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A cis-acting function for the coronavirus leader in defective interfering RNA replication.
... To directly test the hypothesis that the leader is sufficient as a 5'-terminal signal for coronavirus RNA replication 18, 43, a clone of BCV N mRNA 40 with a precise 5'-terminal sequence[r] ... See full document
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Importance of the Positive-Strand RNA Secondary Structure of a Murine Coronavirus Defective Interfering RNA Internal Replication Signal in Positive-Strand RNA Synthesis
... single-strand RNA bacteriophage Qb contains two internal viral polymerase rec- ognition sites for negative-strand RNA synthesis; each internal recognition site is separated from its functional initiation ... See full document
8
cis-Acting Sequences Required for Coronavirus Infectious Bronchitis Virus Defective-RNA Replication and Packaging
... for replication and rescue (rescue indicates replication and packaging of the RNA into virus ...M95169). Replication and rescue were determined by quantification of CAT protein by ELISA or, ... See full document
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Replication of murine coronavirus defective interfering RNA from negative-strand transcripts.
... the leader sequence of DE25 ...the leader sequence and the T7 terminator ...the leader sequence and the ribozyme ...the leader, has a part of the HDV ribozyme sequence, and lacks the extra 4 ... See full document
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The UCUAAAC promoter motif is not required for high-frequency leader recombination in bovine coronavirus defective interfering RNA.
... for leader recombination, there- fore, is one in which polymerase strand switching occurs during minus-strand synthesis ...the leader-flanking UCUAAAC sequence and thus downstream of the leader, a ... See full document
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The 3' untranslated region of coronavirus RNA is required for subgenomic mRNA transcription from a defective interfering RNA.
... positive-strand RNA synthesis must start from the 5 9 -end of the genome (or 3 9 -end of the negative- strand template); thus, it should be regulated by the 5 9 - and not the 3 9 -end sequence of genomic ...of ... See full document
5
In Vitro- and In Vivo-Generated Defective RNAs of Satellite Panicum Mosaic Virus Define cis-Acting RNA Elements Required for Replication and Movement
... virus-specific replication of ...may function as a cis- acting factor for RNA stability or enhance replicase binding ...SPMV RNA syn- thesis, analogous to an interaction between ... See full document
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Characterization of a murine coronavirus defective interfering RNA internal cis-acting replication signal.
... positive-strand RNA synthesis but not for negative-strand RNA ...genomic RNA is sufficient for MHV negative-strand RNA synthesis ...positive-strand RNA synthesis? It is possible that ... See full document
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Host Protein Interactions with the 3′ End of Bovine Coronavirus RNA and the Requirement of the Poly(A) Tail for Coronavirus Defective Genome Replication
... in coronavirus defective genome ...important cis-acting signal for RNA ...BCV defective RNA replicon that lacked a poly(A) tail was not competent for replication, ... See full document
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Dependence of Coronavirus RNA Replication on an NH2-Terminal Partial Nonstructural Protein 1 in cis
... which RNA structures engage different components of the poly- merase ...higher-order RNA structure include much of the very 5= end of the genome which harbors the leader (nt 1 through 65) (7), the ... See full document
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cis Requirement for N-specific protein sequence in bovine coronavirus defective interfering RNA replication.
... DI RNA pDrep1 (4) had indicated that there was a strict ORF requirement for replication of the DI ...DI RNA and MHV DI RNA became ...DI RNA replication, we performed a series of ... See full document
7
An RNA Stem-Loop within the Bovine Coronavirus nsp1 Coding Region Is a cis-Acting Element in Defective Interfering RNA Replication
... order RNA structure identified as SLVI within the nsp1 coding region of the coronaviral genome that appears to act directly in cis for DI RNA ...occurring coronavirus DI RNAs (3, 4, 8) and in ... See full document
9
Stem-Loop IV in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... 2 coronavirus, human coronavi- rus HKU1 (39), also has a stem-loop IV homolog that varies only slightly from the other eight ...in coronavirus groups 1 and 3 and in the SARS coronavirus (see ...DI ... See full document
13
High-frequency leader sequence switching during coronavirus defective interfering RNA replication.
... Since the naturally occurring DI RNA has a deletion of nine nucleotides at the junction between the leader and the remaining genomic sequence and has three nucleotide substitutions withi[r] ... See full document
8
Location of cis-acting signals important for RNA encapsidation in the leader sequence of human immunodeficiency virus type 2.
... cytoplasmic RNA of an infected cell but constitutes approximately half of the RNA found within the virion (19, ...recognizes cis-acting RNA signals that are unique to the viral genomic ... See full document
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Defective Interfering Passages of Sindbis Virus: Nature of the Intracellular Defective Viral RNA
... For 1189 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest BHK cells infected with defective-interfering passages of Sindbis virus accumulate a species of RNA 20S that is[r] ... See full document
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A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.
... Construction of infectious cDNA clones. Complementary DNAs to the polio- virus genomes were prepared by using Amersham’s cDNA Synthesis System Plus. The cDNAs having EcoRI adaptors at both ends were inserted into the ... See full document
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cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication.
... Since all the amino acid replacement mutants that introduced charges in the hydrophobic domain of 3AB have a defect in vRNA synthesis, it was of interest to determine whether this defect[r] ... See full document
13
Isolation and Characterization of an Arterivirus Defective Interfering RNA Genome
... ence of sufficient helper virus. A representative example of the analysis of EDIC2-driven CAT expression during P0 is shown in Fig. 6B. In helper virus-infected cells but not in mock- infected cells, significant CAT ... See full document
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A hepatitis C virus cis acting replication element forms a long range RNA RNA interaction with upstream RNA sequences in NS5B
... Substitution of the CRE with the analogous region of different genotypes of HCV produced replicons with phenotypes consistent with the hypothesis that both local and long-range interactions are critical for a fundamental ... See full document
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