Western blots
Serodiagnosis of Lyme borreliosis by Borrelia burgdorferi sensu stricto, B garinii, and B afzelii western blots (immunoblots)
... borreliosis Western blots does affect the level of reactivity detected with specific European ...borreliosis Western blot sero- positivity, and therefore criteria must be carefully validated for ...
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Interpretation criteria for standardized Western blots for three European species of Borrelia burgdorferi sensu lato
... Lyme borreliosis (LB) is a global tick-borne disease caused by infection with Borrelia burgdorferi sensu lato. The disorder develops in stages and with different manifestations involving mainly the skin, the nervous ...
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Validity of Interpretation Criteria for Standardized Western Blots (Immunoblots) for Serodiagnosis of Lyme Borreliosis Based on Sera Collected throughout Europe
... of Western blots and evaluation of various interpretation ...The blots were assessed blindly and, to minimize any variation in interpretation, were always assessed by the same ...
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Evaluation of Two Commercial Systems for Automated Processing, Reading, and Interpretation of Lyme Borreliosis Western Blots
... The diagnosis of Lyme borreliosis (LB) is commonly made by serologic testing with Western blot (WB) analysis serving as an important supplemental assay. Although specific, the interpretation of WBs for diag- nosis ...
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Aberrant Phex function in osteoblasts and osteocytes alone underlies murine X linked hypophosphatemia
... Consistent with these observations, analysis of Western blots revealed that the aberrant Pi transport in Cre-PhexΔflox/y and OC-CrePhexΔflox/y mice, like that in the hyp-mice, was due to[r] ...
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Loss of nuclear PTEN in HCV-infected human hepatocytes
... [22,23]. Western blots of HCV-infected hepatocytes showed increased γ-H2AX levels as compared to uninfected hepatocytes (Figure 6A, second lane from the ...
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Genetic and Molecular In Vivo Analysis of Herpes Simplex Virus Assembly in Murine Visual System Neurons
... used Western blots and antibodies to VP5 and gD, markers of the capsid and envelope subassemblies, respectively, to determine that the viral proteins were synthesized and transported in the ganglion cell ...
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Three major glycoprotein genes of varicella-zoster virus whose products have neutralization epitopes.
... To characterize further the specificities of the monoclonal antibodies, both purified virus and infected cell extracts were denatured and resolved on Western blots, and ascites fluids we[r] ...
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Neutralizing Antibodies Protect against Lethal Flavivirus Challenge but Allow for the Development of Active Humoral Immunity to a Nonstructural Virus Protein
... in Western blots, cannot be formally ruled out, a comparison of these sera with an E-monospecific TBEV hyperimmune serum (ELISA titer, 1:180 6 100 versus 1:46,280 6 11,090; NT, negative versus 1:983 6 126; ...
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Glucose transport and its' regulation in rodent mammary gland
... Results described in Sections 4.2-4 .6 had indicated th at ra t mammary gland expressed a putative homologue of the human/rat GLUTl glucose transporter. However they had not definitively established th at the protein ...
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Complex transcription of the utrophin gene: A study of short utrophin isoforms
... identification of a short N-terminal utrophin mRNA and protein by Zuellig et al. (2000). Zuellig and colleagues describe a 3.7Kb transcript encoding a 62kDa protein detected in Northern and Western blots ...
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Inhibition of cyclin E1 sensitizes hepatocellular carcinoma cells to regorafenib by mcl-1 suppression
... c The cell viability of Huh7 cells transfected with the control or CCNE1 plasmid and treated with different concentrations of regorafenib or sorafenib.. The western blots and flow cytome[r] ...
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A sixth Epstein-Barr virus nuclear protein (EBNA3B) is expressed in latently infected growth-transformed lymphocytes.
... Since EBNA3A and EBNA3C are encoded by two of three structurally similar and distantly homologous genes and are two of the three proteins similar in size detected on Western blots immuno[r] ...
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Mycoplasma suis Antigens Recognized during Humoral Immune Response in Experimentally Infected Pigs
... Figure 4 shows representative kinetics of the antibody re- sponse obtained by ELISA. M. suis antibodies were not found in any of the sera taken at housing and prior to inoculation. After experimental infection, M. ...
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AMP activated protein kinase (AMPK) in the rock crab, Cancer irroratus: an early indicator of temperature stress
... Representative western blots and the respective quantification for phosphorylated and therefore activated AMPK (p-AMPK), heat shock protein 70 (HSP70 inducible and total) and the loading control actin, for ...
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Biodistribution of gadolinium- and near infrared-labeled human umbilical cord mesenchymal stromal cell-derived exosomes in tumor bearing mice
... Dot blots and western blots of mesenchymal stromal cell (MSC) exosomes. A) Dot blots showing the expression of tetraspanins: clusters of differentiation (CD) 9, CD63 and CD81, heat sho[r] ...
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Clinical and Pathological Heterogeneity of Korean Patients with CAPN3 Mutations
... brachii (F5b, F6, and F8). Frozen muscle sections (5-μm thick- ness) from all muscle specimens were stained with hematoxy- lin and eosin, modified Gomori trichrome, and reduced nico- tinamide adenine ...
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Integrin adhesion molecules in the human endometrium Correlation with the normal and abnormal menstrual cycle
... To further demonstrate that immunohistochemical staining accurately reflected changes in the expression of 13 subunit on endometrial epithelium, immunoblots Western blots were performed [r] ...
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Isolation of Recombinant Antibodies against EspA and Intimin of Escherichia coli O157:H7
... ELISA, Western blot, and dot blot (Table 2 and ...dot blots, but not all were functional in Western blots and none bound to their cognate epitope in ...in Western blots and dot ...
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Production and characterization of monoclonal antibodies specific for lipooligosaccharide of Serpulina hyodysenteriae
... on Western blots (Fig. 1C) (the Western blot for MAb 9C5 is not shown), and produced strong fluorescence on intact spiro- chetes in the ...by Western blotting ...
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