Xenopus oocyte
Differential role of STIM1 and STIM2 during transient inward (Tin) current generation and the maturation process in the Xenopus oocyte
... It is well known that when Ca 2+ is released from the ER, STIM proteins are activated, rapidly translocated, and oligomerized into junctions formed between the ER and the plasma membrane, where they bind to and acti- ...
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Xenopus oocyte maturation: cytoplasm alkalization is involved in germinal vesicle migration
... IlIt, I, Dev,lIilll ~O ~71 ~76 (1996) Original Article Xenopus oocyte maturation cytoplasm alkalization IS involved in germinal vesicle migration STEPHANE FLAMENT', EDITH BROWAEYS', JEAN LUC RODEAU2,[.] ...
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Reprogramming of somatic cells and nuclei by Xenopus oocyte and egg extracts
... grown Xenopus oocytes, reaching ...maturation, Xenopus eggs are arrested awaiting fertilization in the metaphase of the second meiotic division due to the high activity of MPF and CSF ...Mature ...
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The roles of maternal Vangl2 and aPKC in Xenopus oocyte and embryo patterning
... Fig. 5. aPKC regulates the distribution of the acetylated microtubule cytoskeleton and Vangl2-VAMP1 islands. (A) Histological section of a follicle-free (collagenase-treated) stage 6 Xenopus oocyte ...
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Spatial arrangement of intra nucleolar rDNA chromatin in amplified Xenopus oocyte nucleoli: structural changes precede the onset of rDNA transcription
... Int J De\" BioI 40 263 272 (1996) 263 Spatial arrangement of intra nucleolar rDNA chromatin in amplified Xenopus oocyte nucleoli structural changes precede the onset of rDNA transcription HERBERT SPRI[.] ...
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TBP2 is a substitute for TBP in Xenopus oocyte transcription
... The Xenopus TBP (pSP64A-xTBP) [15] and TBP2 (pT7TSA- xTBP2) [8] constructs were utilized to generate altered binding specificity mutant versions of these proteins by introducing three point mutations in the ...
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Release from Xenopus oocyte prophase I meiotic arrest is independent of a decrease in cAMP levels or PKA activity
... stimulates oocyte maturation, it was significantly less effective than 10 −5 M P4 ...P4-induced oocyte maturation ...of oocyte maturation by PKI ...induce oocyte maturation, hence bringing ...
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Mutations causing Liddle syndrome reduce sodium dependent downregulation of the epithelial sodium channel in the Xenopus oocyte expression system
... in Xenopus oocytes of the mutated or truncated b subunits with wild-type (WT) rat a and g subunits, and of the truncated g subunit with WT rat a and b subunits, resulted in a marked increase in amiloride-sen- ...
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Inhibition of the Na+/Ca2+ Exchanger NCX1 Expressed in Xenopus Oocyte by Glycyrrhizic Acid and Cyclophylin A
... [22] Chen, Z., Mi, L., Xu, J., Yu, J., Wang, X., Jiang, J., Xing, J., Shang, P., Qian, A., Li, Y., Shaw, P.X., Wang, J., Duan, S., Ding, J., Fan, C., Zhang, Y., Yang, Y., Yu, X., Feng, Q., Li, B., Yao, X, Zhang, Z., Xue, ...
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Chromatin assembly and transcriptional cross talk in Xenopus laevis oocyte and egg extracts
... at 55,000 rpm × 45 min. The clarified middle layer was removed and respun for 30 min, and glycerol was added to 5%, aliquoted, and flash frozen. Xenopus oocyte extracts were prepared from freshly dissected ...
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Expression cloning of a rat hepatic reduced glutathione transporter with canalicular characteristics
... Using the Xenopus oocyte expression system, we have isolated a 4.05-kb cDNA RcGshT from a rat liver library encoding for a polypeptide of 835 amino acids that mediates GSH transport.. In[r] ...
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Acetylcholinesterase and butyrylcholinesterase genes coamplify in primary ovarian carcinomas
... coamplify in leukemias. To examine the relationship of gene amplification to the expression of these genes in tumors, ACHE and CHE genes and their expression were studied in primary ovarian carcinomas. DNA blot ...
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Potential role of protein tyrosine phosphatase nonreceptor type 13 in the control of oocyte meiotic maturation
... in Xenopus oocyte maturation, small interference RNA (siRNA) was used to downregulate the PTPN13 ...available Xenopus PTPN13 ...using Xenopus PTPN13 or Xenopus GAPDH ...the ...
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De Monte, Erica (2017): Time course and pathologies of in vitro maturation and fertilization of bovine oocytes. Dissertation, LMU München: Tierärztliche Fakultät
... the oocyte contributes only for one half of the genome to the future embryo, normal oocyte maturation is considered a prerequisite for successful fertilization, early embryo formation and further ...
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The development of free swimming in Xenopus laevis larvae
... 78 no direct connection was made between the spontaneous recordings and the behaviour, despite their use of EMG recordings. What they did do however was to perform mechanical lesions on their isolated larval preparation ...
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... Immediately upon arrival in the lab, ovaries were freed of surrounding tissues, washed in Washing Medium (WM) which consisted of Medium 199 with Earle´s salts (M4530) supplemented with 3 mg/mL bovine serum albumin (BSA), ...
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Freezing the Biological Clock: A Viable Fertility Preservation Option for Young Singaporean Women?
... [elective oocyte cryopreservation] are not convincing” ...towards oocyte cryopreservation has been more ...merited oocyte cryopreservation’s declassification as an “experimental procedure”,17 it ...
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Primary ovarian insufficiency: an update
... with oocyte retrieval followed by oocyte or embryo cryopreservation, ovarian tissue cryopreservation, or ovarian suppression with a gonadotropin-releasing hormone ...
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The amphibian oocyte nucleus
... than animals which had been treated with gonadotrophin#. I tried to assess this difference by measuring the total volume of sphere material per oocyte nucleus in each animal before and after treatment. For a given ...
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Lighting up mRNA localization in Drosophila oogenesis
... the oocyte, consistent with kinesin-dependent transport (see Movie 3 in the supplementary ...the oocyte, is decreased in kinesin I mutants with reduced motor speed but not in dynein mutants, it is unlikely ...
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