Yeast One-Hybrid System
Identification and Characterization of Human Genomic Binding Sites for Retinoic Acid Receptor/Retinoid X Receptor Heterodimers
... modified yeast one-hybrid system ...modified one-hybrid assay were constructed by inserting eight copies of the mouse cellular retinoic acid binding protein II (mCRABPII) RARE ...
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Target validation of the inosine monophosphate dehydrogenase (IMPDH) gene in Cryptosporidium using Phylomer® peptides
... a yeast-two-hybrid system was used to identify Phylomer peptides within a library constructed from the genomes of 25 phylogenetically diverse bacteria that targeted the IMPDH of Cryptosporidium ...
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In Search of Regulators of LeSPL CNR by South Western Blotting and Yeast One Hybrid Library Screening System
... Gene expression regulatory networks are comprised of cis- and trans- acting fac- tors, and differences in gene expression are attributable to genetic variation. Ep- igenetic change can also be considered as an important ...
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Characterization of cDNAs from Nicotiana benthamiana that encode proteins which interact with tomato golden mosaic virus AL2 protein in the yeast two-hybrid system
... used. One approach is to use an immunocapture ...from yeast strains that express TAL2(W124A)-LexA and B42AD fused with Nb#51 or Nb#62 would be passed over the anti-LexA ...in yeast would initiate ...
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Identification of Proteins That Interact with Podocin Using the Yeast 2 Hybrid System
... A frozen mouse kidney tissue sample (100 mg) was homogeniz- ed in TRIZOL reagent (Gibco, Carlsbad, CA, USA), and total RNA was then isolated by addition of a mixture containing chloroform, isopropyl alcohol and 75% ...
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OsSND2, a NAC family transcription factor, is involved in secondary cell wall biosynthesis through regulating MYBs expression in rice
... through yeast one-hybrid screening using the secondary wall NAC-binding element (SNBE) on the promoter region of OsMYB61 which is known transcription factor for regulation of SCWs biosynthesis as ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... Several colonies of NMY51 were taken from YPAD plates and then inoculated into 50 ml YPAD and grew overnight at 30°C with shaking. Upon the OD546 of the culture reaching 0.6- 0.8, the culture was centrifuged at 700 g for ...
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The identification of a novel protein interacting with GABAA receptors using the yeast two-hybrid system
... y-Aminobutyric acid^ (GABA^) receptors are members o f the ligand-gated ion channel superfamily. They are hetero-oligomeric proteins which are composed o f varying combinations o f subunits to form functional receptors. ...
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An OTU deubiquitinating enzyme in Eimeria tenella interacts with Eimeria tenella virus RDRP
... Background: Chicken coccidiosis, a disease caused by seven species of Eimeria (Apicomplexa: Coccidia), inflicts severe economic losses on the poultry industry. Eimeria tenella is the one of the most virulent ...
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Yeast two-hybrid: State of the art
... the yeast two-hybrid system are reviewed by ...This yeast strain contains three reporter genes: HIS3, ADE2 and LacZ, each under control of a different promoter (GAL1, GAL2, and GAL7 ...
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Functional Interaction between Human Herpesvirus 6 Immediate-Early 2 Protein and Ubiquitin-Conjugating Enzyme 9 in the Absence of Sumoylation
... Sumoylation is the process of covalently attaching a small ubiquitin-related modifier (SUMO) moiety to a target protein. In humans, at least four SUMO proteins can be found, with 47% amino acid identity between SUMO-1 ...
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Interaction of calcium and integrin binding protein 1 with integrin α11 and its possible involvement in pulmonary fibrosis
... is one of the collagen-binding integrin α - chains [8,9] that is overexpressed in non-small-cell lung carcinoma and regulates insulin-like growth factor-2 expression in fibroblasts ...
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Downstream of tyrosine kinase/docking protein 6, as a novel substrate of tropomyosin-related kinase C receptor, is involved in neurotrophin 3-mediated neurite outgrowth in mouse cortex neurons
... Our previous work has shown that Dok5 is a substrate of tropomyosin-related kinase (Trk)B and TrkC receptors and is involved in neurotrophin-induced MAPK activa- tion [22]. Neurotrophins, including nerve growth factor ...
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The 41-Amino-Acid C-Terminal Region of the Hepatitis E Virus ORF3 Protein Interacts with Bikunin, a Kunitz-Type Serine Protease Inhibitor
... culture system for HEV, funda- mental studies on its replication and expression strategy have not been ...and yeast and in vitro in a coupled transcription-translation system (12, 15, 17, 22, ...
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Functional Replacement of the Intracellular Region of the Notch1 Receptor by Epstein-Barr Virus Nuclear Antigen 2
... FIG. 1. Interaction regions of RBP-J with EBNA2 and RAM23. (A) Positions of the point and insertional mutations in RBP-J (3) are shown by ellipses. N (residues 212 to 227) and C (residues 275 to 323) regions shown by ...
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Rapid and differential regulation of AMPA and kainite receptors at hippocampal mossy fibre synapses by PICK1 and GRIP
... GluR6/7 Ab (Upstate Biotechnologies); (vi) anti-GluR6/7(C18) Ab We thank Michael Hollman and Steve Heinemann for the generous (Santa-Cruz, CA); (vii) anti-bassoon mAb (Stressgen); (viii) anti- gift of KAR clones and Seth ...
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White Spot Syndrome Virus Protein Kinase 1 Defeats the Host Cell's Iron-Withholding Defense Mechanism by Interacting with Host Ferritin
... the yeast two-hybrid ...of yeast (Y187 and AH109) and grown on SD/ ⫺ Leu/ ⫺ Trp (a) and then on agar plates with higher-stringency media, ...of yeast on the two high-stringency plates was ...
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Identification of a gp41 Core-Binding Molecule with Homologous Sequence of Human TNNI3K-Like Protein as a Novel Human Immunodeficiency Virus Type 1 Entry Inhibitor
... a yeast two-hybrid screening assay using the recom- binant soluble gp41 ectodomain (rsgp41e) as the bait in hopes of identifying a novel HIV fusion inhibitor with sequence ho- mology to a human protein and ...
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Interaction of the Cauliflower Mosaic Virus Coat Protein with the Pregenomic RNA Leader
... proteins. Hybrid protein 1 (see ...the yeast strain L40-coat. To obtain hybrid protein 2, the full-length open reading frame IV (ORF IV) (pIV) and its variants (CPs, numbered with the amino acid ...
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The interaction of selenoprotein F (SELENOF) with retinol dehydrogenase 11 (RDH11) implied a role of SELENOF in vitamin A metabolism
... SELENOF As a result, the aldehyde production has been increased and then might probably convert to VR to per- form important roles insome physiological processes. But the mechanism should be studied in the future work.- ...
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