Voltage-gated ion channel
Cloning and functional expression of voltage gated ion channel subunits from cnidocytes of the Portuguese Man O'War Physalia physalis
... with 2.5·mmol·l –1 sodium pyruvate, 100·U·ml –1 penicillin, 100· g·ml –1 streptomycin and 5% horse serum. For transcription, the full-length construct of the  subunit was ligated into an oocyte expression vector ...
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Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment
... hypothetical protein hypothetical protein CE8 ATP-binding Cassette ABC superfamily AA10 threonine aspartase hypothetical protein voltage-gated Ion Channel VIC superfamily hypothetical pr[r] ...
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Evolution of voltage gated ion channels at the emergence of Metazoa
... the voltage-gated ion channel superfamily are found in all Domains of life, suggesting that these channels are extremely ancient, possibly as ancient as the last common ancestor of all ...
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Selectivity Mechanism of the Voltage-gated Proton Channel, H V
... tition between the native proton and its rivals, were treated explicitly using density functional theory to account for electronic effects such as polarization of the participating entities and differential amounts of ...
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Regulation of voltage-gated sodium channel expression in cancer : hormones, growth factors and auto-regulation
... bovine adrenal chromaffin cells, insulin increased cell-surface expression of VGSC (Nav1.7) and b1 via PI3K; the tran- scriptional pathway involved phosphorylation of glycogen synthase kinase-3b (GSK-3b), and ...
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In Vivo Evidence for Voltage-Gated Sodium Channel Expression in Carcinomas and Potentiation of Metastasis
... on voltage-gated ion channels (VGICs) due to the profound impact upon cellular processes of membrane potentials (equivalent to some 10,000,000 V / ...suggests voltage-gated sodium ...
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Structural characterisation of recombinant and synthetic voltage-gated potassium channel domains
... (e.g. ion channels) often consist o f a-helical stretches aligned in parallel with the lipid molecules o f the bilayer, the polar ends o f the helices on or around the membrane surfaces on either ...
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Block of T-type calcium channels by protoxins I and II
... ProTx I is both a potent and selective blocker of hCav3.1 ProTx I belongs to the inhibitory cysteine knot (ICK) fam- ily of peptide toxins that are known to interact with voltage-gated ion channels ...
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Molecular basis of ion permeability in a voltage gated sodium channel
... the channel (Appendix Fig S7A), again consistent with the electrophysiology ...monovalent ion commonly found in biological systems, the exclusion of only larger ions is an effective strategy for producing ...
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pH regulation and beyond: unanticipated functions for the voltage-gated proton channel, HVCN1
... other ion channels is evident ...the channel is perfectly selective [31,32], which suggests that protons permeate proton channels by a hydrogen- bonded chain mechanism [33] that does not require a ...
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The role of disulfide bond replacements in analogues of the Tarantula toxin ProTx II and their effects on inhibition of the voltage gated sodium ion channel Nav1 7
... We thank Eisai for a Ph.D. studentship (to Z.V.F.W.), the Wellcome Trust for a studentship (to S.M., 109073/Z/15/Z), the EPSRC for a studentship (to R.D.), and BBSRC/Waters for a CASE studentship (to A.C.). The G2Si ...
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A chemical-scale study on the ligand-binding site of a serotonin-gated ion channel
... Electrophysiological recordings were made 24–72 hours after injection. Whole- cell currents from stage VI oocytes of Xenopus laevis were measured in standard two- electrode voltage clamp mode using the OpusXpress ...
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Detailed comparison of expressed and native voltage-gated proton channel currents
... of voltage-gated proton currents (Thomas & Meech, 1982), genes for voltage-gated proton channels were identified in humans (Ramsey et ...other voltage-gated ion ...
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Designing a C84 fullerene as a specific voltage gated sodium channel blocker
... of ion channel families and are rapidly de- graded in vivo , converting these toxins to drugs represents a considerable challenge, and attempts are being made to synthesize smaller and more durable mimetic ...
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Molecular dynamics of ion transport through the open conformation of a bacterial voltage gated sodium channel
... NavMs voltage dependence of activation and inactivation is >50 mV more hypopolarized than NaChBac and ...NavMs channel requires more en- ergy in the form of a hypolarized membrane potential to stay ...
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Identification of Mutation in SCN4A, Hot-Spot for Periodic Paralyses, in a Large Chinese Family with a Typical Normokalemic PP Using the Whole-Exome Sequencing
... a-subunit, voltage-gated sodium channel, ...that ion permeation through the gating pore is controlled dynamically by the state of the voltage sensor and by rotamer conformations of R4 ...
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Clinical, genetic and expression studies of human CNS channelopathies
... possible combinations e.g. one mutant subunit + three wild type, two mutant + two wild type, three mutant + one wild type, four m utant and four wild type. Even if a complete channel contains three mutant and one ...
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Perspective The gp91 phox Component of NADPH Oxidase Is Not a Voltage-gated Proton Channel
... Because voltage-gated proton channels are ex- pressed by control HEK-293 cells (Bánfi et ...express voltage-gated proton channels (Maturana et ...
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Ion channels contribute to the regulation of cell sheet forces during Drosophila dorsal closure
... to acute changes during closure. For example, at the level of apical junctions, individual AS cells change shape dramatically, whereas the overall area of the AS decreases slowly and monotonically (Hutson et al., 2003; ...
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The comprehensive analysis of DEG/ENaC subunits in Hydrareveals a large variety of peptide-gated channels, potentially involved in neuromuscular transmission
... For six of the thirteen heterotrimers, which are activated by RFamides in the oocyte expression system, the three subunits are co-expressed (Figure 5). It is therefore likely that these six heterotrimers form ...
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