[PDF] Top 20 Composition and assembly of the yeast vacuolar H(+) ATPase complex

... the yeast Saccharomyces cerevisiae is one of the most extensively characterized members of the V- type ATPase family ...In yeast, the V-ATPase functions to acidify the vacuole by driving the ... See full document

... In yeast, subunit a is encoded by two homologous genes (VPH1 and STV1) which encode proteins that are targeted to different intracellular destinations (Manolson et ...in assembly of the V-ATPase ... See full document

... The F- and V-ATPases are protein complexes related both in their subunit structure and in their evolutionary origin (Nelson, 1992a,b,c). Moreover, it is assumed that their mechanism of action is similar. Both enzymes ... See full document

... 10 h) (Fig. 5B, top panel) and TLS2 mutant (by 6 h) ...from yeast spheroplasts were immunoprecipitated under native conditions with 12CA5 antibody ...Skp1-Skp2 complex is depicted with the ... See full document

... rotary ATPase structures is implicated is in accommodating the large-scale conformational changes that occur sequentially in the soluble domain during the catalytic ...V- ATPase subunit A (containing ... See full document

... V-ATPase complex, revealing insights into its structure and ...full complex. Due to the speed of the V-ATPase catalytic cycle and the currently limiting freezing speeds of vitrification ... See full document

... (data not shown), consistent with results from immuno- fluorescence microscopy with anti-B-subunit antibody (Kane et al. 1992). As reported previously for a number of the strains (Raymond et al. 1992; Seeley et al. ... See full document

... Upon nutrient limitation, budding yeast cells can switch to filamentous growth (13, 49). This is characterized by cell elonga- tion, a switch to unipolar budding, increased cell-cell adhesion, increased ... See full document

... the assembly state of the V-ATPase in the presence of 2- deoxyglucose, which can be imported and phosphorylated but cannot be further ...a yeast phosphoglucoisomerase (pgi1) mutant, which cannot ... See full document

... The assembly of the yeast vacuolar H + -ATPase requires the attachment of several cytoplasmically oriented, peripheral subunits the Vi sector to a complex of integral membrane subunits t[r] ... See full document

... V-ATPase subunits (Figure 3, lanes 5 and 6). These results suggest that the VPH6 protein plays a role in vacuolar H'-ATPase assembly. Yeast strains with defects in va[r] ... See full document

... the vacuolar or V-ATPase was isolated from the column elution step, suggesting that it was indeed captured as a bio- tin-labeled cross-linked complex with ...protein complex known to be ... See full document

... DeWitt et al., 1998; Sen Gupta et al., 1998). The same mutants are exceedingly sensitive to low concentrations of trypsin, consistent with the idea that they are poorly folded and arrested by the endoplasmic reticulum ... See full document

... Receptor recycling assay was performed as previously described [25]. COS7 cells were plated onto poly-L- lysine coated coverslips and infected with lentivirus as described above. Forty-eight hours after infection, cells ... See full document

... the ATPase do- main of the proton-pumping F1-ATPase, which in turn is highly similar to that of Vacuolar-type H + -ATPase (V-ATPase) ... See full document

... cross-reactive protein in discrete vesicles and unpolarized OC cultured on glass which redistributed toward the periphery of cells exposed to bone matrix. Na-dependent Pi transport in the OC was stimulated in the ... See full document

... Na/K- ATPase activity in the medullary collecting tubule; this stimulatory effect of hypokalemia supports the hypothesis that the enzyme is present on the apical ... See full document

... the rat epididymis express high levels of V-ATPase at their apical pole for proton secretion that, in the vas deferens, is required for sperm maturation and storage (Brown and Breton, 1996). It has also been shown ... See full document

... The mechanism for sensing and counteracting blood alkalosis in VHA-rich gill cells is likely to be applicable to other epithelial base- secreting cells. For example, β -intercalated cells ( β -ICs; see Glossary) in the ... See full document

... predicted size of 95·kDa was demonstrated by SDS-PAGE analysis of whole-cell extracts. The fusion protein construct was verified by dideoxynucleotide sequencing. The fusion protein was purified on amylose columns, using ... See full document