Western immunoblotting
Western Immunoblotting for Bartonella Endocarditis
... combined, Western immunoblotting and cross-adsorption provide a highly sensitive (96%) diagnostic ...and Western blot analysis are powerful tools not only for establishing an etiological diagnosis of ...
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Detection of anti-Borrelia burgdorferi antibody responses with the borreliacidal antibody test, indirect fluorescent-antibody assay performed by flow cytometry, and western immunoblotting.
... by Western immunoblotting and the flow cytometric assay for indirect fluorescent antibody? Individual ...the Western immunoblotting response. Western immunoblotting also detects ...
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Differentiation of Bartonella Species by a Microimmunofluorescence Assay, Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis, and Western Immunoblotting
... The genus Bartonella presently contains 14 species (3, 12, 16, 17, 24): Bartonella bacilliformis, B. henselae, B. quintana, B. vinsonii, B. elizabethae, B. talpae, B. peromysci, B. grahamii, B. taylorii, B. doshiae, B. ...
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Comparison of Western Immunoblotting and the C6 Lyme Antibody Test for Laboratory Detection of Lyme Disease
... disease Western immunoblotting (WB) kits and the C6 Borrelia burgdorferi (Lyme) enzyme-linked immunosorbent assay (ELISA) kit were compared using two commercially available perfor- mance panels from the ...
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Western immunoblotting analysis of the antibody responses of patients with human monocytotropic ehrlichiosis to different strains of Ehrlichia chaffeensis and Ehrlichia canis.
... tive 120-kDa protein in 17 HME patients’ sera (3, 19). It is presumed that the explanation is the apparently smaller quan- tity of this protein relative to those of proteins in the 44- to 88-kDa range and proteins in the ...
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Comparison of five commercial enzyme linked immunosorbent assays and Western immunoblotting for human immunodeficiency virus antibody detection in serum samples from Central Africa
... Samples which were repeatedly positive by ELISA for HIV-1 and HIV-2 and which were negative or indeterminate by HIV-1 Western blot were analyzed by HIV-2 Western blot DuPont.. These samp[r] ...
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Detection of Antibodies to Anaplasma phagocytophilum and Ehrlichia chaffeensis Antigens in Sera of Korean Patients by Western Immunoblotting and Indirect Immunofluorescence Assays
... cells (Tables 1 and 2). When purified A. phagocytophilum an- tigen was used, all 24 serum specimens reacted with an approx- imately 44-kDa antigen, presumably major surface protein 2 (Msp2). The reaction patterns of ...
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Cross reacting antigens between Neorickettsia helminthoeca and Ehrlichia species, shown by immunofluorescence and Western immunoblotting
... sennetsu produced antisera which reacted with the antigens common to these two Ehrlichia species but not shared with the rest of the members of the tribe Ehrlichieae.. When rabbits or mi[r] ...
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Comparison of Western immunoblotting and microimmunofluorescence for diagnosis of Mediterranean spotted fever
... When the specificity of the results of our tests using healthy blood donors is evaluated true negative/[true negative + false positive], it may be concluded that the WB assay is more spe[r] ...
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Quantitative western immunoblotting analysis in survey of human immunodeficiency virus seropositive patients
... The profiles obtained corroborate the p24 and gp4l antibody titers measured with second-generation ELISA; they confirm the predictive value of the p24 antibody decrease for the progressi[r] ...
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Western Immunoblotting with Five Treponema pallidum Recombinant Antigens for Serologic Diagnosis of Syphilis
... Five immunodominant Treponema pallidum recombinant polypeptides (rTpN47, rTmpA, rTpN37, rTpN17, and rTpN15) were blotted onto strips, and 450 sera (200 from blood donors, 200 from syphilis patients, and 50 potentially ...
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Identification of Giardia lamblia specific antigens in infected human and gerbil feces by western immunoblotting
... Water-soluble antigen from feces collected over a 30-day period from a second group of gerbils, infected this time with the H-3 isolate, revealed a similar set of stepladder bands on day[r] ...
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Development of natural immunity in Plasmodium falciparum malaria: study of antibody response by Western immunoblotting
... Two different blood-stage antigens exoantigens and somatic antigens were used to analyze the distribution of different classes and subclasses of immunoglobulins according to the age of t[r] ...
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Effects of diet-induced obesity on protein expression in insulin signaling pathways of skeletal muscle in male Wistar rats
... Western immunoblotting was carried out on the four experimental groups (chow-fed, vehicle, diet-to-chow, and fenofibrate-treated) to measure protein expression levels, ie, PKC-[r] ...
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Evaluation of bioMérieux's Dissociated Vidas Lyme IgM II and IgG II as a First Tier Diagnostic Assay for Lyme Disease
... IgM Western immunoblot assay ...by Western immunoblotting was performed; 57% of the LYM-positive healthy control samples (from areas where Lyme disease is endemic and where it is nonen- demic) were ...
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Comparison of Mycoplasma arthritidis strains by enzyme linked immunosorbent assay, immunoblotting, and DNA restriction analysis
... two-dimensional Western immunoblotting showed that strains 158p10 and 158p10p9 were no longer antigenically closely re- lated to their parent strain, strain 158 ...
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Identification of the Epstein-Barr virus terminal protein gene products in latently infected lymphocytes.
... A panel of Burkitt's lymphoma cell lines and cell lines established with virus recovered from the BL cells were analyzed by Western immunoblotting and found to contain the 53-kDa TP1 pro[r] ...
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Unexpected transcellular protein crossover occurs during canonical DNA transfection.
... performed western immunoblotting on the supernatant alone which showed no immunoreactive SNAP25 nor SNAP25 D 9 while resuspended EGFP-LcA transfected cells clearly did (Supplementary ...
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Genetic and Antigenic Diversities of Major Immunoreactive Proteins in Globally Distributed Ehrlichia canis Strains
... by Western immunoblotting using sera from naturally infected dogs from each country, and four major immunoreactive proteins (gp19, gp36, gp140, and gp200) were identified in each strain using ...
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α1 and β adrenoceptor stimulation differentially activate p38 MAPK and atrial natriuretic peptide production in the perfused amphibian heart
... p38-MAPK phosphorylation was assessed by western immunoblotting in samples 100 µg of protein from control Rana ridibunda hearts selective antagonist, 1 µmol l–1 both abolished the and he[r] ...
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