[PDF] Top 20 Analysis of the Viral Elements Required in the Nuclear Import of HIV-1 DNA
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Analysis of the Viral Elements Required in the Nuclear Import of HIV-1 DNA
... that nuclear entry defects were observed in viruses devoid of the same element in cycling and arrested cells may suggest that an active nuclear import through the NPC is required even in ... See full document
11
Inhibition of HIV 1 integrase nuclear import and replication by a peptide bearing integrase putative nuclear localization signal
... IN nuclear import is expected to result in the inhibition of virus replication, especially in cell-cycle arrested ...in HIV-1 infection--as is reflected by the inhibition of reporter gene ... See full document
16
Intermolecular masking of the HIV 1 Rev NLS by the cellular protein HIC: Novel insights into the regulation of Rev nuclear import
... Transient DNA transfections were performed using FuGENE6 (Roche Diagnostics, Mannheim, Germany) according to the manufacturer ’ s ...of DNA was equilibrated by addition of parent ...RT-PCR analysis ... See full document
13
Residual HIV 1 DNA Flap independent nuclear import of cPPT/CTS double mutant viruses does not support spreading infection
... of viral pre-integration complexes might enter the nucleus in the absence of the central DNA Flap, we could not exclude that a small proportion of DNA cir- cles form independently of nuclear ... See full document
13
Insights into the mechanisms of HIV-1 cis elements and trans factors required for RNA encapsidation and transduction
... nascent viral particle initiates infection through interaction of the envelope glycoproteins with host cell receptors on the plasma membrane (retroviral replication seen in ...The viral envelope fuses with ... See full document
192
Evidence for a Functional Link between Uncoating of the Human Immunodeficiency Virus Type 1 Core and Nuclear Import of the Viral Preintegration Complex
... Biochemical analysis of wild-type and Q63/67A ...and viral cDNA was quantified in the gra- dient fractions by real-time ...viruses, viral DNA (late reverse transcripts) was detected primarily ... See full document
9
Quantitative analysis of the time course of viral DNA forms during the HIV 1 life cycle
... that 1-LTRc formation involves the reverse tran- scription step in the cytoplasm compartment ...cleus, nuclear import has occurred at this time point ...the nuclear fraction (>99.5%) ... See full document
18
Nuclear Import Defect of Human Immunodeficiency Virus Type 1 DNA Flap Mutants Is Not Dependent on the Viral Strain or Target Cell Type
... type 1 (HIV-1) strain LAI, that the HIV-1 central DNA Flap acts as a cis determinant of viral genome nuclear ...the DNA Flap on nuclear import ... See full document
8
The Cargo-Binding Domain of Transportin 3 Is Required for Lentivirus Nuclear Import
... the nuclear envelope during mitosis to access the target cell chromosomal DNA (26, 35), lentiviruses transit through the nuclear pore, allowing them to infect terminally differentiated and ... See full document
12
The dimerization domain of HIV 1 viral infectivity factor Vif is required to block virion incorporation of APOBEC3G
... of viral infectivity by the peptide was signifi- cantly greater (p ≤ ...reduced viral infectivity of +Vif virus produced in cells lacking ...supported viral infectivity through an hA3G-inde- pendent ... See full document
11
Contribution of the C terminal tri lysine regions of human immunodeficiency virus type 1 integrase for efficient reverse transcription and viral DNA nuclear import
... protein nuclear local- ization and their inability to bind to karyopherin α in vitro ...during HIV-1 replication, other studies did not reveal the importance of these IN mutants (K186Q and Q214/216L) ... See full document
15
Reassessment of the Roles of Integrase and the Central DNA Flap in Human Immunodeficiency Virus Type 1 Nuclear Import
... the viral life cycle in immortalized cell lines but does delay replication modestly in primary cell cul- tures ...PIC nuclear import in these cells, we propose that disruption of the cPPT may render ... See full document
10
Nuclear Export Signal Masking Regulates HIV-1 Rev Trafficking and Viral RNA Nuclear Export
... to HIV-1 Rev or doubling the number of RRE sequences per viral RNA transcript was sufficient to overcome a profound block to HIV-1 in murine cells, attributable to a species-specific ... See full document
19
The Positively Charged Termini of L2 Minor Capsid Protein Required for Bovine Papillomavirus Infection Function Separately in Nuclear Import and DNA Binding
... the viral DNA or nuclear import of L2? To address this question, we tested an L2 mutant encoding a scrambled version of the cNLS, which supports the production of infectious virions ...the ... See full document
8
Mutations affecting interaction of integrase with TNPO3 do not prevent HIV 1 cDNA nuclear import
... of HIV-1 replication using real-time quantitative ...using HIV-1 gp120 envelope suggesting that IN muta- tions could affect pre-integration steps specific to the VSVg-mediated endocytic ... See full document
14
Autographa californica Multiple Nucleopolyhedrovirus DNA Polymerase C Terminus Is Required for Nuclear Localization and Viral DNA Replication
... in viral DNA replication and shows high similarity to DNApols of other DNA viruses, such as adenovirus 7 and herpes simplex virus 1, and even Archaea ...for viral DNA rep- ... See full document
16
Evaluation of the Functional Involvement of Human Immunodeficiency Virus Type 1 Integrase in Nuclear Import of Viral cDNA during Acute Infection
... of HIV-1 IN identified three functional domains (reviewed in references 35 and 38): an N-terminal zinc binding domain, a central catalytic core domain, and a C-terminal nonspecific DNA binding ...of ... See full document
11
The HIV 1 Rev/RRE system is required for HIV 1 5' UTR cis elements to augment encapsidation of heterologous RNA into HIV 1 viral particles
... The HIV-1 Rev protein mediates nuclear export of unspliced full-length, as well as partially spliced, viral RNAs by bridging an interaction between the Rev response element (RRE) in the gRNA ... See full document
17
SUN1 Regulates HIV-1 Nuclear Import in a Manner Dependent on the Interaction between the Viral Capsid and Cellular Cyclophilin A
... of HIV-1 nuclear entry, we used siRNAs to downregulate SUN1 expression and analyzed the levels of the late RT product, 2-LTR DNA, integrated viral DNA, viral mRNAs, and ... See full document
15
TNPO3 Is Required for HIV-1 Replication after Nuclear Import but prior to Integration and Binds the HIV-1 Core
... panel). HIV-1, HIV-1 with an N74D mutation (HIV-1 N74D), and HIV-1 Q63A/ Q67A were normalized by quantifying the particle-associated re- verse transcriptase ... See full document
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