Y lipolytica
Gene repression via multiplex gRNA strategy in Y. lipolytica
... for Y. lipolytica now allows enhanced homologous recombina- tion efficiency without labored genetic knockouts and promises to be a potent tool for other metabolic engi- neering ...
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Evaluation of single cell oil (SCO) from a tropical marine yeast Yarrowia lipolytica NCIM 3589 as a potential feedstock for biodiesel
... As expected, an easily utilizable carbon source such as glucose, affected the variation in lipid yields and FAME profiles among the different Y. lipolytica strains. It was noted that on an optimal glucose ...
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3, 4-dihydroxy-L-phenylalanine-derived melanin from Yarrowia lipolytica mediates the synthesis of silver and gold nanostructures
... product (quinone form) via a semi-quinone state [21]. Such oxidation-reduction reactions involving phenolic compounds are known to mediate nanoparticle synthesis in other biological systems [22]. Taking into account ...
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Identification, characterization of two NADPH-dependent erythrose reductases in the yeast Yarrowia lipolytica and improvement of erythritol productivity using metabolic engineering
... Yarrowia lipolytica, the last step of erythritol synthesis involves the reduction of erythrose by specific erythrose ...yeast Y. lipolytica MK1 was identified (Janek et ...in Y. ...
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Substrates and oxygen dependent citric acid production by Yarrowia lipolytica: insights through transcriptome and fluxome analyses
... including Y. lipolytica, nitrogen limitation gradually change the metabolism from cell growth phase to citric acid production and/or storage lipid accumulation phase ...engineered Y. ...
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Lipid production by the oleaginous yeast Yarrowia lipolytica using industrial by-products under different culture conditions
... Fed‑batch cultures subject to different oxygenation conditions and initiated with different inoculum densities Studies examining lipid production by Y. lipolytica using fed-batch or repeated-batch cultures ...
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Hydrolysis of carotenoid esters from Tagetes erecta by the action of lipases from Yarrowia lipolytica
... engineered Y. lipolytica has been applied in fermentations where omega-3 eicosa- pentaenoic acid, a fatty acid with a wide range of health benefits, is produced by carefully balancing expression levels of ...
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Enhanced protopanaxadiol production from xylose by engineered Yarrowia lipolytica
... Yarrowia lipolytica contains the native mevalonate (MVA) pathway to provide precursor compounds, namely isopentenyl pyrophosphate and dimethylallyl pyrophosphate [18], indicating that ...Y. ...
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Advances and opportunities in gene editing and gene regulation technology for Yarrowia lipolytica
... in Y. lipolytica in the near ...in Y. lipolytica as they function in mammalian ...for Y. lipolytica; however, it would be a useful tool for making specific edits directly in the ...
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A novel multigene expression construct for modification of glycerol metabolism in Yarrowia lipolytica
... with Y. lipolytica ACA-YC 5033 strain (2 g L -1 , 30% of DCW, even though the culture was carried out in shake flasks) [64] and ...Y. lipolytica LGAM S(7)1 ...in Y. lipolytica ...
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Engineering of a high lipid producing Yarrowia lipolytica strain
... native Y. lipolytica DGA1 and DGA2 enzymes have significantly increased lipid yield and productivity [19, 21, 26], and it has been hypothesized that efficient diacylglycerol acyltransferase activity creates ...
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Engineering global transcription to tune lipophilic properties in Yarrowia lipolytica
... years, Y. lipolytica attracts more attention as this yeast produces high-level lipids and its gene manipu- lation is available ...in Y. lipolytica, for example, for carotenoid ...for Y. ...
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Optimization of odd chain fatty acid production by Yarrowia lipolytica
... 50.72 and 20.26%, respectively. Only 1.75% of odd chain fatty acids in total fatty acids were produced in this con- dition. However, in case of medium without glucose like YNBP1 and YNBP2, the ratio of odd chain fatty ...
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Engineering Yarrowia lipolytica to produce biodiesel from raw starch
... So far, metabolic engineering has already boosted lipid production in this yeast. Different target genes for overexpressions and deletions have been identified and manipulated to increase total fatty acid content. As an ...
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Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2
... a Y. lipolytica hybrid promoter created by Madzak et ...the Y. lipolytica strong constitutive glyceraldehyde-3-phosphate dehydrogenase promoter, (iii) TEF1, the ...Y. lipolytica ...
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Metabolic engineering of oleaginous yeast Yarrowia lipolytica for limonene overproduction
... The gene IDI1 (Fig. 1) encodes for an isopentenyl diphos- phate isomerase that catalyzes an essential step in the sterol pathway, the isomerization of IPP to DMAPP. A number of works focused on the overexpression of IDI ...
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Developing cellulolytic Yarrowia lipolytica as a platform for the production of valuable products in consolidated bioprocessing of cellulose
... For fatty acid production on cellulose, CYLpO con- sumed 50 g/L of cellulose and produced 5.0 g/L FAME in 96 h (Fig. 4c). Continuous cellulase production was observed for CYLpO grown on glucose and this was accompanied ...
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Engineering the oleaginous yeast Yarrowia lipolytica for production of α-farnesene
... from Y. lipolytica was placed under the control of the hp4d promoter, and the HMG-CoA reductase gene (HMGR, GenBank: Bpet3342) from ...into Y. lipolytica strain PO1f, and integrated into the ...
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Genetic Control of Extracellular Protease Synthesis in the Yeast Yarrowia lipolytica
... Yarrowia lipolytica secretes an acidic protease or an alkaline protease, the synthesis of which is also controlled by carbon, nitrogen, and sulfur availability, as well as by the presence of extracellular ...in ...
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Development of a novel rDNA based plasmid for enhanced cell surface display on Yarrowia lipolytica
... of Y. lipolytica, its encoding sequence containing an enterokinase cleavage site at its C-terminal was fused to the N-terminal of the YlCWP1 encoding ...into Y. lipolytica Po1f using the ...
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