whole-cell patch
Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus
... Whole-cell patch-clamp experiments were done as de- scribed before ...double patch-clamp EPC10 amplifier and PatchMaster software (HEKA ...The patch pipettes were pulled from ...
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Specific Inhibition of the Plasmodial Surface Anion Channel by Dantrolene
... the whole-cell patch- clamp configuration. The whole-cell configuration not only permits detection of PSAC in isotonic salt solutions, but it avoids concerns about conclusions based on ...
12
Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
... Whole-cell patch-clamp technique was employed to detect the effects of the egg extract on the ion channels in rat dorsal root ganglion (DRG) ...The patch- clamp pipettes with a tip resistance ...
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Characterization of voltage gated ionic currents in a peripheral sensory neuron in larval Drosophila
... To provide a baseline for future studies of sensory neuron function and development in Drosophila, we have devel- oped a method for performing whole-cell patch clamp recordings from sensory neurons ...
7
Taurodeoxycholate activates potassium and chloride conductances via an IP3 mediated release of calcium from intracellular stores in a colonic cell line (T84)
... We used perforated and standard whole-cell patch-clamp techniques, and the intracellular Ca2+ indicator fura-2 to investigate the mechanism of TDC' stimulation of ionic conductances in t[r] ...
10
Cloning, expression and identification of KTX-Sp4, a selective Kv1.3 peptidic blocker from Scorpiops pococki
... Whole-cell patch-clamp recordings were performed using an EPC 9 amplifier (HEKA Elektronik, Lambre- cht/Pfalz, Germany) at room temperature (22–24 ...
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GAL-021 and GAL-160 are Efficacious in Rat Models of Obstructive and Central Sleep Apnea and Inhibit BKCa in Isolated Rat Carotid Body Glomus Cells
... 1°C). Whole cell patch clamp recordings were then obtained from type I cells in voltage clamp ...mode. Patch pipettes had resistances of 4- 6M ...the whole cell configuration ...
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Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.
... The development of safe technologies to genetically modify neurons is of high interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily ...
13
Solenodon genome reveals convergent evolution of venom in eulipotyphlan mammals
... Representative whole-cell patch-clamp traces showing human muscle-type TE671 (left) and locust neuron nAChR (right) responses to 10 µM acetylcholine and the co-application of acetylcho[r] ...
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Developmental changes in spinal neuronal properties, motor network configuration and neuromodulation at free swimming stages of Xenopus tadpoles
... NEW & NOTEWORTHY A novel in vitro brainstem/spinal cord preparation is described that enables whole cell patch-clamp recordings from spinal neurons in pro-metamorphic Xenopus tadpoles.. [r] ...
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Immunosuppressive effects of a novel potassium channel toxin Ktx-Sp2 from Scorpiops Pocoki
... engineering. Whole-cell patch-clamp experiment showed that Ktx-Sp2 peptide could effectively block three types of exogenous voltage-gated potassium chan- ...the cell model, this study found ...
8
High glutamate permeability and distal localization of Best1 channel in CA1 hippocampal astrocyte
... A whole-cell patch clamp was made onto the astrocytes expressing scrambled- or Best1-shRNA (identified by co-staining of SR-101 and GFP (co-expressed with shRNA)) using the pipette solution ...
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The influence of 8-prenylnaringenin on the activity of voltage-gated kv1.3 potassium channels in human jurkat t cells
... the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated ...lymphocyte cell line ...cancer cell lines expressing ...
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Acid sensing ion channels: potential therapeutic targets for neurologic diseases
... 1). Whole-cell patch clamp recording studies show that an increase in intracellular Ca 2+ due to cerebral ischemia may be attenuated by the nonspecific Na + channel inhibitor amiloride or the spe- ...
8
Purinergic and cholinergic modulation of spinal motor networks in mice
... bath applied to spinal cord slice preparations while performing whole-cell patch-clamp recordings of ventral horn interneurons. Despite being a heterogeneous population of interneurons, responses to ...
139
High production of ectoine from aspartate and glycerol by use of whole-cell biocatalysis in recombinant Escherichia coli
... For the identification of intracellular ectoine, cells were harvested by centrifugation and freeze-dried. Approxi- mately 20 mg of cell material was extracted according to the method of Bligh and Dyer [32]. The ...
10
The Band Notch Sensitivity of Vivaldi Antenna Towards CSRRs
... frequency range [21]. Different size of CSRRs have been etched on the radiation patch in order to achieve the desired notching band and we can clearly observe from Fig. 7, the center frequency of the notched band ...
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Differences in Antibody Response to Whole-Cell Pertussis Vaccines
... However, differences in reaction rates to whole-cell diphtheria-tetanus-pertussis vaccines have been cited by several studies, suggesting that whole-cell vaccines are not consistent prod[r] ...
7
Uniform Analytic Scattered Fields of a PEC Plate Illuminated by a Vector Paraxial Gaussian Beam
... The integration domain S corresponds to a rectangular patch. In order to get well known double canonical integrals, we rewrite the integration domain S as the difference between the whole[r] ...
15
Target Mechanism Based Whole Cell Screening Identifies Bortezomib as an Inhibitor of Caseinolytic Protease in Mycobacteria
... than whole-cell ap- proaches, to identify molecules that interact with purified target proteins derived by ...with whole-cell activity, either because of cell permeability issues that ...
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