo-Electron Microscopy Structure of Porcine Deltacoronavirus Spike Protein in the Prefusion State
... Structures, functions, and evolution of S1 subdomains. The structures of SD1 and SD2 are similar to those of their counterparts in ␣ - and  -coronavirus spikes (Fig. 3B). SD1 adopts a small  -sandwich fold containing ...
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A 3.0-Angstrom Resolution Cryo-Electron Microscopy Structure and Antigenic Sites of Coxsackievirus A6-Like Particles
... lethal viral challenges (19, 20), indicating that CVA6 VLPs represent an excellent CVA6 vaccine ...by cryo-electron microscopy (cryo-EM) single-particle ...
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Cryo-electron Microscopy Structures of Novel Viruses from Mud Crab Scylla paramamosain with Multiple Infections
... ABSTRACT Viruses associated with sleeping disease (SD) in crabs cause great eco- nomic losses to aquaculture, and no effective measures are available for their pre- vention. In this study, to help develop novel antiviral ...
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Recent advances in retroviruses via cryo electron microscopy
... the viral protease, the polyprotein Gag is cleaved in multiple positions, and these induce a dra- matic rearrangement of the virion core, leading to the formation of an infectious particle ...denying viral ...
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Cryo-electron Microscopy Study of the Genome Release of the Dicistrovirus Israeli Acute Bee Paralysis Virus
... Viruses of the family Dicistroviridae have nonenveloped icosahedral capsids protect- ing linear, single-stranded, positive-sense RNA genomes of 8,500 to 10,200 nucleotides (20). The genomes of dicistroviruses include two ...
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Novel inter subunit contacts in Barley Stripe Mosaic Virus revealed by Cryo Electron Microscopy
... To understand if the multiple RNAs of the wtBSMV could ac- count for existence of two types of virions, we analyzed images of chimeric BSMV (chBSMV), in which the single ssRNA of TMV has been coated with non-modified ...
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The structures of a naturally empty cowpea mosaic virus particle and its genome-containing counterpart by cryo-electron microscopy
... any viral particles in extracts prepared from ...by electron microscopy ...the viral yield from infiltrated leaves was markedly reduced, no systemic infection was observed and RNA was not ...
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Cryo-electron Microscopy Structures of Expanded Poliovirus with VHHs Sample the Conformational Repertoire of the Expanded State
... The GH loop of VP3 and its relevance to receptor-triggered poliovirus expan- sion. The GH loop of VP3 (residues 169 to 188) (Fig. 6) lies in the center of the icosahedral 5-3-3 triangle and directly to the east of the ...
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Catching a Virus in the Act of RNA Release: a Novel Poliovirus Uncoating Intermediate Characterized by Cryo-Electron Microscopy
... myrVP4 and the amino-terminal extensions of the major VPs line the interior surface of the viral protein shell, thus forming a network that governs assembly and stabilizes the mature 160S poliovirion (Fig. 1C). A ...
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Characterization of Influenza Vaccine Hemagglutinin Complexes by Cryo-Electron Microscopy and Image Analyses Reveals Structural Polymorphisms
... the viral surface suggested the matching of 4-nm transmembrane regions ...of viral HA and HA com- plex sizes suggests that the transmembrane regions of constituent HA molecules are in close proximity and ...
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Cryo-Electron Microscopy Structure of Seneca Valley Virus Procapsid
... Our structure shows a large dodecahedral cage on the interior of the capsid, with densities sized to accommodate RNA helices (Fig. 9). The cage is not evident at 3.8 Å and becomes clear only at resolutions lower than ⬃ 6 ...
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Cryo electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate
... surface-expressed viral glycoprotein hemagglutinin (HA) and are generally exclusively effective against infectious viral populations that match the vaccine strain ...
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The U4 Antibody Epitope on Human Papillomavirus 16 Identified by Cryo-electron Microscopy
... the viral capsid proteins have been applied successfully to protect against high-risk HPV, the efficacy of vaccines is genotype specific, and vaccines provide little therapeutic benefit against existing infections ...
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Cryo-Electron Microscopy Structure of Lactococcal Siphophage 1358 Virion
... that viral infection is neutralized when this site is blocked by a camelid nanobody (10, ...complete electron microscopy (EM) structure of phage p2 (19) and phage TP901-1 (3) were newly ...
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Structure of Flexible Filamentous Plant Viruses
... the viral crop damage in the world (37), infecting most economi- cally important crops ...either viral symmetry or coat protein folding. Indeed, reports of viral symmetry until now appeared to ...
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Correlative cryo super-resolution light and electron microscopy on mammalian cells using fluorescent proteins
... Cryo super-resolution correlative light and electron microscopy on lipid nanotubes. We synthe- sised lipid nanotubes with 1 mol % Ni-NTA-conjugated lipids and labelled these with His-tagged ...
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Topographic contrast of ultrathin cryo-sections for correlative super-resolution light and electron microscopy
... Fluorescence microscopy reveals molecular expression at nanometer resolution but lacks ultrastructural context ...results. Electron microscopy provides this contextual subcellular detail, but protein ...
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Structure, assembly and dynamics of macromolecular complexes by single particle cryo-electron microscopy
... To gain insights into the function of TFIID, the inter- action of yeast TFIID with the promoter DNA was stu- died in the presence of TFIIA (a general transcription factor required for specific recognition of the TATA ...
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The cryo electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation
... How the stressosome, the epicenter of the stress response in bacteria, transmits stress signals from the environment has remained elusive. The stressosome consists of multiple copies of three proteins RsbR, RsbS and ...
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Detection of truncated virus particles in a persistent RNA virus infection in vivo.
... of viral protein detected by immunohistochemistry, in viral RNA by PCR amplification, and in IHNV-truncated particles by immunogold electron microscopy confirmed the presence of IHNV in the ...
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