[PDF] Top 20 Cell-Free Replication of the Hepatitis C Virus Subgenomic Replicon

... the replicon RNA in FCA4 lysates is present in a nuclease-resistant replication com- ...liovirus replication complexes from the HeLa CTR ...incomplete replication buffer and subjected to the ... See full document

... was replication competent in the human hepatoma cell line Huh-7, and adaptive mutations in the HCV-encoded proteins were required for efficient HCV replication (2, 9, 13, ...ditional cell ... See full document

... independent replicon cell ...selectable subgenomic replicons into Huh-7 cells and selection with G418, we found that only a small number of colonies formed that supported persistent ... See full document

... founder cell line 5–15 ...for cell culture adaptation, they were introduced individually into the parental replicon construct and serial dilutions of in vitro transcripts were trans- fected into ... See full document

... Kunjin virus replication (15, 22), we first determined the effect of VS on HCV replicon RNA ...HCV replicon cells were plated at 12,000 cells per well in a 96-well plate in Dulbecco’s modified ... See full document

... to replication and that the molecules were not affecting translation by 39UTR or 59UTR binding, we performed translation assays with bicistronic ...(hepatitis C virus, human rhinovirus), with ... See full document

... RNA replication machinery in human ...HCV replication due to the lack of an efficient cell culture ...RNA replicon provides a suitable model to test our hypothesis (2, 12, ...HCV ... See full document

... osteosarcoma-derived cell lines induc- ibly expressing the HCV polyprotein ...these cell lines, the membranous web was often found closely associated with the rough endoplasmic reticulum ...in ... See full document

... SGR plasmids were linearized with Xba I (New England Biolabs) and RNA was transcribed using a T7 transcription kit (Promega) according to the manufacturer ’ s instructions. Two micrograms of RNA transcripts were ... See full document

... atoma cell lines, including Huh-7 ...on virus replication in hepa- ...in replicon-harboring ...NV replicon-harboring ...HCV replication, the effective concentrations of various ... See full document

... intracellular subgenomic HCV RNA levels in a biphasic-like ...parallel replicon cell cultures were treated with the same four IFN- ␣ concentrations tested above, ...the replicon cells treated ... See full document

... RNA replication (4, 9). For instance, HCV RNA replication has been reported to occur in association with detergent-resistant membranes, believed to be enriched in unesteri- fied/free cholesterol and ... See full document

... the hepatitis C virus (HCV) genome is highly conserved and contains specific cis-acting RNA motifs that are essential in directing the viral replication machinery to initiate at the correct 3 ... See full document

... host cell antiviral response through a variety of processes that lead to the activation of transcription factors whose actions promote the expression of alpha-beta interferons (IFN-␣/␤) and IFN-stimulated genes ... See full document

... RNA replication independently from IRES-dependent translation and RNA ...HCV replication upon DDX60L expression sug- gested that DDX60L acts as a direct effector protein inhibiting HCV replication, ... See full document

... on hepatitis C virus (HCV) replication have been greatly advanced by the development of cell culture models for HCV known as replicon ...prototype replicon consists of a ... See full document

... atitis C virus (HCV) infection, the detailed anti-HCV effect of ribavirin in clinical concentrations remains ...reporter replicon system with an HCV genotype 2a subgenomic replicon ... See full document

... HCV replication complex is very similar to a model suggested by Miyanari et ...HCV replication complex is composed of multiple copies of the viral nonstructural ...in replicon cells: the ... See full document

... RNA replication of ...the cell surface were quantified by determining the amount of viral genomic RNA by ...JFH1-derived subgenomic replicon were transfected with MxB-Flag plasmid DNA, followed ... See full document

... used cell-free supernatants derived from several cell lines with an sfl genome to infect parental Huh-7 ...a cell line carrying a subgenomic HCV replicon were applied in ...and ... See full document