Western blot assay.
Evaluation of a Commercial Echinococcus Western Blot Assay for Serological Follow-Up of Patients with Alveolar Echinococcosis
... Echinococcus Western Blot IgG ...remaining Western blot pattern difficult or even ...commercial Western blot assay had demonstrated the disap- pearance of bands at 16, 18, ...
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Development of a Western Blot Assay for Detection of Antibodies against Coronavirus Causing Severe Acute Respiratory Syndrome
... our Western blot ...our Western blot assay and an IFA with auto- immune disease samples: this set comprised eight samples, in- cluding the four samples mentioned above, which were ...
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Comparison of the western blot assay with the neutralizing antibody and enzyme linked immunosorbent assays for measuring antibody to verocytotoxin 1
... VT1-NAb assay and the ELISA is that only a relatively small proportion of patients infected with VTEC isolates that express VT1 develop detectable VT1- NAb or anti-VT1 IgG, as measured by ELISA or by VT1-NAb ...
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<p>Implications of the Receptor Tyrosine Kinase Axl in Gastric Cancer Progression</p>
... lines. Western blot assay showed that the expression of Gas6, Axl, and p-Axl was upregulated in four selected GC cell lines, including MGC- 803, BGC-823, AGS, and SGC-7901 cells (Figure ...PCR ...
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<p>Long Noncoding RNA CCAT1 Functions as a Competing Endogenous RNA to Upregulate ITGA9 by Sponging MiR-296-3p in Melanoma</p>
... by Western blot ...MTT assay suggested that the upregulation of ITGA9 abol- ished the inhibition impact on cell proliferation caused by miR-296-3p (Figure 6K and ...
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Expression of TTV ORF2 Protein for Detection of Anti TTV IgG Antibodies in Human Sera
... from patient with liver disease, 50 sera from renal dis- eases and 10 sera from healthy controls were analyzed for anti-TTV antibodies. These samples were first ana- lyzed for the presence of TTV-DNA by PCR. Analysis of ...
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SNHG 6 promotes the progression of Colon and Rectal adenocarcinoma via miR-101-3p and Wnt/β-catenin signaling pathway
... activity assay and western blot assay, the activity of Wnt/β-catenin signaling and the protein level of β-catenin and c-Myc (the downstream tar- get genes of the Wnt/β-catenin signaling ...
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<p>Long noncoding RNA STXBP5-AS1 inhibits cell proliferation, migration, and invasion through inhibiting the PI3K/AKT signaling pathway in gastric cancer cells</p>
... and Western blot assay, we found that in the pcDNA STXBP5-AS1 group, STXBP5 expression was lower than the pcDNA ...by Western blot assay results, indicating that p-AKT1 level was ...
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Hepatitis B Virus Core Protein Dephosphorylation Occurs during Pregenomic RNA Encapsidation
... a Western blot assay that can resolve HBV core proteins into hyper- and hypophosphorylated species and obtained evidence support- ing the notion that core protein dephosphorylation occurs in pgRNA ...
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Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays
... the Western blot is still considered the “gold stan- dard” for type-specific serological diagnosis of HSV, the cum- bersome nature of the assay, along with the unavailability of a commercial ...
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Effect of Immunization with Recombinant OspA on Serologic Tests for Lyme Borreliosis
... immunosorbent assay (ELISA), an in-house-developed Western blot assay, two commercial Western blot tests, and a commercially available dot blot ...from Western ...
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Interlaboratory Optimization and Evaluation of a Serological Assay for Diagnosis of Human Baylisascariasis
... rBpRAG1 Western blot assay greatly improves laboratory diagnosis of ...rBpRAG1 assay eliminates the need for additional testing to rule out toxocariasis and increases the confidence in a ...
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Mutant Human Cytomegalovirus Lacking the Immediate-Early TRS1 Coding Region Exhibits a Late Defect
... (B) Western blot analysis of pIRS1 and pTRS1 expression after infection at a multiplicity of infection of 5 PFU/cell with ADwt or ...(Left) Western blot assay of pTRS1 expression after ...
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Histone deacetylase inhibitor sodium butyrate suppresses proliferation and promotes apoptosis in osteosarcoma cells by regulation of the MDM2–p53 signaling
... Abstract: Histone deacetylase inhibitors have been reported to induce tumor cell growth arrest, differentiation, and apoptosis. This study aimed to investigate the effects of one histone deacetylase inhibitor – sodium ...
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Monoclonal antibodies to P24 and P61 immunodominant antigens from Nocardia brasiliensis.
... Nocardia brasiliensis is a gram-positive bacterium, related to mycolic acid-containing actinomycetes like the genera Myco- bacterium, Corynebacterium, Gordona, etc. (5, 10, 17), that lives in the soil as a saprophyte. N. ...
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<p>Intersectin 1 (ITSN1) identified by comprehensive bioinformatic analysis and experimental validation as a key candidate biological target in breast cancer</p>
... used Western blot assay to verify Ki67 and cleaved caspase-3 protein levels in breast cancer cells transfected with siRNA-ITSN1 or the ITSN1-overexpres- sing ...
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Evaluation of a Treponema pallidum western immunoblot assay as a confirmatory test for syphilis
... The results shown in Table 3 indicate that the Western blot assay and FTA-ABS DS were in close agreement, except for the following discordant results: the Western blot assay detected all[r] ...
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Evaluation of a Western Blot Method for the Detection of Yersinia Antibodies: Evidence of Serological Cross-Reactivity between Yersinia Outer Membrane Proteins and Borrelia burgdorferi
... by Western blot assay, and 10% of these were positive for Yersinia antibodies by the agglutination ...the Western blot assays and ELISAs that use Yop antigens that are specific for ...
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Serological Diagnosis of Human Herpes Simplex Virus Type 1 and 2 Infections by Luciferase Immunoprecipitation System Assay
... The Western blot assay for gG is con- sidered the “gold standard” in HSV detection and can discrim- inate between HSV-1 and HSV-2 infections, but this method is time-consuming and less quantitative ...
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Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans
... reason why AdV-VRGB was not used for all 4 immunizations was to prevent the mice from dying due to the injection of adenovirus. To measure the titer of anti-VR IgG, ELISA using the purified GtfB as the antigen and goat ...
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