Xeno free
Are serum-free and xeno-free culture conditions ideal for large scale clinical grade expansion of Wharton’s jelly derived mesenchymal stem cells? A comparative study
... able xeno-free and serum-free media, StemPro XF, SFM (Invitrogen, Carlsbad, CA, USA) and MesenCult XF, SFM (Stem Cell Technologies, Vancouver, British Columbia, Canada), to establish and expand ...in ...
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Human mesenchymal stem cells maintain their phenotype, multipotentiality, and genetic stability when cultured using a defined xeno-free human plasma fraction
... other xeno-free and serum-free media than in FBS-supplemented media; how- ever, usually the genetic stability was not studied and only hMSCs from one origin were used [12, 13, ...
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Simple production method of umbilical cord derived mesenchymal stem cell using xeno free materials for translational research
... Previous studies isolated MSCs from UC tissue using enzymatic processings [5, 6] or explant method that was harvested once [7, 8, 9]. We have developed a multiple harvest method, which can harvest the explant more than ...
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Enhanced xeno-free differentiation of hiPSC-derived astroglia applied in a blood–brain barrier model
... and xeno-free conditions is desirable, and a detailed understanding of how specific laminin isoforms affect both the differentiation and maturation of astroglia is essential for in vitro applications in ...
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Top-Down Inhibition of BMP Signaling Enables Robust Induction of hPSCs Into Neural Crest in Fully Defined, Xeno-free Conditions
... and xeno-free differentiation medium that can be used to generate putative neural crest from hPSCs in a robust ...fully xeno-free produc- tion of neural crest for clinical applications and for ...
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Amenable epigenetic traits of dental pulp stem cells underlie high capability of xeno free episomal reprogramming
... In addition, DPSCs were found to exhibit a higher feeder-free reprogramming capability. The feeder inde- pendence of human cell sources was previously noted for ASCs because this cell type can support self-renewal ...
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Comparative analysis of human mesenchymal stem cells from bone marrow and adipose tissue under xeno-free conditions for cell therapy
... Cell-based therapies need viable and ample numbers of cells for regenerative medicine. In this study, we aim to optimize MSC culture under xeno-free conditions and scale up MSCs in preparation for potential ...
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Improved GMP compliant approach to manipulate lipoaspirates, to cryopreserve stromal vascular fraction, and to expand adipose stem cells in xeno-free media
... compared to nonexpanded ASC contained in the SVF, CD34 expression was blunted in expanded ASC and the expression level of CD13, CD105, CD29, and CD166 was increased (Table 1, part B). Under our ex- perimental conditions, ...
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Optimization of human mesenchymal stem cell manufacturing: the effects of animal/xeno-free media
... Alternative media formulations consisted of DMEM supplemented with 10%HPL (BMMSC- 10%HPL, and AdMSC-10%HPL) and a commercially available serum-free/xeno-free culture medium (BMMSC-SFM/XF, ...
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Isolation, characterization, and gene expression analysis of Wharton’s jelly-derived mesenchymal stem cells under xeno-free culture conditions
... cell-specific marker profile between the two populations of the WJ-MSCs and to investigate whether there is a shift in the propensity to differentiate towards a particular lineage on switching from FBS to allogeneic HS, ...
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Clinical outcomes of xeno-free expansion and transplantation of autologous ocular surface epithelial stem cells via contact lens delivery: a prospective case series
... for xeno-free expansion to meet the increasingly strict regulatory ...for xeno-free culture and expansion and as a carrier for ocular surface SC transplant- ation in 16 patients with ...
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Culture of human mesenchymal stem cells using a candidate pharmaceutical grade xeno-free cell culture supplement derived from industrial human plasma pools
... -produced, xeno-free, pharmaceutical-grade, human plasma-derived supplement for cell culture (SCC) is under development at Grifols (Parets del Vallès, Barcelona, ...
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Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium
... suitable for clinical applications. Thus, we used DKSFM, which is a medium with no fibroblast feeder layers, as a xeno-free culture condition for culturing keratinocytes in the present study. Furthermore, ...
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Human adipose tissue-derived stem cells cultured in xeno-free culture condition enhance c-MYC expression increasing proliferation but bypassing spontaneous cell transformation
... In addition to the risk posed by the use of FBS in the ex vivo expansion of hASCs for clinical purposes, spon- taneous malignant transformation in vitro is another po- tential risk [17]. Some groups have reported that ...
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Biological impact of xeno-free chemically defined cryopreservation medium on amniotic epithelial cells
... commercial xeno-free cryomedia, proposed for stem cell cryopreservation, were selected; CryoStor CS10, CryoStor CS5 (BioLife), STEM- CELLBANKER (amsbio), CryoStem (Stemgent), and Synth-a-Freeze (Life ...
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Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions
... cells. Xeno reagents would not only increase the risk of infections but also cause immune rejection upon cell transplantation ...use Xeno-free reagents in all cell handling ...
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Human embryonic stem cell cultivation: historical perspective and evolution of xeno-free culture systems
... under xeno-free conditions in defined medium supplemented with lam- inin, fibronectin, bFGF, Activin A and neurotrophins BDNF, NT3 and ...scalable xeno-free and clinically compliant culture ...
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GMP-compatible and xeno-free cultivation of mesenchymal progenitors derived from human-induced pluripotent stem cells
... under xeno-free conditions and according to Good Manufacturing Practice (GMP) ...in xeno-free and GMP-compatible ...with xeno-free alternatives or use of GMP-compatible media ...
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Comparison of a xeno-free and serum-free culture system for human embryonic stem cells with conventional culture systems
... Another obstacle to overcome was the presence of FBS or SR in the culture medium. Several studies de- scribed the use of HS as serum supplement to culture hESCs. However, it has been proved previously that commercial ...
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Scalable efficient expansion of mesenchymal stem cells in xeno free media using commercially available reagents
... Here we present data using a commercially avail- able platelet-lysate based media, XcytePLUS™, to culture human umbilical cord’s Wharton’s Jelly MSC (WJ-MSC) in comparison to media containing FCS. Additionally, the ...
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