[PDF] Top 20 Characterization of the DNA binding properties of polyomavirus capsid protein.
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Characterization of the DNA binding properties of polyomavirus capsid protein.
... The major viral structural protein VP1 and host-contributed histone proteins of polyomavirus virions were shown to exhibit DNA binding activity, but the minor capsid proteins VP2 and VP3[r] ... See full document
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SYNTHESIS, SPECTRAL CHARACTERIZATION AND DNA BINDING PROPERTIES OF LANTHANIDE (III) COMPLEXES WITH 2 FORMYLPYRIDINE NICOTINOYLHYDRAZONE
... spectral characterization and DNA binding properties of lanthanide (III) complexes of 2-formylpyridine nicotinoylhydrazone for the first ... See full document
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SPECTRAL CHARACTERIZATION AND DNA BINDING PROPERTIES OF LANTHANIDE (III) COMPLEXES WITH 2 ACETYLPYRIDINE NICOTINOYLHYDRAZONE (APNH)
... on DNA binding activities of lanthanide(III) metal complex, herein we report synthesis, spectral characterization and DNA binding properties of lanthanide(III) complexes of 2- ... See full document
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Characterization of the DNA-binding properties of the polyomavirus capsid protein VP1.
... A nitrocellulose transfer assay using purified recombinant VP1 protein was developed for detecting the DNA binding of VP1 transferred to nitrocellulose after SDS-PAGE Southwestern assay.[r] ... See full document
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Functional and Genetic Characterization of the Oligomerization and DNA Binding Properties of the Drosophila Doublesex Proteins
... Ph.D. Dissertation, University of California, Davis. BURTIS, 1993 The Drosophila doublesex proteins share a novel zinc finger related DNA binding domain. GOLEMIS, 1995 Correla[r] ... See full document
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Characterization of the DNA-Binding Properties of the Origin-Binding Domain of Simian Virus 40 Large T Antigen by Fluorescence Anisotropy
... detect binding of full-length T antigen to fluorescent probes under the same salt ...increase binding of T antigen to the probe, we performed binding isotherms at lower salt concen- trations, either ... See full document
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Characterization of the DNA-binding properties of herpes simplex virus regulatory protein ICP4.
... DNAbinding immunoassays with fraction VIII and portions of the HSV genome revealed stable complexes that contained ICP4 and specific sequences in the promoters for viral genes encoding I[r] ... See full document
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Characterization of the Single-Stranded DNA Binding Protein Encoded by the Vaccinia Virus I3 Gene
... with DNA in ...inherent DNA binding ...including DNA replication, may be regulated through modulation of hRPA ...its DNA binding ...mediates protein-protein ... See full document
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Nuclear assembly of polyomavirus capsids in insect cells expressing the major capsid protein VP1.
... As a test of the intrinsic in vivo assembly properties of a DNA virus coat protein, we expressed the polyomavirus capsid protein VP1 in insect cells using a baculovirus vector 9, 30 and [r] ... See full document
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Characterization of two novel YY1 binding sites in the polyomavirus late promoter.
... free DNA by electrophoresis on a 5% nondenaturing polyacrylamide gel and visualized by ...autoradiography. DNA- protein complexes were competed for with a 50-, 100-, or 500-fold molar excess of the ... See full document
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Functional Characterization of Three Leishmania Poly(A) Binding Protein Homologues with Distinct Binding Properties to RNA and Protein Partners
... PCR and cloning. The coding sequences for the three L. major PABP homo- logues were amplified from total DNA extracted from the Friedlin strain. The LmPABP1 and LmPABP2 sequences were both amplified through two ... See full document
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Progressive Multifocal Leukoencephalopathy Associated Mutations in the JC Polyomavirus Capsid Disrupt Lactoseries Tetrasaccharide c Binding
... JC polyomaviruses with PML-associated mutations are not in- fectious. Mutations in JCPyV VP1 arise in individuals with PML at a very high frequency. In fact, 80 to 90% of viral isolates from individuals with PML exhibit ... See full document
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Isolation and Characterization of the DNA and Protein Binding Activities of Adenovirus Core Protein V
... basic protein with a calculated pI of ...1A). Protein V contains multiple nuclear and nucleolar localization signals and is im- ported into the nucleus very shortly after viral infection, suggest- ing that ... See full document
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Polyomavirus major capsid protein VP1 is capable of packaging cellular DNA when expressed in the baculovirus system.
... major capsid protein gene only, and the minor capsid proteins or the T antigens were not ...the capsid- like particles we found the presence of host-derived histones in addition to cellular ... See full document
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Expression of the human papillomavirus type 11 L1 capsid protein in Escherichia coli: characterization of protein domains involved in DNA binding and capsid assembly.
... major capsid protein of human papillomavirus type 11 (HPV-11) was expressed in Escherichia coli, and the soluble recombinant protein was purified to near ...L1 protein bound DNA as ... See full document
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... pLTR880 (a gift from T. Roberts, Dana-Farber Cancer Institute) expresses LT from a cDNA. The vector consists of pBR322 sequences, the Harvey sarcoma- virus long terminal repeat for transcription initiation, and an LT ... See full document
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Synthesis, Characterization, DNA binding and spectral properties of complex [Ru(py)4(dppz)]2+
... 2.3d DNA Photocleavage: Irradiation of sample containing pBR 322 DNA and the complex were carried out for ...plasmid DNA was monitored by agarose gel ...plasmid DNA was subjected to gel ... See full document
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Molecular Genetic and Biochemical Characterization of the Vaccinia Virus I3 Protein, the Replicative Single-Stranded DNA Binding Protein
... double-stranded DNA tem- plate, as well as solving the somewhat unique problems presented by replication within the cytoplasm, such as evading cytoplasmic DNA sensors ...I3 protein (6), providing a ... See full document
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Identification and Characterization of Antibody-Binding Epitopes on the Norovirus GII.3 Capsid
... (MAb) binding to expressed capsid pro- teins or short peptides, phage display, targeted mutagenesis, and crystallography (22, 23, ...viral binding to HBGAs) have been mapped to the P2 domain (34, ... See full document
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Identification and Molecular Characterization of the RNA Polymerase-Binding Motif of Infectious Bursal Disease Virus Inner Capsid Protein VP3
... Since the VP3 mutant containing the smallest deletion was completely unable to bind VP1, the possibility that the PKR and/or arginine-rich regions might also play a role in the in- teraction with VP1 could not be ruled ... See full document
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