Zinc binding is important for AvrP recognition
Contribution of the Zinc Finger to Zinc and DNA Binding by a Suppressor of Posttranscriptional Gene Silencing
... the zinc finger were changed to either arginine, asparagine, or ...Another important issue is that such amino acid replacements should impose a minimum effect on the C2 protein ...C2 zinc-finger ...
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Global analysis of Drosophila Cys2-His2 zinc finger proteins reveals a multitude of novel recognition motifs and binding determinants
... CTCF binding site when cloned into our reporter vector activated tran- scription of the reporter genes in the absence of CTCF, likely through the function of an endogenous factor (data not ...the ...
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Characterizing the DNA-Binding Site Specificities of Cis2His2 Zinc Fingers
... The most common linker arrangement found in ZFs contains five residues between the final histidine of one finger and the first conserved aromatic of the next. Roughly half of the known ZFs contain a linker with the ...
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Communication between the zinc and tetrahydrobiopterin binding sites in nitric oxide synthase
... an important second-messenger molecule in neural and cardiovascular systems 4 ...pterin binding site locates right at the dimer interface, therefore, monomeric NOS does not bind H 4 B and substrate 6 ...
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Probing the DNA-Binding Affinity and Specificity of Designed Zinc Finger Proteins
... A simple adaptation incorporating unlabeled oligonucleotide competitors provides a rapid assay for the quantitation of binding specificity. Protein is first added to a solution of labeled probe to near saturation, ...
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Design of Single-Stranded Nucleic Acid Binding Peptides Based on Nucleocapsid CCHC-Box Zinc-Binding Domains
... Guanine recognition is further facilitated by hydrogen bonds involving groups from the polypeptide backbone of the CCHC-box domain and NH1 and O6 of the ...domain binding a guanosine residue, has been ...
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The N-terminal Epidermal Growth Factor-like Domain in Factor IX and Factor X Represents an Important Recognition Motif for Binding to Tissue Factor*
... Factors VII, IX, and X play key roles in blood coagula- tion. Each protein contains an N-terminal ␥-carboxyglu- tamic acid domain, followed by EGF1 and EGF2 do- mains, and the C-terminal serine protease domain. Protein C ...
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Platinum Complexes and Zinc Finger Proteins: From Target Recognition to Fixation
... Though the initial prototype 9EtG compound was successful in affecting the NCp7 in- teraction with viral RNA, it is important to try to improve the characteristics of the drug. Chapters 3 and 4 explored the ...
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An improved predictive recognition model for Cys2-His2 zinc finger proteins
... our recognition model would also allow predic- tion of ZFPs with uncharacterized DNA-binding specifi- city throughout the ...Bcl6 recognition motifs produced by B1H, PBM and SELEX-seq are all similar, ...
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Towards accurate calculations of Zn2+ binding free energies in zinc finger proteins
... For the second method, since the quasi-chemical model treats the outer shell electrostatically using the dielectric constant, a dielectric constant for the zinc finger protein is required. This can be calculated ...
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Solution structure of an archaeal DNA binding protein with an eukaryotic zinc finger fold
... binding of AFV1p06 to DNA: (i) the N- and/or C- disordered termini could participate in the interaction; (ii) another DNA binding protein could bind to AFV1p06 on the b-sheet ...ligand- binding ...
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Severe zinc depletion of escherichia coli: roles for high affinity zinc binding by ZinT, zinc transport and zinc-independent proteins
... yodB b1974 Function unknown 2.38 0.0725 zur b4046 Repressor for znuABC, the zinc high-affinity transport genes; dimer; binds two Zn(II) ions per monomer 1.37 0.9578 znuC b1858 High-affinity ABC transport system ...
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Functional Characterisation of the Flax Rust AvrP/AvrP123 Avirulence Proteins
... 21 examples of devastating, economically-important rusts are white pine blister rust, wheat stem rust, soybean rust and coffee rust. As a group, they are diverse and have a wide host range due to long co-evolution ...
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A zinc-finger like metal binding site in the nucleosome
... conserved zinc finger-like site, composed of Cys110 and His113 residues of both H3 mol- ...the zinc binding ...the binding. Consequences of these findings for zinc homeostasis, metal ...
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... Also, Western blots showed similar amounts of mutant and wild-type LT. Of particular interest for DNA replication is the ability to bind polyomavirus DNA. LT binds specifically to 5 9 -GAGGC- 3 9 -related sequences in ...
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Ebola Virus Transcription Activator VP30 Is a Zinc-Binding Protein
... the zinc-binding do- main of the full-length protein resulted in a complete loss of the transactivating function of VP30 during transcription, pointing to the importance of the integrity of this domain for ...
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Characterization of the Zinc Binding Activity of the Rubella Virus Nonstructural Protease
... cation binding in protease activity, Zn binding studies were performed using the minimal NS protease domain within the NSP ...maltose binding protein (MBP) fusion protein by bacteria, the NS protease ...
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Allosteric Inhibition of Zinc Finger Proteins by DNA Binding Polyamides
... isotherms represent binding at the match target site in the minor groove, which has the sequence shown in the lower right panel of the figure, and which overlaps [r] ...
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Isolation and characterization of zinc-binding proteins of canine seminal plasma
... non zinc-binding proteins (nZnBPs) were isolated by zinc-dependent affinity ...conditions. Zinc-elution profile using affinity chromatography dis- played two protein fractions represented by ...
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Is the gender recognition act 2004 as important as it seems?
... Gender Recognition Act provides a ready means by which a transsexual can seek recognition of his or her new gender and that to rely upon the wide interpretation of A ...Gender Recognition Act, or in ...
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