VLP, virus like particle
Advances and challenges in enveloped virus-like particle (VLP)-based vaccines
... Virus-like particles (VLPs) are highly organized particles that self-assemble from viral structural proteins. Like parental viruses, VLPs can be either non-enveloped or enveloped and can be produced ...
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Retrovirus-Based Virus-Like Particle Immunogenicity and Its Modulation by Toll-Like Receptor Activation
... and DCs were isolated from spleens using magnetic separation with CD11c-specific microbeads (Miltenyi Biotec, Paris, France). The purity of the sorted cells was verified by flow cytometry, and the cells were frozen in ...
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A novel polyethyleneimine-coated adeno-associated virus-like particle formulation for efficient siRNA delivery in breast cancer therapy: preparation and in vitro analysis
... used an insect/baculovirus expression system to produce AAV2-VLPs. As shown in Figure 1, the AAV2 cap gene was previously constructed into a baculovirus vector (denoted as BAC-cap) under a polyhedrin promoter for high ...
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Immunogenicity and safety of virus like particle of the porcine encephalomyocarditis virus in pig
... the VLP, two doses of crude protein including VLPs were tested, with the higher dose ...a VLP vaccine can effectively induce neutralizing ...this VLP vaccine for efficiency, safety, and effects on ...
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Development of Virus-Like-Particle Vaccine and Reporter Assay for Zika Virus
... Zika virus (ZIKV) infection and the lack of an approved vaccine raise serious concerns regarding preparedness to combat this emerging ...a virus-like particle (VLP)-based approach to ...
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A Virus-Like Particle-Based Epstein-Barr Virus Vaccine
... from VLP-immunized mice but not mice immu- nized with exosomes from HEK293 cells revealed strong im- munoreactivity against the selected viral proteins, which are components of virions of EBV ...from ...
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An Intranasal Virus Like Particle Vaccine Broadly Protects Mice from Multiple Subtypes of Influenza A Virus
... For the ELISAs, Maxisorp ELISA plates (Sigma-Aldrich, St. Louis, MO) were coated with 2 g/ml H1 or H7 VLP and blocked in 1% bovine serum albumin (BSA) in PBS for 90 min each at 37°C. Plates were then incubated ...
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Structure and Assembly of a T=1 Virus-Like Particle in BK Polyomavirus
... We can here conclude that both Ca 2⫹ and disulfide links are important for the stability of the recombinant BKV T ⫽ 7 cap- sid. However, when the ionic strength of the buffer solution is increased, hydrophobic effects ...
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A Virus-Like Particle System Identifies the Endonuclease Domain of Crimean-Congo Hemorrhagic Fever Virus
... Generation of CCHF virus-like particles expressing a reporter gene. For our attempts to generate tc-VLPs for CCHFV, we built on the previously established minireplicon system (14). The orig- inal ...
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A Virus-Like Particle That Elicits Cross-Reactive Antibodies to the Conserved Stem of Influenza Virus Hemagglutinin
... influenza virus A- ...FHV particle surface, residues 206 to 208 and residues 264 to 268 ...after particle assembly, flexible polypeptide termini in close proximity after the loops are removed, and ...
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A Leucine Residue in the C Terminus of Human Parainfluenza Virus Type 3 Matrix Protein Is Essential for Efficient Virus-Like Particle and Virion Release
... for VLP production (33, 34). We indeed found several L domain-like se- quences within the HPIV3 M protein by analyzing the amino acid sequence and constructed several mutants with deletions (54- YLDV-57, ...
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Enhanced Influenza Virus-Like Particle Vaccination with a Structurally Optimized RIG-I Agonist as Adjuvant
... Immunization. BALB/c mice (6 to 8 weeks of age, Jackson Laborato- ries) were housed in cage units, fed ad libitum, and cared for under USDA guidelines for laboratory animals. For immunization, mice were anesthe- tized ...
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Therapeutic immunisation of rabbits with cottontail rabbit papillomavirus (CRPV) virus like particles (VLP) induces regression of established papillomas
... 14. Villa LL, Costa RL, Petta CA, Andrade RP, Ault KA, Giuliano AR, Wheeler CM, Koutsky LA, Malm C, Lehtinen M, Skjeldestad FE, Ols- son SE, Steinwall M, Brown DR, Kurman RJ, Ronnett BM, Stoler MH, Ferenczy A, Harper DM, ...
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“Development of Prophylactic Bivalent and Quadrivalent Human Papillomavirus (HPV) Vaccines: A Review” by Vikrant C. Sangar, Balasaheb B. Ghongane, India.
... After designing vaccine candidate, it was necessary to develop in vitro neutralization assays that could critically evaluate the antibody responses. Till 1990, there were only two assays were present for measuring ...
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Divergent Bro1 Domains Share the Capacity To Bind Human Immunodeficiency Virus Type 1 Nucleocapsid and To Enhance Virus-Like Particle Production
... down two forms of HA-Brox that were separable by 10% SDS-PAGE. These likely corresponded to farnesylated and unmodified Brox, because the latter form is known to exhibit retarded migration in SDS-PAGE (23). NC did not ...
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Prevalence of antibodies to human papillomavirus (HPV) type 16 virus-like particles in relation to cervical HPV infection among college women.
... (HPV-16) virus-like particle (VLP)-based enzyme-linked immunosorbent assay (ELISA) was used to measure serum antibody to capsid proteins in 376 sexually active college women who were also ...
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Structural analysis and insertion study reveal the ideal sites for surface displaying foreign peptides on a betanodavirus-like particle
... Sea bass immunization and antibody response detection Healthy Asian sea bass juveniles with an average body weight of 5 g were maintained in flow-through freshwa- ter at room temperature, aerated with an air pump and ...
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Papillomavirus capsid binding and uptake by cells from different tissues and species.
... investigated virus-cell interactions by the following two methods: (i) using purified bovine PV virions or human PV type 11 (HPV type 11) virus-like particles (VLP) to test the binding to ...
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In Vitro Assembly of Virus-Like Particles of a Gammaretrovirus, the Murine Leukemia Virus XMRV
... Immature retroviral particles are assembled by self-association of the structural polyprotein precursor Gag. During maturation the Gag polyprotein is proteolytically cleaved, yielding mature structural proteins, matrix ...
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In vivo antiviral efficacy of prenylation inhibitors against hepatitis delta virus
... It is interesting to note that the antiviral strategy we have outlined represents a paradigm different from classical antiviral approaches, since we are not actu- ally directly targeting a viral protein. Rather, the ...
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