[PDF] Top 20 Comparative Analysis of Adeno-Associated Virus Capsid Stability and Dynamics

... of adeno-associated virus (AAV), AAV1, AAV2, AAV5, and AAV8, were compared with respect to the physical properties of their capsids that influence thermody- namic ...Thermal stability ... See full document

... In the case of the AAV4 and AAV5 HI loops, significant phenotypic variations were seen, possibly due to lower se- quence homology with AAV2. For instance, the AAV4 HI loop is composed of a higher number of hydrophobic ... See full document

... MS analysis to identify the ap- proximate positions of the cleavage sites (see ...the capsid protein, as expected, but also generated peptides located in the middle of the capsid and at the N ... See full document

... VP stability and in VP oligomerization during assembly 10,52 ...a capsid is entirely a property of ...in capsid assembly, we expressed it in ...in capsid assembly, next to its before described ... See full document

... of adeno-associated virus (AAV) are packaged into preformed ...the capsid, thereby targeting the genome to the portal of ...the capsid protein ...of capsid protein pentamers led ... See full document

... Antibody binding to AAV2. The C37-B MAb is specific for AAV2 and does not cross-react with other serotypes tested (50). Density for the Fab was observed at the top of each of three pro- trusions surrounding the 3-fold ... See full document

... immunoblot analysis using the monoclonal mouse antibody A20, which recognizes a conformational epitope on the intact capsid, and the monoclonal mouse antibody A1, which rec- ognizes a linear epitope in the ... See full document

... AAV2 capsid assembly (17), this implies that AAP exerts its main function by mechanisms different from mere stimulation of VP protein expression or intracellular targeting (see also point iii above and the next ... See full document

... DNA) virus-like particles (VLPs) as well as green fluorescent protein (GFP) gene-packaged (DNA full; recom- binant AAV8 [rAAV8]-GFP) capsids at pH ...the capsid structures of AAV8 VLPs and rAAV8-GFP ... See full document

... Adeno-associated virus type 2 (AAV2) capsid assembly requires the expression of a virally encoded assembly- activating protein ...the capsid protein VP3, capsids are formed that are ... See full document

... 1428 and 4495 nucleotides of AAV genome (numbering is as for the sequence under GenBank accession no. AF043303.1) was cloned into YEplac181. The resulting plasmid named YEplacp40Cap has the yeast 2-micron ori- gin of ... See full document

... Our data suggest that, in the retina, the binding of the AAV capsids to the cell is not the sole determinant of transduction efficiency. We found that AAV2 transgene expression could be enhanced by reduction in genome ... See full document

... other capsid VP regions and the overall capsid, including the packaged ...An analysis of the calculated pI values of different capsid regions for AAV serotypes 1 to 12 showed that VP1u has an ... See full document

... TT comparative biodistribution studies, we assessed the ef- ficacy of AAV-TT in correcting neurological deficits in the mouse model of MPSIIIC by comparing it to preferred neurological ... See full document

... Analysis of molecular forms of genotyped AAV vector DNA in stably transduced liver. Our finding of identical hFIX pro- tein expression profiles within a dose cohort, regardless of the vector genotype, suggested ... See full document

... These mutants were located predominantly, but not exclusively, in what are likely to be ␤ -barrel structures in the capsid protein VP3. Two of these mutants were insertions at the N and C termini of VP3, ... See full document

... 2006 Adeno-associated virus type 2 (AAV-2) capsid proteins have eight sequence motifs that are potential sites for O- or N-linked ...progeny virus. Peptide mapping and structural ... See full document

... Adeno-associated virus (AAV) is gaining momentum as a gene therapy vector for human ...this virus as a ...the virus, including nuclear targeting of the incoming virion during initial ... See full document

... thorough analysis, the prefer- ence for the 3⬘ crossover point was most likely due to the fact that this region contained the largest stretch of homology be- ...rescued virus from independent marker rescue ... See full document

... the capsid uncoating processes, essential for gene expression inside host ...preserve capsid geometry, only those residues that lie outside the interaction interfaces in the phosphodegron can be selected ... See full document