Y-27632
Failure of Y-27632 to improve the culture of adult human adipose-derived stem cells
... continuous Y-27632 supplementation (C/Y); 5 days of growth with Y-27632 fol- lowed by growth in control conditions (Y/C); and 10 days of growth with Y-27632 ...
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Rho kinase inhibitor Y-27632 promotes the differentiation of human bone marrow mesenchymal stem cells into keratinocyte-like cells in xeno-free conditioned medium
... (Stemcell Technologies, Vancouver, BC, Canada), which is a defined serum- and xeno-free medium. MesenCult- SF (Stemcell Technologies) attachment substrate was used to pre-coat the culture plates for 2 hours at room ...
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Original Article Rho-associated protein kinase inhibitor Y-27632 increases the sensitivity of lung adenocarcinoma A549 cells to matrine via the Rad51/ERCC1 signaling pathway
... cancer. Y-27632 is widely used in the treatment of cardiovascular disease, inflam- mation, and cancer ...Although Y-27632 inhibits the growth and invasion of NSCLC cells (such as H1299 and ...
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Y-27632 is associated with corticosteroid-potentiated control of pulmonary remodeling and inflammation in guinea pigs with chronic allergic inflammation
... ovalbumin, Y-27632 after the fifth inhalation, and corticosteroid treatment; OVA: Animals that received ovalbumin; OVA-C: Animals that received ovalbumin and corticosteroids; OVA-RHO: Animals that received ...
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Modulation of human uterine smooth muscle cell collagen contractility by thrombin, Y-27632, TNF alpha and indomethacin
... events through via activation of the protease activated receptors, it is proposed that F2R is the main receptor responsible for mediating these responses [8]. It had pre- viously been demonstrated that thrombin is ...
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The Rho-Associated Kinase Inhibitor Fasudil can Replace Y-27632 for Use in Human Pluripotent Stem Cell Research
... This study began with a serendipitous question as to why Y-27632 is the primary choice for PSC studies, despite the low cost of fasudil and its ability to improve hPSC survival. This question arose because ...
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Inhalation of the Rho-kinase inhibitor Y-27632 reverses allergen-induced airway hyperresponsiveness after the early and late asthmatic reaction
... when Y-27632 was given intranasally prior to allergen challenge, pulmonary eosinophilia was reduced, as shown by a decreased number of eosinophils in the bron- choalveolar lavage (BAL) fluid ...administered ...
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Rho- Kinase Inhibitor Y-27632 Decreases the Thickness of Trabecular Meshwork in Juvenile Rats Mod-el Injected with Sodium Hyaluronate
... inhibitor Y-27632 on the thickness of trabecular meshwork in juvenile rats model injected with sodium ...topical Y- 27632 10 mM, and three experimental groups with intracameral injection of ...
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ROCK inhibitor, Y-27632, reduces FBS-induced structural alteration in organ-cultured mesenteric artery
... of Y-27632 during organ culture (Huh et ...μM Y-27632 maintained the structural integrity of the smooth muscle cells with intact actin and myosin filaments (Figure ...
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Original Article Effect of the Rho kinase inhibitor Y-27632 and fasudil on inflammation and fibrosis in human mesangial cells (HMCs) under high glucose via the Rho/ROCK signaling pathway
... inhibitor Y-27632 and fasudil on the development of human mesangial cell (HMC) inflammation and fibrosis induced by high glucose and to clarify the contribution of the Rho/ROCK signaling pathway in the ...
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Y-27632 enhances differentiation of blastocyst like cystic human embryoid bodies to endocrinologically active trophoblast cells on a biomimetic platform
... However, expression of IGF2 a characteristic of trophoblast cells was seen to be stronger in the treated as compared to the con- trol (faint band). The expression of -actin was simi[r] ...
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Use of a Rho kinase inhibitor to increase human tonsil keratinocyte longevity for three-dimensional, tissue engineered tonsil epithelium equivalents
... Figure 6: Streptococcus pyogenes-infected tonsil epithelium equivalents display increased expression of pro-inflammatory cytokines compared to untreated controls. Tissue engineered tonsil epithelium equivalents generated ...
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Rock1 Inhibitor Abrogates Chronic Rejection in Rat Cardiac Model System
... the Y-27632, a highly selective, commercially available, inhibitor of Rho-associated pro- tein kinase p160ROCK (ROCK1) [32,33], to abrogate chronic rejection in rat cardiac ...
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Macroscopic stiffening of embryonic tissues via microtubules, RhoGEF and the assembly of contractile bundles of actomyosin
... RhoGEF activated RhoGTPase could influence tissue stiffness by altering F-actin polymerization (Watanabe et al., 1999), by enhancing the bundling of filaments (Machesky and Hall, 1997), or by enhancing myosin II ...
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ROCK inhibitor reduces Myc-induced apoptosis and mediates immortalization of human keratinocytes
... Lastly, we queried which apoptotic pathway was inhibited by Y-27632. Apoptosis or programmed cell death can be triggered by two major pathways within the cells: the extrinsic pathway (death receptor ...
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RhoA/ROCK Signaling Regulates TGFβ-Induced Epithelial-Mesenchymal Transition of Lens Epithelial Cells through MRTF-A
... Transforming growth factor (TGF)-β–induced epithelial-mesenchymal transition (EMT) leads to the formation of ocular fibrotic pathologies, such as anterior subcapsular cataract and posterior capsule opacification. ...
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The effect of Rho kinase inhibition on long-term keratinocyte proliferation is rapid and conditional
... of Y-27632 on keratinocyte prolif- eration could be due to a continual selection for a small subset of proliferative, stem-like cells or it could be due to a conversion of the entire culture population to a ...
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Oncogenic role of dysregulated leptin signaling in the pathogenesis of ovarian cancer
... and Y-27632) inhibited the invasiveness of ovarian cancer cells and leptin-induced upA expression [50] This il- lustrates not only the involvement of JAK/STAT and PI3K/ AKT signaling pathways but other ...
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ROCK inhibition promotes microtentacles that enhance reattachment of breast cancer cells
... after Y-27632 treatment has been previously reported, we did not observe any significant change in acetylation of alpha-tubulin or tau phosphorylation upon ROCK inhibition in the breast cancer cells tested ...
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rcmb.2004-0009oc.pdf
... of Y-27632 on Neutrophil Cytoskeleton Human neutrophils were isolated from peripheral blood of healthy volunteers using a two-step density gradient of Histopaque 1077 and 1119 (Sigma Chemicals) as ...
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