Zinc Finger Nucleases
Induction of apoptosis in imatinib sensitive and resistant chronic myeloid leukemia cells by efficient disruption of bcr-abl oncogene with zinc finger nucleases
... as zinc finger nucleases (ZFNs) [14–16], transcription acti- vatorlike effector nucleases (TALENs) [17–19] and clus- tered regulatory interspaced short palindromic repeats (CRISPR) / Cas9 [20, ...
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Proviral HIV-genome-wide and pol-gene specific Zinc Finger Nucleases: Usability for targeted HIV gene therapy
... specific zinc finger nucleases, which are proposed for use towards targeted HIV gene ...in zinc finger technology, I have assembled 114 ZFAs (Figure 1, Table 1, and Additional file 2) ...
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Zinc finger nucleases for targeted mutagenesis and repair of the sickle-cell disease mutation: An in-silico study
... the Flavobacterium okeanokoites bacteria restriction endo- nuclease, FokI [33]; have recently become a powerful tool for primarily editing host genomes. Following induction of a double strand break (DSB) within the ...
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Targeted Mutagenesis of Beta Lactoglobulin Gene in Caprine Fetal Fibroblasts by Context Dependent Assembly Zinc Finger Nucleases
... Zinc finger nucleases (ZFNs) are artificial, hybrid restriction enzymes created by covalently linking a DNA- binding zinc finger (Zif) domain (composed of three to six ...
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Zinc Finger Nucleases as tools to understand and treat human diseases
... designer zinc fin- ger proteins has been the ability to cleave specific sites in large ...termed zinc finger nucleases, or ...two zinc finger trimers are bound to their cognate ...
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Identity of zinc finger nucleases with specificity to herpes simplex virus type II genomic DNA: novel HSV-2 vaccine/therapy precursors
... okeanokoites bacteria restriction endonuclease, FokI [34-36]. In-vivo, as noted above, the zinc finger nucleases function as dimers (or pairs) [54-57]. Therefore, considering our three ZF array based ...
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Efficient Gene Targeting in Drosophila With Zinc-Finger Nucleases
... designed zinc-finger nucleases (ZFNs) and production of a linear donor in ...Because zinc fingers can be directed to a broad range of DNA sequences and targeting is very efficient, this ...
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Evaluation and application of modularly assembled zinc finger nucleases in zebrafish
... of zinc fingers recognizing 27 different triplet sequences in the three finger positions of the Zif268 backbone that can be rapidly assembled by PCR into three-finger ZFPs (see Table S1 in the ...
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Targeted Chromosomal Cleavage and Mutagenesis in Drosophila Using Zinc-Finger Nucleases
... yB nucleases ing sites in close proximity (Smith et ...Each finger contacts primarily 3 bp; the crossed with two or three attached-X [C(1)DX] females, and male offspring were screened for yellow body ...
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Targeted Genome Engineering and Its Application in Trait Improvement of Crop Plants
... [56] Shukla, V.K., Doyon, Y., Miller, J.C., DeKelver, R.C., Moehle, E.A., Worde, S.E., Mitchell, J.C., Arnold, N.L., Gopalan, S., Meng, X., Choi, V.M., Rock, J.M., Wu, Y.Y., Katibah, G.E., Gao, Z., McCaskill, D., ...
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Recent developments in synthetic biology and metabolic engineering in microalgae towards biofuel production
... CRISPR: clustered regularly interspaced short palindromic repeats; TALEs: transcription activator-like effectors; ZFN: zinc-finger nucleases; LEA: lipid- extracted algal; RBS: ribosome-[r] ...
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Modified mRNA as a new therapeutic option for pediatric respiratory diseases and hemoglobinopathies
... encoding zinc-finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), and CRISPR-Cas9, have implicated modified mRNA as a very promising option for cancer ...
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Determinants of genome editing outcomes: the impact of target and donor DNA structures
... signer nucleases, scientists were mostly referring to transcription activa- tor-like effector nucleases (TALENs) and zinc-finger nucleases (ZFNs); now RNA-guided nucleases (RGNs) ...
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The therapeutic landscape of HIV-1 via genome editing
... TALENs have not yet been applied for HIV-1 treat- ment in clinical studies. However, several experimen- tal studies have shown promising results with potential for optimization for large scale anti-HIV-1 treatment. Shi ...
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Development of neurodevelopmental disorders: a regulatory mechanism involving bromodomain-containing proteins
... adjacent zinc finger, 1A; BAZ1B: Bromodomain adjacent zinc finger, 1B; BCPs: Bromodomain- containing proteins; Brd1: The first bromodomain protein gene; Brd2: The second Brd protein gene; BRM: ...
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Zinc finger proteins in cancer progression
... C2H2-type zinc finger motif is the largest group of all zinc finger motif ...C2H2-type zinc finger motif is composed of CX2CX3FX5LX2HX3H, and its two cysteine and two histidine ...
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KRAB zinc finger proteins
... for zinc finger arrays; that is, with adjacent KRAB-ZFPs differing by either point mutations at DNA-contacting residues of a few ZNFs or substitutions of entire blocks of these motifs (Kauzlaric et ...
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Structure of Arabidopsis HISTONE DEACETYLASE15
... The zinc finger domain enhances HDA15HD dimerization and enzymatic. 796[r] ...
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Analysis of the interaction between Zinc finger protein 179 (Znf179) and promyelocytic leukemia zinc finger (Plzf)
... kruppel-like zinc fingers ...and zinc-finger proteins, and has been shown to mediate homo/heterodimerization, nuclear localization as well as to direct binding of corepressors ...
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The Zinc Finger Antiviral Protein Directly Binds to Specific Viral mRNAs through the CCCH Zinc Finger Motifs
... The zinc finger antiviral protein (ZAP) was originally recov- ered from a screen for genes conferring resistance to the in- fection of cells by Moloney murine leukemia virus (MLV) ...
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