yeast 2-hybrid system
Identification of Proteins That Interact with Podocin Using the Yeast 2 Hybrid System
... The podocin bait (called pGBKT7-podocin) used in the yeast 2- hybrid system contained the full-length cDNA fused in-frame with the GAL4 DNA-binding domain of pGBKT7. The pGBKT7-podocin was ...
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Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2
... DNA binding assay. We examined the DNA binding activity of each RBP-J mutant by electrophoresis mobility shift assay (EMSA) essentially as described previously (3) except that in vitro-translated mutant proteins rather ...
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Target validation of the inosine monophosphate dehydrogenase (IMPDH) gene in Cryptosporidium using Phylomer® peptides
... The yeast two-hybrid assay is a system for identifying and analysing protein–protein interactions in vivo, which has provid- ed insight into many of the protein networks that constitute biological ...
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Original Article Construction of human colon cancer stem cells cDNA library and identification of novel proteins interacted with stratifin
... into yeast strain AH109, yeast two-hybrid screening assay was performed by mating AH109 with Y187 that contained colon cancer stem cells cDNA library ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... The yeast cells were ready for trans- ...pared yeast cells, 7 μg cDNA library or library vector pPR3-N and finally ...ml 2×YPAD before repeating the ...
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Characterization of cDNAs from Nicotiana benthamiana that encode proteins which interact with tomato golden mosaic virus AL2 protein in the yeast two-hybrid system
... It has been previously determined that AL2 protein in TGMV is a multifunctional protein. TGMV AL2 protein was initially found to be a viral trans-activator (Sunter and Bisaro, 1997). It de-represses the AR1 promoter in ...
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The identification of a novel protein interacting with GABAA receptors using the yeast two-hybrid system
... y-Aminobutyric acid^ (GABA^) receptors are members o f the ligand-gated ion channel superfamily. They are hetero-oligomeric proteins which are composed o f varying combinations o f subunits to form functional receptors. ...
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Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation
... immediate-early 2 (IE2) protein of human herpesvirus 6 is a potent transactivator of cellular and viral ...the yeast two-hybrid system, a Jurkat T-cell cDNA library for proteins that could ...
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In the present A work on selective breeding aimed at further consolidation of key qualities of the race of Zalaajinst Edren white goats towards increased length and fineness of fibers and upgrading phenotypic characteristics (Dorjbat Yo, 2013) resulted i
... using yeast as a model ...the yeast two-hybrid system and the potential physiological importance of such an interaction, we proceeded to analyze the biochemical properties of these proteins in ...
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Non-erythroid beta spectrin interacting proteins and their effects on spectrin tetramerization
... gene 2" (residues 1-478), a fragment (residues 74-442) of septin 8 isoform c, a fragment (residues 704-953) of "coatomer protein complex, subunit beta 1, a fragment (residues 146-614) of zinc-finger ...
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Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two hybrid system
... Y2H system, Y2HGold cells containing the pGBKT7-TgGRA15 plasmid were used to mate with Y187 cells harboring the Mate & Plate™ Universal Mouse (Normalized) cDNA ...
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Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome
... In order to evaluate how well our new vectors prevented false negatives we compared our data to published data. First, we used a literature-curated dataset of herpesviral- interactions [4]. Since not many VZV ...
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Identification of a gp41 Core-Binding Molecule with Homologous Sequence of Human TNNI3K-Like Protein as a Novel Human Immunodeficiency Virus Type 1 Entry Inhibitor
... a yeast two-hybrid screening on a human bone marrow cDNA library using the recombinant soluble gp41 ectodomain as the bait and identified a novel gp41 core-binding molecule, designated ...
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The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism
... triggering visual neuronal response [27]. After that, all- trans-retinaldehyde is reduced into all-trans-retinol by re- ductases generally, stored in retinal pigment epithelium (RPE) and then transformed into ...
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Interaction of the Cauliflower Mosaic Virus Coat Protein with the Pregenomic RNA Leader
... FIG. 3. (A) Schematic representation of the CaMV ORF IV-encoded pro- tein (pIV; coat protein) and the N- and C-terminal deletion mutants under study. Two acidic regions (ac), the basic region (basic), the nuclear ...
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Frontiers of Functional Genomics (FFG)
... Figure 2 shows an overview of the major areas of functional genomics which come into the scope of the programme, illustrating their integration through bioin- formatics and systems biology, and indicating that ...
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Human cytomegalovirus capsid assembly protein precursor (pUL80.5) interacts with itself and with the major capsid protein (pUL86) through two different domains.
... Plasmid construction. Standard techniques were used to construct, clone, and propagate plasmids (69). All constructs were verified by DNA sequence analysis. (i) Subcloning of viral genes into the two-hybrid ...
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Evaluation of Interactions of Human Cytomegalovirus Immediate-Early IE2 Regulatory Protein with Small Ubiquitin-Like Modifiers and Their Conjugation Enzyme Ubc9
... in yeast two- hybrid ...in yeast two- hybrid assays led to our working hypothesis that IE2 may associate with several cellular proteins through binding to co- valently conjugated SUMO moieties ...
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Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair.
... lane 2, lysate from BSVprXThy-pXCR23A cotransfected cells (30 ml) incubated with GSTVpr; lane 3, lysate from BSVprXThy-pXCR23A cotransfected cells (30 ml) incubated with ...
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Yeast two-hybrid: State of the art
... the yeast PLM is currently under ...like yeast with approximately 7000 proteins becomes not trivial since, false positives and negatives might seriously interfere with subsequent ...
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