Yeast two hybrid screen
Novel binding partners for Prenylated Rab Acceptor 1 identified by a split ubiquitin yeast two hybrid screen
... Prenylated Rab Acceptor 1 (PRA1/Rabac1) is a ubiqui- tously expressed and highly conserved protein, a frag- ment of which was first identified as a Rab6 binding partner in a yeast two-hybrid ...
6
Identification of sensory-neuron-specific sodium channel Nav1.8 interacting proteins by yeast-two hybrid screen
... a yeast two-hybrid ...of two copies of the annexin II heavy chain, also known as calpactin I, lipocortin II, or p36, and two copies of p i ...
174
Phenotypic characterisation and molecular genetic analysis of Leri-Weill dyschondrosteosis
... a yeast two-hybrid screen, since activation domain fiision libraries can be prepared from different organisms, tissues, cell types and developmental ...the yeast ...
279
An OTU deubiquitinating enzyme in Eimeria tenella interacts with Eimeria tenella virus RDRP
... in two clear bands at the expected sizes (1350 bp for RDRP and ...in yeast cells, we extracted total proteins from Y187 cells transformed with pGBKT7-RDRP via the urea/SDS method and analysed them via ...
11
Human Immunodeficiency Virus Type 2 Gag Interacts Specifically with PRP4, a Serine-Threonine Kinase, and Inhibits Phosphorylation of Splicing Factor SF2
... a yeast two-hybrid screen of a T-cell cDNA library to identify cellular proteins that bind to the human immunodeficiency virus type 2 (HIV-2) Gag polyprotein, we identified PRP4, a ...
10
A Nonconventional Nuclear Localization Signal within the UL84 Protein of Human Cytomegalovirus Mediates Nuclear Import via the Importin α/β Pathway
... the yeast two-hybrid screen, indicating a sufficient complexity of the cDNA library and the specificity of the interaction with ...the yeast two-hybrid screen, ...
15
5982.pdf
... expression and 2) the formation of distinct transcriptional complexes with regionally expressed cofactors. We sought to determine if castor zinc finger 1 protein (CASZ1), a novel para zinc finger transcription factor ...
141
Interaction of calcium and integrin binding protein 1 with integrin α11 and its possible involvement in pulmonary fibrosis
... a yeast two-hybrid screen using the cytoplasmic domain of ITGA11 as bait and transformed an EGY48 yeast strain with the bait- containing plasmid using the plasmid from a human lung ...
8
A member of the Plasmodium falciparum PHIST family binds to the erythrocyte cytoskeleton component band 4 1
... Yeast two-hybrid interaction validation and mapping In order to recapitulate the interaction observed in the yeast-two-hybrid screen, a fragment of PF3D7_0402000 was ...
9
Human immunodeficiency virus type 1 Vpr interacts with HHR23A, a cellular protein implicated in nucleotide excision DNA repair.
... vpr gene was ligated to GST sequences in a plasmid that al- lowed expression of a chimeric fusion protein in bacteria. GSTVpr was purified from bacteria by affinity chromatography on glutathione-Sepharose. To produce ...
11
PubMedCentral-PMC5469152.pdf
... a yeast two-hybrid complementation-based screen was performed using XLG protein baits to query interactions between XLG and partner protein found in glucose-treated seedlings, roots, and ...
13
Yeast three hybrid screen identifies TgBRADIN/GRA24 as a negative regulator of Toxoplasma gondii bradyzoite differentiation
... using two sets of primers (Fig. 5A) and sequenced sever- al isoforms. Two isoforms were chosen that encode proteins with different C-terminal ends, TgBRADIN-a and ...Y3H screen with ...
22
A Two-Hybrid Screen of the Yeast Proteome for Hsp90 Interactors Uncovers a Novel Hsp90 Chaperone Requirement in the Activity of a Stress-Activated Mitogen-Activated Protein Kinase, Slt2p (Mpk1p)
... of yeast Hsp82 with a C-terminal Gal4p binding domain (BD) extension (mutant Hsp82-BD fusions) were constructed by a slight modification of the procedure used to make the strains expressing a native, wild-type ...
12
Screening and identification of host proteins interacting with Theileria annulata cysteine proteinase (TaCP) by yeast two hybrid system
... throughput yeast-two-hybrid screening, 7 putative positive clones were ...only two host proteins were identified to inter- act with TaCP after a co-transformation experiment of pGBKT7-TaCP ...
9
Identification of a gp41 Core-Binding Molecule with Homologous Sequence of Human TNNI3K-Like Protein as a Novel Human Immunodeficiency Virus Type 1 Entry Inhibitor
... a yeast two-hybrid system with rsgp41e as the bait to screen a human bone marrow cDNA library, we have identified a novel gp41 core-binding molecule, P20, which is effective in blocking HIV-1 ...
10
In the present A work on selective breeding aimed at further consolidation of key qualities of the race of Zalaajinst Edren white goats towards increased length and fineness of fibers and upgrading phenotypic characteristics (Dorjbat Yo, 2013) resulted i
... functional screen for LeTPx1 interacting ...with two independent isolates 2) LeTPx2, a full length highly homologous Peroxiredoxin with a unique N-terminal extension which likely codes for a chloroplastic ...
9
pISTil: a pipeline for yeast two hybrid Interaction Sequence Tags identification and analysis
... in yeast, "not in frame" ISTs may be more prone to errors related to the irrelevant nature of associated pro- ...Y2H screen (GenBank accession number) and description of cDNA libraries constructions ...
6
Yeast Two Hybrid cDNA Screening of Arabidopsis thaliana for SETH4 Protein Interaction
... Classical Yeast Two-Hybrid System A protein of interest Y is expressed in yeast as a fusion to a DNA- binding domain (DBD, “bait”; circles denote expression ...The two yeast ...
6
The PDZ Proteins PICK1, GRIP and Syntenin bind multiple glutamate receptor subtypes
... searches, yeast two-hybrid assays and glutathione S-transferase (GST)-pull-down approaches we have identified a series of glutamate re- ceptor subunits that interact differentially with the PDZ ...
5
Interactions of AtRGL1, a negative regulator of gibberellic acid signalling : a thesis presented in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry, Massey University, Palmerston North, New Zealand
... The possibility of DELLA protein dimerisation was also investigated using AtRGA, AtRGLI , and AtRGL2 in yeast 2-hybrid ex periments.. Yeast 2-hybrid protein interaction results suggest t[r] ...
13