Yeast two-hybrid assay
Improved methods for cloning and detection in the yeast two hybrid assay
... The results in Figure 4 will demonstrate the benefit of the additional rep- lica-plating to SD-Ura-Leu-His plates helps. run on the agarose gel, the circular plasmids will show bands different from the duplicated linear ...
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Analysis of the interaction of viral RNA replication proteins by using the yeast two-hybrid assay.
... these two-hybrid interactions to viral positive-strand RNA replication has not been ...the two-hybrid ...the two-hybrid assay, we have found that the interaction between ...
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Screening and identification of host proteins interacting with Toxoplasma gondii SAG2 by yeast two hybrid assay
... Results: Yeast two-hybrid (Y2H) experiment was used to identify the binding partners of surface antigens of ...The yeast plasmids for true positive clones were rescued by transformation into ...
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Screening and identification of host proteins interacting with Theileria annulata cysteine proteinase (TaCP) by yeast two hybrid system
... Shuaiyang Zhao 1 , Guiquan Guan 1 , Junlong Liu 1 , Aihong Liu 1 , Youquan Li 1 , Hong Yin 1,2* and Jianxun Luo 1* Abstract Background: Theileria annulata can infect monocytes/macrophages and B lymphocytes and causes ...
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Yeast two-hybrid: State of the art
... to assay the specificity of the interaction can be performed ...the yeast cells ...into two main groups; the ones of potential interest, and the ...
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A Genetic Test for Yeast Two-Hybrid Bait Competency Using RanBPM
... based two-hybrid screening, we reasoned that a protein that interacts with the Gal4-binding domain (Gal4BD) could be used to test the competency of a Gal4BD–bait fusion protein prior to a screen, which ...
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Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... in two hybrid library ...of two or more reporter genes to assay for an interaction has been reported by Fashena et ...classical yeast two hybrid systems relies on the ...
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YEAST two-hybrid and itc studies of alpha and beta spectrin interaction at the tetramerization site
... b yeast AH109 cells were grown in a medium containing all essential amino acids but tryptophan, leucine and histidine, and lacking adenine; c yeast Y187 cells were grown in medium containing all essential ...
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Original Article Screening of APP interaction proteins by DUALmembrane yeast two-hybrid system
... After the co-transformation of pBT3-SUC-APP and its screening products, we observed 8 SD-leu/trp/his/ade/7.5 mM 3-AT plates with white colonies of which the Whatman filter papers turned blue during the β-galactosidase ...
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A user-friendly platform for yeast two-hybrid library screening using next generation sequencing
... In the retained list of potential interactors using threshold SNR TPL-N/EMPTY >6 with FPKM N-TPL >100 values, the one candidate ATCKA2 (AT3G50000) that did not contain an EAR-domain was tested for direct interaction with ...
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Identification of two novel host proteins interacting with Toxoplasma gondii 14 3 3 protein by yeast two hybrid system
... to detect the Tg14-3-3 protein expression, total proteins of Y2HGold cells were extracted and 187. examined by immunoblotting assay using the Myc-tag (9B11) mouse mAb (Cell Signaling)[r] ...
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Identification of novel protein interactors of the SV40 large T antigen using the yeast two-hybrid system
... Expression assay To determine the expression of the baits, cultures were inoculated from the same streaks of the Glu/CM-His-Ura master plate, as for the LEU2 and lacZ ...
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Identification of sensory-neuron-specific sodium channel Nav1.8 interacting proteins by yeast-two hybrid screen
... down assay that the C-terminal of Cx43, the most widely expressed connexin member, binds directly to both a - and P-tubulin. They suggested that Cx43 may function as a microtubule-anchoring protein and thus play a ...
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Novel binding partners for Prenylated Rab Acceptor 1 identified by a split ubiquitin yeast two hybrid screen
... confirmed three interactions using a BiFC assay in cul- tured mammalian cells. Of particular interest within the list of novel binding partners are those that reside in the early secretory path- way. ζ1-COP, a ...
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A Multigene Family That Interacts with the Amino Terminus of Plasmodium MSP-1 Identified Using the Yeast Two-Hybrid System
... the yeast two-hybrid system, we have shown that the carboxy-terminal regions of both ...binding assay that PfMSRP-1 and PfMSRP-2 interact directly with ...
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A New Method, “Reverse Yeast Two-Hybrid Array” (RYTHA), Identifies Mutants that Dissociate the Physical Interaction Between Elg1 and Slx5
... The Yeast Two-Hybrid (Y2H) methodology allows the detection of physical interactions between any two interacting ...Reverse Yeast Two-Hybrid Array ” (RYTHA), that allows ...
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Identification of novel ligands of WDR47, using yeast two-hybrid analysis
... the two domains into close proximity to form the transcription ...AH109 yeast strain, whereas the GAL4 DNA-AD were fused to the proteins within the human foetal cDNA library to form the preys ...
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pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... context, yeast two-hybrid system appears as a promising approach to efficiently reconstruct protein interaction networks at the proteome-wide ...
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Geminivirus Movement Protein Interactions with Host Proteins in the Yeast Two-Hybrid System
... Iwabuchi, K., Li, B., Bartel, P. and Fields, S. 1993. Use of the two-hybrid system to identify the domain of p53 involved in oligomeriztion. Oncogene 8: 1693-1696. Kubo, A. and Kondo, N. 1991. Differences ...
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Genomic Libraries and a Host Strain Designed for Highly Efficient Two-Hybrid Selection in Yeast
... The twehybrid system is a powerful technique for detecting protein-protein interactions that utilizes the well-developed molecular genetics of the yeast Saccharomyces cermisiae.[r] ...
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