Yeast two-hybrid analysis
Identification of novel ligands of WDR47, using yeast two-hybrid analysis
... the two domains into close proximity to form the transcription ...Y2H analysis the ADE2, HIS3 and MEL1 reporter genes were used as indicators of protein ...AH109 yeast strain, whereas the GAL4 DNA-AD ...
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Yeast two-hybrid: State of the art
... the yeast cells ...for yeast cells that are more difficult to make competent for ...(competent) yeast cells known to be good for transformation. Even by using yeast cells known to have worked ...
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pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... In this paper, we present pISTil, a fully automated pipe- line combined to a web-based interface, which are specif- ically devoted to ISTs identification and analysis. The pISTil system is highly flexible and ...
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Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay.
... these two-hybrid interactions to viral positive-strand RNA replication has not been ...the two-hybrid ...the two-hybrid assay, we have found that the interaction between the ...
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Geminivirus Movement Protein Interactions with Host Proteins in the Yeast Two-Hybrid System
... Rojas, M.R, Jiang, H., Salati, R., Xoconostle-Cazares, B., Sudarshana, M.R., Lucas, W.J., and Gilbertson, R.L. 2001. Functional analysis of proteins invol ved in movement of the monopartite begomovirus tomato ...
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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site
... The ITC methods require not only the preparation of recombinant proteins but also the characterization of these proteins for proper functional analysis. In our systems, we characterized the protein systems with ...
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The identification of a novel protein interacting with GABAA receptors using the yeast two-hybrid system
... the yeast strains CGI 945, Y190 and Y187, verified as far as the methods within the yeast two-hybrid system permit, that one of these clones C3, interacts with the GABA^ receptor ...blot ...
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Identification of host proteins interacting with Toxoplasma gondii GRA15 (TgGRA15) by yeast two hybrid system
... control, two host proteins were identified to interact with ...and analysis of positive prey To determine the identity of positive clones, the two prey plasmids were sequenced using T7 ...the ...
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Improving the yeast two-hybrid system with permutated fusions proteins: the Varicella Zoster Virus interactome
... detailed analysis of VZV ORF10 deletion mutants in SCIDhu skin xenografts revealed that ORF10 is a viru- lence factor for the pathogenesis of VZV in skin ...
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A user-friendly platform for yeast two-hybrid library screening using next generation sequencing
... involves analysis of bait functionality through a Y2H assay with known interactors ...immunoblot analysis can be carried out in checkpoint 1 to validate protein expression of the fusion proteins in case no ...
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Identification of novel protein interactors of the SV40 large T antigen using the yeast two-hybrid system
... FACS analysis was used to show that expression of T antigen in cells treated with nocodazole (a microtubule disrupting agent) makes them more refractory to the spindle assembly ...
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Identification of sensory-neuron-specific sodium channel Nav1.8 interacting proteins by yeast-two hybrid screen
... trap analysis for proteins that regulate the functional expression of voltage gated N a y ...The yeast two-hybrid screen is one method that can be used to identify interactive ...the ...
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Two-hybrid analysis of Ty3 capsid subdomain interactions
... Additional file 1 Sup. Fig. 1. Interactions of BD M4 mutant Gag3 D60A/R63A with Gag3 wild type, D60A/R63A, G87A, F93A, E148A/ K149A, and E190A/R191A. The D60A/R63A mutation disrupts interactions with wild type and other ...
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A Multigene Family That Interacts with the Amino Terminus of Plasmodium MSP-1 Identified Using the Yeast Two-Hybrid System
... the two species) that was transcribed from the opposite strand and was unrelated to the ...contain two small inserts of approximately 20 to 25 amino acids relative to the other molecules, whereas PfM- SRP-5 ...
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PEG-4000 increased the mating efficiency of yeast-two hybrid screening process using PmF-box1 as bait
... Therefore, one modification was conducted to increase the mating efficiency. In this screening, PmF-box1 was used as a bait to screen for the Y2H library which was constructed using RNA from plant samples treated with ...
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Binding Partners of Parvulin Proteins using the High-Throughput Screening Methods such as Yeast two-hybrid and Phage Display
... blot analysis and SDS-PAGE for the protein encoded by MT2A clones Upper panel is a western blot analysis of the protein encoded MT2A clones either from bone marrow (BM) or fetal liver (FL) library followed ...
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Identification of host proteins interacting with the integrin like A domain of Toxoplasma gondii micronemal protein MIC2 by yeast two hybrid screening
... in yeast cells was analyzed by immuno-blotting and their autoactivation was tested via comparison with the pGBKT7 empty vector, which expressed the GAL4 DNA binding-domain ...by ...
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A New Method, “Reverse Yeast Two-Hybrid Array” (RYTHA), Identifies Mutants that Dissociate the Physical Interaction Between Elg1 and Slx5
... Data analysis, fi ltering, and quality assessment Plates were scanned using an HP Scanjet G4010 scanner and converted to JPG images with the resolution of 300 ...
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Improved methods for cloning and detection in the yeast two hybrid assay
... The results in Figure 4 will demonstrate the benefit of the additional rep- lica-plating to SD-Ura-Leu-His plates helps. run on the agarose gel, the circular plasmids will show bands different from the duplicated linear ...
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A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM
... based two-hybrid screening, we reasoned that a protein that interacts with the Gal4-binding domain (Gal4BD) could be used to test the competency of a Gal4BD–bait fusion protein prior to a screen, which ...
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