[PDF] Top 20 Characterization of Clonal Complexity in Tuberculosis by Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Typing

... subtle clonal variants should not be consid- ered to be caused by random variations due to instability of MIRUs, as it has been stated that these markers have higher stabilities than that of RFLP ... See full document

... This study aimed at determining the prevalence of infection with multiple MTB strains in a high TB inci- dence setting in Kampala (Kawempe division), Uganda, using “ Mycobacterial Interspersed ... See full document

... Mycobacterium tuberculosis complex ge- nome, described as mycobacterial interspersed repetitive units, may show polymorphism of the number of tandem ...M. tuberculosis ... See full document

... Mycobacterium tuberculosis. However, due to the complexity of the IS6110 RFLP genotyping technique, and the interpretation of RFLP data, mycobacterial interspersed ... See full document

... (iii) MIRU-VNTR. The primers reported by Thibault et al. (25) were used for MIRU-VNTR typing analysis (see Table S1 in the supplemental material). The PCR mixture (25 ␮ l) was composed of 1 ␮ l of DNA solution, ... See full document

... (MDR) tuberculosis (TB) is a serious health threat that requires molecular procedures yielding results quickly to improve control of the diffusion of drug-resis- tant strains ...Mycobacterium tuberculosis ... See full document

... MIRU-VNTR typing was performed at Genoscreen on a total of 638 isolates and in parallel by each reference center on its own isolate set (except for the outbreak panel, for which typing was performed only at ... See full document

... RFLP typing, PCR-based MIRU-VNTR typing or spoligotyping can be applied with crude DNA extracts from heat-killed mycobacterial cultures as soon as they become positive, avoiding culture delays of ... See full document

... MIRU-VNTR typing combined with spoligotyping as a first-line approach provides adequate discrimination in most cases for large-scale genotyping of ...M. tuberculosis in the United States ...VNTR ... See full document

... the number of copies of tandem repeat sequences is an invaluable tool for the genotyping of higher ...M. tuberculosis (28). A limited number of these structures (five in total) were ... See full document

... MIRU typing did not provide suf- ficient resolution for the subtyping of Beijing genotype ...M. tuberculosis strains compared with IS6110 RFLP, the advan- tages of VNTR typing over RFLP are obvious; ... See full document

... the typing of a panel of ...Mycobacterium tuberculosis (35), a consensus-based MIRU- VNTR typing method could thus become the new standard ... See full document

... Molecular typing allows a rapid and precise species differentia- tion and is essential in investigating the spread of specific genotypes and any relationship with drug ...Mycobacteria interspersed ... See full document

... recurrent tuberculosis can be chronically infected with a given strain and relapse due to reactivation of that strain or, in contrast, can be reinfected by a different strain after cure ...of tuberculosis ... See full document

... rep-PCR. rep-PCR was very reproducible, with both transpar- ent and opaque colony variants from a single genotype done on three separate occasions with different growth times and DNA preparation showing indistinguishable ... See full document

... The next step was to evaluate the distribution of cases iden- tified as clustered or orphan by each of the techniques evalu- ated. MIRU-VNTR genotyping has been reported to identify as many clustered cases as RFLP ... See full document

... Mycobacterium tuberculosis based on single nucleotide polymorphism (SNP) analysis: insights into tuberculosis evolution, phylogenetic accu- racy of other DNA fingerprinting systems, and recommendations for ... See full document

... M. tuberculosis in Singapore. Using MIRU-VNTR typing, we analyzed 303 ...M. tuberculosis isolates from 291 ...VNTR typing was high (HGDI ⫽ 0.975) for this M. tuberculosis sample ... See full document

... Standardized mycobacterial interspersed repetitive-unit–variable-number tandem repeat (MIRU-VNTR) typing based on 15 and 24 loci recently has been proposed ... See full document

... Sensitivity and specificity are measures of how well a new test performs in securing a diagnosis. In the case of tubercu- losis molecular epidemiology, an insensitive technique will over- look transmission events, while ... See full document