Virus Induced Gene Silencing
A Cytological Analysis of Wheat Meiosis Targeted by Virus-Induced Gene Silencing (VIGS)
... orthologous gene function in wheat is to utilize virus-induced gene silencing (VIGS) ...determining gene function in wheat, so further analyses using TILLING or CRISPR/Cas may be ...
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Solanum venturii, a suitable model system for virus-induced gene silencing studies in potato reveals StMKK6 as an important player in plant immunity
... Background: Virus-induced gene silencing (VIGS) is an optimal tool for functional analysis of genes in plants, as the viral vector spreads throughout the plant and causes reduced expression of ...
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Tomato and Tobacco Phytoene Desaturase Gene Silencing by Virus-Induced Gene Silencing (VIGS) Technique
... Aims: Virus-Induced Gene Silencing (VIGS) is a virus vector technology that exploits antiviral defense ...(PDS) gene silencing, using VIGS technique by VIGS vector ...
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Establishment of Virus Induced Gene Silencing (VIGS) System in Perennial Rosa Plants under Field Conditions
... Virus-induced gene silencing (VIGS) technique, which is developed in recent years, is a rapid identification of plant gene function from reverse ...post-transcriptional gene ...
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Knock-down the expression of TaH2B-7D using virus-induced gene silencing reduces wheat drought tolerance
... Virus-induced gene silencing (VIGS) is an efficient post-transcriptional gene silencing (PTGS)-based tech- nique for gene functional study ...weak gene ...
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A virus induced gene silencing method to study soybean cyst nematode parasitism in Glycine max
... Virus-induced gene silencing (VIGS) is a reverse genetic tool that exploits RNA-mediated antiviral defense strat- egies used by plants ...during virus infection ...RNA Induced ...
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Efficient virus-induced gene silencing in apple, pear and Japanese pear using Apple latent spherical virus vectors
... Virus-induced gene silencing (VIGS) of endogenous genes in the seedlings of apple, pear, and Japanese pear Because the sequences of widely used VIGS markers, phy- toene desaturase ( PDS ) and ...
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The presence of tomato leaf curl Kerala virus AC3 protein enhances viral DNA replication and modulates virus induced gene silencing mechanism in tomato plants
... Kerala virus-[India:Kerala II:2005] (DQ85263) AC3 protein in the viral life ...and gene-silencing are the two important phenomena that determine the progress of viral infection, we have chosen to ...
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Virus induced gene silencing (VIGS) for functional anaylsis of wheat genes involved in Zymoseptoria tritici susceptibiblity and resistance
... the gene content and composition of all 21 wheat chromosomes identified and annotated 124,201 gene loci (IWGSC, ...laborious. Virus-induced gene silencing (VIGS) has emerged in ...
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Cassava geminivirus agroclones for virus-induced gene silencing in cassava leaves and roots
... a Virus‑Induced Gene Silencing (VIGS) vector and an agroinoculation protocol for gene silencing in cassava (Manihot esculenta Crantz) leaves and ...mosaic virus isolate ...
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Protocol: using virus-induced gene silencing to study the arbuscular mycorrhizal symbiosis in Pisum sativum
... Virus-induced gene silencing (VIGS) exploits a natural defence mechanism in plants against virus infection ...[1]. Virus replication leads to the formation of double- stranded ...
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Elucidating the role of highly homologous Nicotiana benthamiana ubiquitin E2 gene family members in plant immunity through an improved virus-induced gene silencing approach
... recombinant virus carrying a partial sequence from a host gene, the RNAi-mediated anti- viral mechanism can be utilized to silence the corre- sponding host gene, a technique that has been defined as ...
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A rapid virus induced gene silencing (VIGS) method for assessing resistance and susceptibility to cassava mosaic disease
... for virus elimination in infected cassava plants to allow movement of plants between countries or within a country, in vitro germplasm preservation and production of transgenic and gene edited plants often ...
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Virus-induced gene silencing as a tool for functional analyses in the emerging model plant Aquilegia (columbine, Ranunculaceae)
... Leaves were scored for phenotype 5–6 weeks post infiltra- tion. Leaves exhibiting a range of silencing phenotypes were collected and flash frozen at -80°C. Tissue was col- lected from a total of 96 plants ...
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Method: low-cost delivery of the cotton leaf crumple virus-induced gene silencing system
... Figure 1 Details of the CLCrV-derived VIGS vectors described in this paper. A, B) Maps of CLCrV plasmids for biolistic inoculation. C,D) Maps of the CLCrV:CP- (C) and CLCrVB (D) binary vectors for agroinoculation. The ...
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Harnessing anthocyanin-rich fruit: a visible reporter for tracing virus-induced gene silencing in pepper fruit
... maintain silencing for a long period and enhance silencing ...An2 silencing using TRV2-LIC systems clearly resulted in anthocyanin deficiency in leaves, flowers, and ...the gene expression ...
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Optimized cDNA libraries for virus-induced gene silencing (VIGS) using tobacco rattle virus
... Based on the optimal properties for VIGS inserts defined by the above experiments, we developed a cDNA library synthesis method suitable for constructing VIGS libraries. This method, which is outlined in Figure 4, ...
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Characterizing virus-induced gene silencing at the cellular level with in situ multimodal imaging
... pds silencing using the FoMV vec- tor developed by Mei et ...of gene silencing at the cellular level using any VIGS virus targeting genes that produce a unique biochemical signature within ...
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Virus-induced gene silencing of putative Diuraphis noxia (Kurdjumov) resistance genes in wheat
... BSMV viral RNA and BSMV:PDS viral RNA was constructed and inoculated into plants. Five Dnx plants were inoculated with BSMV on the third leaf. The silenced leaf was enclosed in a plastic straw cage and infested with ...
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Virus-induced gene silencing (VIGS) in Cannabis sativa L.
... We aimed for a straightforward method to study the function of genes in C. sativa. Therefore, we established a VIGS system utilizing the Cotton leaf crumple virus (CLCrV) [6]. To visualize the knockdown, we used ...
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